Strain identifier

BacDive ID: 130585

Type strain: Yes

Species: Actinokineospora spheciospongiae

Strain Designation: EG49

Strain history: CCM 8480 <-- U. R. Abdelmohsen; Univ. Würzburg, Germany; EG49.

NCBI tax ID(s): 909613 (species)

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General

@ref: 21459

BacDive-ID: 130585

DSM-Number: 45935

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Actinokineospora spheciospongiae EG49 is a mesophilic bacterium that was isolated from marine sponge Spheciospongia vagabunda.

NCBI tax id

  • NCBI tax id: 909613
  • Matching level: species

strain history

@refhistory
21459<- U. R. Abdelmosen, University Würzburg; EG49
67770CCM 8480 <-- U. R. Abdelmohsen; Univ. Würzburg, Germany; EG49.

doi: 10.13145/bacdive130585.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinokineospora
  • species: Actinokineospora spheciospongiae
  • full scientific name: Actinokineospora spheciospongiae Kämpfer et al. 2015

  • synonyms

    • @ref: 20215
    • synonym: Actinokineospora mzabensis

@ref: 21459

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Actinokineospora

species: Actinokineospora spheciospongiae

full scientific name: Actinokineospora spheciospongiae Kämpfer et al. 2015 emend. Nouioui et al. 2018

strain designation: EG49

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 21459
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21459positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H6), MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21459-++/-++/-++/-+/-+/-+/-+/-----+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
21459-----+/---------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
21459marine sponge Spheciospongia vagabundaSpheciospongia vagabundaRed Sea, Sinai, Ras Mohamed (27° 47.655' N 34° 12.904' E)EgyptEGYAfrica27.794334.2151
67770Marine sponge Spheciospongia vagabunda from the Red SeaSpheciospongia vagabundaRas Mohamed, SinaiEgyptEGYAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Invertebrates (Other)#Porifera (Sponges)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5405.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_394;97_444;98_4031;99_5405&stattab=map
  • Last taxonomy: Actinokineospora
  • 16S sequence: GU318361
  • Sequence Identity:
  • Total samples: 82
  • soil counts: 34
  • aquatic counts: 9
  • animal counts: 39

Safety information

risk assessment

  • @ref: 21459
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21459
  • description: Actinokineospora spheciospongiae strain EG49 from Egypt 16S ribosomal RNA gene, partial sequence
  • accession: GU318361
  • length: 1481
  • database: ena
  • NCBI tax ID: 909613

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinokineospora spheciospongiae EG492590828683draftimg909613
67770Actinokineospora spheciospongiae EG49GCA_000564855contigncbi909613

GC content

  • @ref: 67770
  • GC-content: 72.8
  • method: genome sequence analysis

External links

@ref: 21459

culture collection no.: DSM 45935, CCM 8480, LMG 27700, JCM 31756

literature

  • topic: Phylogeny
  • Pubmed-ID: 25519300
  • title: Actinokineospora spheciospongiae sp. nov., isolated from the marine sponge Spheciospongia vagabunda.
  • authors: Kampfer P, Glaeser SP, Busse HJ, Abdelmohsen UR, Ahmed S, Hentschel U
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000031
  • year: 2014
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Indian Ocean, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21459Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45935Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45935)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/