Strain identifier

BacDive ID: 13062

Type strain: Yes

Species: Pseudomonas graminis

Strain Designation: P 294/08, P294/08

Strain history: CIP <- 1999, U. Behrendt, ZALF, Müncheberg, Germany: strain P294/08 <- DSMZ <- Paulinenaue

NCBI tax ID(s): 158627 (species)

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General

@ref: 4311

BacDive-ID: 13062

DSM-Number: 11363

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas graminis P 294/08 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from surface plant material from a mixed meadow.

NCBI tax id

  • NCBI tax id: 158627
  • Matching level: species

strain history

@refhistory
4311<- U. Behrendt, ZALF Müncheberg, P 294/08
119726CIP <- 1999, U. Behrendt, ZALF, Müncheberg, Germany: strain P294/08 <- DSMZ <- Paulinenaue

doi: 10.13145/bacdive13062.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas graminis
  • full scientific name: Pseudomonas graminis Behrendt et al. 1999

@ref: 4311

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas graminis

full scientific name: Pseudomonas graminis Behrendt et al. 1999

strain designation: P 294/08, P294/08

type strain: yes

Morphology

cell morphology

  • @ref: 119726
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 119726
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4311REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
39760MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119726CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119726CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4311positivegrowth28mesophilic
39760positivegrowth25mesophilic
59215positivegrowth30mesophilic
119726positivegrowth5-37
119726nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59215aerobe
119726obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119726NaClpositivegrowth0 %
119726NaClnogrowth2 %
119726NaClnogrowth4 %
119726NaClnogrowth6 %
119726NaClnogrowth8 %
119726NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11972616947citrate+carbon source
1197264853esculin+hydrolysis
11972617632nitrate-reduction
11972616301nitrite-reduction
11972617632nitrate-respiration

antibiotic resistance

  • @ref: 119726
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119726
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
119726oxidase-
119726beta-galactosidase-3.2.1.23
119726alcohol dehydrogenase-1.1.1.1
119726gelatinase+/-
119726amylase-
119726DNase-
119726caseinase-3.4.21.50
119726catalase+1.11.1.6
119726tween esterase+
119726lecithinase-
119726lipase-
119726lysine decarboxylase-4.1.1.18
119726ornithine decarboxylase-4.1.1.17
119726protease-
119726tryptophan deaminase-
119726urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119726--++-++---++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119726+++-+-------------++++--+-+++--+++--++-+---++-+-++++-++++---+----------+++-++++++++++-++++++++-++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4311surface plant material from a mixed meadowBrandenburg, Havelländisches LuchGermanyDEUEurope
59215Surface plant material from a mixed meadowGermanyDEUEurope
119726GrassesGermanyDEUEurope1993

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Host#Plants

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43111Risk group (German classification)
1197261Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas graminis strain DSM 11363 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ023307598ena158627
20218Pseudomonas graminis 16S rRNA geneY111501529ena158627

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas graminis DSM 11363GCA_900111735scaffoldncbi158627
66792Pseudomonas graminis strain DSM 11363158627.14wgspatric158627

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes92.34no
flagellatedyes78.738no
gram-positiveno97.987no
anaerobicno98.2yes
aerobicyes92.426yes
halophileno92.017no
spore-formingno93.942no
glucose-fermentno90.521no
thermophileno99.884no
glucose-utilyes95.216no

External links

@ref: 4311

culture collection no.: DSM 11363, CCUG 51504, CIP 105897, CCM 8843

straininfo link

  • @ref: 82272
  • straininfo: 49084

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028275A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov.Behrendt U, Ulrich A, Schumann P, Erler W, Burghardt J, Seyfarth WInt J Syst Bacteriol10.1099/00207713-49-1-2971999Base Sequence, DNA, Bacterial/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Poaceae/*microbiology, Pseudomonas/*classification/cytology/physiology, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny12656147Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJInt J Syst Evol Microbiol10.1099/ijs.0.02326-02003Agriculture, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Korea, Microscopy, Electron, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species SpecificityGenetics
Phylogeny14657147Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro.Peix A, Rivas R, Mateos PF, Martinez-Molina E, Rodriguez-Barrueco C, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.02703-02003Base Composition, Burkholderia/classification, Catalase/metabolism, Molecular Sequence Data, Phosphates/*metabolism, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification/metabolism, RNA, Bacterial/chemistry/genetics, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny15143034Pseudomonas lutea sp. nov., a novel phosphate-solubilizing bacterium isolated from the rhizosphere of grasses.Peix A, Rivas R, Santa-Regina I, Mateos PF, Martinez-Molina E, Rodriguez-Barrueco C, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.02966-02004Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phosphates/metabolism, Phylogeny, Poaceae/microbiology, Pseudomonas/*classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Solubility, SpainGenetics
Phylogeny29867824Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.Saati-Santamaria Z, Lopez-Mondejar R, Jimenez-Gomez A, Diez-Mendez A, Vetrovsky T, Igual JM, Velazquez E, Kolarik M, Rivas R, Garcia-Fraile PFront Microbiol10.3389/fmicb.2018.009132018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4311Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11363)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11363
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39760Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17967
59215Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51504)https://www.ccug.se/strain?id=51504
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
82272Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49084.1StrainInfo: A central database for resolving microbial strain identifiers
119726Curators of the CIPCollection of Institut Pasteur (CIP 105897)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105897