Strain identifier

BacDive ID: 130568

Type strain: Yes

Species: Pseudomonas asuensis

Strain Designation: CP155-2

Strain history: F. Garcia-Pichel CP 155-2.

NCBI tax ID(s): 1825787 (species)

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General

@ref: 22018

BacDive-ID: 130568

DSM-Number: 17866

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Pseudomonas asuensis CP155-2 is a mesophilic, Gram-negative bacterium that was isolated from biological soil crusts.

NCBI tax id

  • NCBI tax id: 1825787
  • Matching level: species

strain history

@refhistory
22018<- F. Garcia-Pichel, School of Life Sciences, Arizona State University, USA; CP155-2 <- G. S. N. Reddy; CP155-2
67770F. Garcia-Pichel CP 155-2.

doi: 10.13145/bacdive130568.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas asuensis
  • full scientific name: Pseudomonas asuensis Reddy and Garcia-Pichel 2015
  • synonyms

    • @ref: 20215
    • synonym: Chryseomonas asuensis

@ref: 22018

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas asuensis

full scientific name: Pseudomonas asuensis Reddy and Garcia-Pichel 2015

strain designation: CP155-2

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
125438negative97.75
125439negative99.8

Culture and growth conditions

culture medium

  • @ref: 22018
  • name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830c
  • composition: Name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperature
22018positivegrowth30
67770positivegrowth25

Physiology and metabolism

oxygen tolerance

  • @ref: 125439
  • oxygen tolerance: obligate aerobe
  • confidence: 93.3

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 99.6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22018+----+++++++-+++-++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
22018biological soil crustsColorado PlateauUSAUSANorth America38.3831109.889
67770Biological soil crusts from the Colorado PlateauUSAUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_14460.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_224;97_842;98_978;99_14460&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AJ871471
  • Sequence Identity:
  • Total samples: 1365
  • soil counts: 128
  • aquatic counts: 244
  • animal counts: 827
  • plant counts: 166

Safety information

risk assessment

  • @ref: 22018
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Pseudomonas asuensis partial 16S rRNA gene, type strain CP155-2TAJ8714711501nuccore1825787
22018AAJ871471ena

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas asuensis JCM 13501GCA_014646755scaffoldncbi1825787
66792Pseudomonas asuensis strain JCM 135011825787.3wgspatric1825787

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno97.75no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.356no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes89.813no
125438spore-formingspore-formingAbility to form endo- or exosporesno79.36no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno100yes
125438motile2+flagellatedAbility to perform flagellated movementyes86.369no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno99.6
125439BacteriaNetmotilityAbility to perform movementyes85.2
125439BacteriaNetgram_stainReaction to gram-stainingnegative99.8
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe93.3

External links

@ref: 22018

culture collection no.: DSM 17866, ATCC BAA-1264, JCM 13501, KCTC 32484, LMG 28687

straininfo link

  • @ref: 89087
  • straininfo: 397723

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22018Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-17866Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17866)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89087Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397723.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG