Strain identifier
BacDive ID: 130584
Type strain:
Species: Actinokineospora spheciospongiae
Strain history: <- N. Sabaou, Kouba,Alger,Algeria; PAL84
NCBI tax ID(s): 909613 (species)
General
@ref: 21458
BacDive-ID: 130584
DSM-Number: 45961
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Actinokineospora spheciospongiae DSM 45961 is a spore-forming, mesophilic bacterium that was isolated from Saharan soil from a palm grove.
NCBI tax id
- NCBI tax id: 909613
- Matching level: species
strain history
- @ref: 21458
- history: <- N. Sabaou, Kouba,Alger,Algeria; PAL84
doi: 10.13145/bacdive130584.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinokineospora
- species: Actinokineospora spheciospongiae
- full scientific name: Actinokineospora spheciospongiae Kämpfer et al. 2015
synonyms
- @ref: 20215
- synonym: Actinokineospora mzabensis
@ref: 21458
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Actinokineospora
species: Actinokineospora mzabensis
full scientific name: Actinokineospora mzabensis Aouiche et al. 2015
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 93.892 | |
69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 21458
- name: ISP2 MEDIUM (DSMZ Medium 987)
- growth: yes
- link: https://mediadive.dsmz.de/medium/987
- composition: Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
culture temp
- @ref: 21458
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | + | hydrolysis |
68369 | 16199 | urea | + | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | gelatinase | + | |
68369 | beta-glucosidase | + | 3.2.1.21 |
68369 | urease | + | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | lipase (C 14) | - | |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21458 | - | + | - | +/- | - | +/- | - | - | - | - | +/- | - | - | - | - | + | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21458 | - | - | - | - | + | + | + | + | + | +/- | - | + | - | +/- | + | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 21458
- sample type: Saharan soil from a palm grove
- geographic location: Ghardaia province, Mzab region, Béni-Isguen, Ntissa palm grove (32°27'52''N, 03°40'19''E, altitude, 468 m)
- country: Algeria
- origin.country: DZA
- continent: Africa
- latitude: 32.4644
- longitude: 3.6719
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Grove (Orchard) |
#Environmental | #Terrestrial | #Desert |
#Environmental | #Terrestrial | #Soil |
#Climate | #Hot | #Arid |
taxonmaps
- @ref: 69479
- File name: preview.99_5405.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_394;97_444;98_4031;99_5405&stattab=map
- Last taxonomy: Actinokineospora
- 16S sequence: KJ504177
- Sequence Identity:
- Total samples: 82
- soil counts: 34
- aquatic counts: 9
- animal counts: 39
Safety information
risk assessment
- @ref: 21458
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21458
- description: Actinokineospora mzabensis strain PAL84 16S ribosomal RNA gene, partial sequence
- accession: KJ504177
- length: 1441
- database: ena
- NCBI tax ID: 1470177
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Actinokineospora spheciospongiae CECT 8578 | GCA_003182415 | scaffold | ncbi | 909613 |
66792 | Actinokineospora mzabensis strain CECT 8578 | 1470177.3 | wgs | patric | 1470177 |
66792 | Actinokineospora mzabensis CECT 8578 | 2770939579 | draft | img | 1470177 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | no | 97.004 | no |
gram-positive | yes | 89.21 | no |
anaerobic | no | 99.255 | no |
halophile | no | 87.245 | no |
spore-forming | yes | 95.996 | no |
glucose-util | yes | 87.456 | no |
aerobic | yes | 91.639 | no |
thermophile | no | 98.901 | yes |
motile | no | 94.006 | no |
glucose-ferment | no | 94.526 | yes |
External links
@ref: 21458
culture collection no.: DSM 45961, CECT 8578, PAL 84
straininfo link
- @ref: 89100
- straininfo: 403018
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 25378048 | Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil. | Aouiche A, Bouras N, Mokrane S, Zitouni A, Schumann P, Sproer C, Sabaou N, Klenk HP | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0328-8 | 2014 | Actinobacteria/*classification/genetics/*isolation & purification/physiology, Africa, Northern, Algeria, Bacterial Typing Techniques, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Temperature, Vitamin K 2/analysis | Genetics |
Phylogeny | 32618556 | Actinokineospora pegani sp. nov., an endophytic actinomycete isolated from the surface-sterilized root of Peganum harmala L. | Lei YJ, Xia ZF, Luo XX, Zhang LL | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004299 | 2020 | Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Peganum/*microbiology, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21458 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45961 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45961) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
89100 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403018.1 |