General

@ref: 21732

BacDive-ID: 130589

DSM-Number: 46664

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Prauserella isguenensis H225 is a mesophilic bacterium that was isolated from desert soil.

NCBI tax id

• NCBI tax id: 1470180
• Matching level: species

strain history

• @ref: 21732
• history: <- N. Sabaou, LBSM, Ecole Normale Supérieure, Kouba, Alger, Algeria; H225

doi: 10.13145/bacdive130589.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/actinomycetota
• domain: Bacteria
• phylum: Actinomycetota
• class: Actinomycetes
• order: Pseudonocardiales
• family: Pseudonocardiaceae
• genus: Prauserella
• species: Prauserella isguenensis
• full scientific name: Prauserella isguenensis Saker et al. 2015

@ref: 21732

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella isguenensis

full scientific name: Prauserella isguenensis Saker et al. 2015

strain designation: H225

type strain: yes

Morphology

cell morphology

multimedia

• @ref: 21732
• multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46664.jpg
• caption: Medium 1504 28°C
• intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

• @ref: 21732
• name: CM-MEDIUM (DSMZ Medium 1504)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1504
• composition: Name: CM MEDIUM (DSMZ Medium 1504) Composition: NaCl 150.0 g/l Agar 20.0 g/l MgSO4 x 7 H2O 10.0 g/l Yeast extract 10.0 g/l Peptone 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O Distilled water

culture temp

• @ref: 21732
• growth: positive
• type: growth
• temperature: 28
• range: mesophilic

Isolation, sampling and environmental information

isolation

• @ref: 21732
• sample type: desert soil
• geographic location: Sahara, Mzab region, region of Ahbas (32° 27' N 3° 41' E) at Béni-Isguen (Ghardaia)
• country: Algeria
• origin.country: DZA
• continent: Africa
• latitude: 32.45
• longitude: 3.6833

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_41937.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_3364;97_4129;98_30482;99_41937&stattab=map
• Last taxonomy: Prauserella
• 16S sequence: KJ504173
• Sequence Identity:
• Total samples: 322
• soil counts: 41
• aquatic counts: 15
• animal counts: 255
• plant counts: 11

Safety information

risk assessment

• @ref: 21732
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21732
• description: Prauserella isguenensis strain H225 16S ribosomal RNA gene, partial sequence
• accession: KJ504173
• length: 1441
• database: ena
• NCBI tax ID: 1470180

Genome sequences

GC content

• @ref: 21732
• GC-content: 70.4
• method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

External links

@ref: 21732

culture collection no.: DSM 46664, CECT 8577

straininfo link

• @ref: 89104
• straininfo: 405355

literature

• topic: Phylogeny
• Pubmed-ID: 25713039
• title: Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil.
• authors: Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Sabaou N, Klenk HP
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.000145
• year: 2015
• mesh: Actinomycetales/*classification/genetics/isolation & purification, Algeria, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
• topic2: Genetics

Reference