Strain identifier
BacDive ID: 130532
Type strain: 
Species: Mesorhizobium qingshengii
Strain history: <- LMG <- Xinhua Sui China Agricultural University <- W. Zheng; CCBAU 33460
NCBI tax ID(s): 1165689 (species)
version 8.1 (current version)
General
@ref: 22040
BacDive-ID: 130532
DSM-Number: 29667
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Mesorhizobium qingshengii DSM 29667 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from effective nodule Astragalus sinicus L..
NCBI tax id
- NCBI tax id: 1165689
- Matching level: species
strain history
- @ref: 22040
- history: <- LMG <- Xinhua Sui China Agricultural University <- W. Zheng; CCBAU 33460
doi: 10.13145/bacdive130532.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Phyllobacteriaceae
- genus: Mesorhizobium
- species: Mesorhizobium qingshengii
- full scientific name: Mesorhizobium qingshengii Zheng et al. 2013
@ref: 22040
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Phyllobacteriaceae
genus: Mesorhizobium
species: Mesorhizobium qingshengii
full scientific name: Mesorhizobium qingshengii Zheng et al. 2013
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | confidence |
|---|---|---|---|---|---|
| 30787 | negative | 1.435 µm | 0.45 µm | rod-shaped | |
| 69480 | negative | 99.991 |
colony morphology
- @ref: 22040
- incubation period: 3-7 days
pigmentation
- @ref: 30787
- production: yes
Culture and growth conditions
culture medium
- @ref: 22040
- name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
- growth: yes
- link: https://mediadive.dsmz.de/medium/98
- composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 22040 | positive | growth | 30 | mesophilic |
| 30787 | positive | growth | 10-35 | |
| 30787 | positive | optimum | 28 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30787 | positive | growth | 5.5-9.0 | alkaliphile |
| 30787 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 30787
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30787 | no | |
| 69481 | no | 100 |
| 69480 | no | 99.99 |
halophily
- @ref: 30787
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 1 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30787 | 22599 | arabinose | + | carbon source |
| 30787 | 29016 | arginine | + | carbon source |
| 30787 | 28757 | fructose | + | carbon source |
| 30787 | 28260 | galactose | + | carbon source |
| 30787 | 17234 | glucose | + | carbon source |
| 30787 | 29987 | glutamate | + | carbon source |
| 30787 | 15428 | glycine | + | carbon source |
| 30787 | 17306 | maltose | + | carbon source |
| 30787 | 37684 | mannose | + | carbon source |
| 30787 | 28053 | melibiose | + | carbon source |
| 30787 | 26271 | proline | + | carbon source |
| 30787 | 33942 | ribose | + | carbon source |
| 30787 | 30031 | succinate | + | carbon source |
| 30787 | 17992 | sucrose | + | carbon source |
| 30787 | 26986 | threonine | + | carbon source |
| 30787 | 18222 | xylose | + | carbon source |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 17306 | maltose | + | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 22040 | catalase | + | 1.11.1.6 |
| 22040 | cytochrome-c oxidase | + | 1.9.3.1 |
| 30787 | catalase | + | 1.11.1.6 |
| 30787 | urease | + | 3.5.1.5 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 22040 | - | - | - | - | +/- | - | - | - | - | + | - | - | +/- | + | +/- | - | +/- | +/- | +/- | - |
Isolation, sampling and environmental information
isolation
- @ref: 22040
- sample type: effective nodule Astragalus sinicus L.
- host species: Astragalus sinicus
- geographic location: Jiangxi province, Yuan Zhou district, Yi Chun City
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root nodule |
taxonmaps
- @ref: 69479
- File name: preview.99_809.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_643;99_809&stattab=map
- Last taxonomy: Mesorhizobium
- 16S sequence: JQ339788
- Sequence Identity:
- Total samples: 30688
- soil counts: 18576
- aquatic counts: 5652
- animal counts: 4689
- plant counts: 1771
Safety information
risk assessment
- @ref: 22040
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22040
- description: Mesorhizobium qingshengii strain CCBAU 33460 16S ribosomal RNA gene, partial sequence
- accession: JQ339788
- length: 1308
- database: ena
- NCBI tax ID: 1165689
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mesorhizobium qingshengii CGMCC 1.12097 | GCA_900103325 | scaffold | ncbi | 1165689 |
| 66792 | Mesorhizobium qingshengii strain CGMCC 1.12097 | 1165689.4 | wgs | patric | 1165689 |
| 66792 | Mesorhizobium qingshengii CGMCC 1.12097 | 2596583656 | draft | img | 1165689 |
GC content
- @ref: 30787
- GC-content: 59.52
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 82.189 | no |
| flagellated | no | 88.314 | no |
| gram-positive | no | 97.887 | yes |
| anaerobic | no | 98.63 | no |
| aerobic | yes | 89.189 | yes |
| halophile | no | 94.28 | yes |
| spore-forming | no | 94.911 | yes |
| glucose-ferment | no | 90.93 | yes |
| thermophile | no | 99.39 | no |
| glucose-util | yes | 89.718 | no |
External links
@ref: 22040
culture collection no.: DSM 29667, CCBAU 33460, CGMCC 1.12097, HAMBI 3277, LMG 26793
straininfo link
- @ref: 89055
- straininfo: 389446
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23041644 | Mesorhizobium qingshengii sp. nov., isolated from effective nodules of Astragalus sinicus. | Zheng WT, Li Y, Wang R, Sui XH, Zhang XX, Zhang JJ, Wang ET, Chen WX | Int J Syst Evol Microbiol | 10.1099/ijs.0.044362-0 | 2012 | Astragalus Plant/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal Spacer/genetics, Fatty Acids/analysis, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, Plant Root Nodulation, Plant Roots/*microbiology, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/analysis | Genetics |
| Phylogeny | 26296780 | Mesorhizobium waimense sp. nov. isolated from Sophora longicarinata root nodules and Mesorhizobium cantuariense sp. nov. isolated from Sophora microphylla root nodules. | De Meyer SE, Wee Tan H, Heenan PB, Andrews M, Willems A | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000430 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, New Zealand, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Sophora/*microbiology | Genetics |
| Phylogeny | 32564274 | Mesorhizobium terrae sp. nov., a novel species isolated from soil in Jangsu, Korea. | Jung YJ, Kim HJ, Hur M | Antonie Van Leeuwenhoek | 10.1007/s10482-020-01435-0 | 2020 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, High-Throughput Nucleotide Sequencing, Mesorhizobium/*classification/isolation & purification/*physiology, Molecular Typing, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 35038289 | Aquibium microcysteis gen. nov., sp. nov., isolated from a Microcystis aeruginosa culture and reclassification of Mesorhizobium carbonis as Aquibium carbonis comb. nov. and Mesorhizobium oceanicum as Aquibium oceanicum comb. nov. | Kim M, Kim W, Park W | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005230 | 2022 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Mesorhizobium/classification/isolation & purification, *Microcystis, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 22040 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29667 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29667) | |||
| 30787 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27118 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 68369 | Automatically annotated from API 20NE | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 89055 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID389446.1 |