Strain identifier

BacDive ID: 130533

Type strain: Yes

Species: Mesorhizobium sangaii

Strain Designation: SCAU7

Strain history: <- HAMBI <- K. Lindström, Univ. Helsinki <- X. Zhang, Sichuan Agricultural Univ., Fac. of Resource and Environmental Scs., China; SCAU7

NCBI tax ID(s): 505389 (species)

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General

@ref: 22041

BacDive-ID: 130533

DSM-Number: 100039

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium sangaii SCAU7 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from effective root nodules of Astragalus luteolus.

NCBI tax id

  • NCBI tax id: 505389
  • Matching level: species

strain history

  • @ref: 22041
  • history: <- HAMBI <- K. Lindström, Univ. Helsinki <- X. Zhang, Sichuan Agricultural Univ., Fac. of Resource and Environmental Scs., China; SCAU7

doi: 10.13145/bacdive130533.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium sangaii
  • full scientific name: Mesorhizobium sangaii Zhou et al. 2013

@ref: 22041

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium sangaii

full scientific name: Mesorhizobium sangaii Zhou et al. 2013

strain designation: SCAU7

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30802negative2 µm0.45 µmrod-shapedyes
69480yes92.817
69480negative99.989

colony morphology

  • @ref: 22041
  • incubation period: 2-3 days

pigmentation

  • @ref: 30802
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 22041
  • name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/98
  • composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
22041positivegrowth30mesophilic
30802positiveoptimum27.5mesophilic

culture pH

@refabilitytypepH
30802positivegrowth06-08
30802positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30802
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30802no
69481no100
69480no99.998

halophily

  • @ref: 30802
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 1 %

observation

  • @ref: 30802
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3080222599arabinose+carbon source
3080218403L-arabitol+carbon source
3080217057cellobiose+carbon source
3080228757fructose+carbon source
3080217234glucose+carbon source
3080217716lactose+carbon source
3080217306maltose+carbon source
3080237684mannose+carbon source
3080228053melibiose+carbon source
3080216634raffinose+carbon source
3080226546rhamnose+carbon source
3080233942ribose+carbon source
3080230911sorbitol+carbon source
3080217992sucrose+carbon source
3080218222xylose+carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22041catalase+1.11.1.6
22041cytochrome-c oxidase+1.9.3.1
30802catalase+1.11.1.6
30802cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
22041--------------------

Isolation, sampling and environmental information

isolation

  • @ref: 22041
  • sample type: effective root nodules of Astragalus luteolus
  • host species: Astragalus luteolus
  • geographic location: Ganzi, Sichuan
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_809.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_643;99_809&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: EU514525
  • Sequence Identity:
  • Total samples: 30688
  • soil counts: 18576
  • aquatic counts: 5652
  • animal counts: 4689
  • plant counts: 1771

Safety information

risk assessment

  • @ref: 22041
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22041
  • description: Mesorhizobium sangaii strain SCAU7 16S ribosomal RNA gene, partial sequence
  • accession: EU514525
  • length: 1356
  • database: ena
  • NCBI tax ID: 505389

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesorhizobium sangaii DSM 100039GCA_014207355scaffoldncbi505389
66792Mesorhizobium sangaii strain DSM 100039505389.5wgspatric505389
66792Mesorhizobium sangaii DSM 1000392861136876draftimg505389

GC content

@refGC-contentmethod
3080258.27
2204158.27high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno87.436no
gram-positiveno97.837yes
anaerobicno99.018yes
aerobicyes91.139no
halophileno93.804yes
spore-formingno94.265yes
glucose-utilyes90.248no
thermophileno99.503yes
glucose-fermentno90.796yes
motileyes89.368no

External links

@ref: 22041

culture collection no.: DSM 100039, ACCC 13218, HAMBI 3318

straininfo link

  • @ref: 89056
  • straininfo: 397932

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23315406Mesorhizobium sangaii sp. nov., isolated from the root nodules of Astragalus luteolus and Astragalus ernestii.Zhou S, Li Q, Jiang H, Lindstrom K, Zhang XInt J Syst Evol Microbiol10.1099/ijs.0.044685-02013Astragalus Plant/*microbiology, Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, SymbiosisGenetics
Phylogeny26610329Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules.De Meyer SE, Tan HW, Andrews M, Heenan PB, Willems AInt J Syst Evol Microbiol10.1099/ijsem.0.0007962015

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22041Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100039Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100039)
30802Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172713328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89056Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397932.1