Strain identifier
BacDive ID: 130530
Type strain:
Species: Mesorhizobium muleiense
Strain history: <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963
NCBI tax ID(s): 1004279 (species)
General
@ref: 22038
BacDive-ID: 130530
DSM-Number: 100038
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Mesorhizobium muleiense DSM 100038 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Cicer arietinum.
NCBI tax id
- NCBI tax id: 1004279
- Matching level: species
strain history
- @ref: 22038
- history: <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963
doi: 10.13145/bacdive130530.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Phyllobacteriaceae
- genus: Mesorhizobium
- species: Mesorhizobium muleiense
- full scientific name: Mesorhizobium muleiense Zhang et al. 2012
@ref: 22038
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Phyllobacteriaceae
genus: Mesorhizobium
species: Mesorhizobium muleiense
full scientific name: Mesorhizobium muleiense Zhang et al. 2012
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
30535 | negative | 1.65 µm | 0.53 µm | rod-shaped | yes | |
69480 | negative | 99.972 |
colony morphology
- @ref: 22038
- incubation period: 3-7 days
pigmentation
- @ref: 30535
- production: yes
Culture and growth conditions
culture medium
- @ref: 22038
- name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
- growth: yes
- link: https://mediadive.dsmz.de/medium/98
- composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
22038 | positive | growth | 30 | mesophilic |
30535 | positive | optimum | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30535 | positive | growth | 06-09 | alkaliphile |
30535 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 30535
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
30535 | no | |
69481 | no | 100 |
69480 | no | 99.973 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30535 | 22599 | arabinose | + | carbon source |
30535 | 28757 | fructose | + | carbon source |
30535 | 28260 | galactose | + | carbon source |
30535 | 17234 | glucose | + | carbon source |
30535 | 17716 | lactose | + | carbon source |
30535 | 37684 | mannose | + | carbon source |
30535 | 26271 | proline | + | carbon source |
30535 | 26546 | rhamnose | + | carbon source |
30535 | 33942 | ribose | + | carbon source |
30535 | 30911 | sorbitol | + | carbon source |
30535 | 17992 | sucrose | + | carbon source |
30535 | 27082 | trehalose | + | carbon source |
30535 | 18222 | xylose | + | carbon source |
68369 | 25115 | malate | - | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
22038 | catalase | + | 1.11.1.6 |
30535 | catalase | + | 1.11.1.6 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22038 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 22038
- sample type: root nodule of Cicer arietinum
- host species: Cicer arietinum
- geographic location: Shuangdamen villages, Qitai county, Xinjiang
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Root nodule |
taxonmaps
- @ref: 69479
- File name: preview.99_1684.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_1684&stattab=map
- Last taxonomy: Mesorhizobium
- 16S sequence: HQ316710
- Sequence Identity:
- Total samples: 4459
- soil counts: 2635
- aquatic counts: 507
- animal counts: 282
- plant counts: 1035
Safety information
risk assessment
- @ref: 22038
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22038
- description: Mesorhizobium muleiense strain CCBAU 83963 16S ribosomal RNA gene, partial sequence
- accession: HQ316710
- length: 1474
- database: ena
- NCBI tax ID: 1004279
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mesorhizobium muleiense CGMCC 1.11022 | GCA_900099905 | scaffold | ncbi | 1004279 |
66792 | Mesorhizobium muleiense strain CGMCC 1.11022 | 1004279.8 | wgs | patric | 1004279 |
66792 | Mesorhizobium muleiense CGMCC 1.11022 | 2675903064 | draft | img | 1004279 |
GC content
@ref | GC-content | method |
---|---|---|
30535 | 61.2 | |
22038 | 61.2 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 76.492 | yes |
flagellated | no | 91.171 | no |
gram-positive | no | 97.832 | yes |
anaerobic | no | 99.357 | yes |
aerobic | yes | 93.815 | yes |
halophile | no | 92.138 | no |
spore-forming | no | 94.957 | yes |
glucose-ferment | no | 90.304 | yes |
thermophile | no | 98.767 | yes |
glucose-util | yes | 90.808 | no |
External links
@ref: 22038
culture collection no.: DSM 100038, CGMCC 1.11022, HAMBI 3264, CCBAU 83963
straininfo link
- @ref: 89053
- straininfo: 401978
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 22228663 | Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L. | Zhang JJ, Liu TY, Chen WF, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX | Int J Syst Evol Microbiol | 10.1099/ijs.0.038265-0 | 2012 | Bacterial Typing Techniques, Base Composition, Cicer/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nitrogen Fixation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA | Genetics |
Genetics | 25123757 | Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils. | Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF, Chen WX | Syst Appl Microbiol | 10.1016/j.syapm.2014.07.004 | 2014 | China, Cicer/*microbiology, DNA, Bacterial, Ecosystem, Mesorhizobium/*genetics/*growth & development, Molecular Sequence Data, *Plant Root Nodulation, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Soil/*chemistry, *Soil Microbiology | Transcriptome |
35793390 | The introduced strain Mesorhizobium ciceri USDA 3378 is more competitive than an indigenous strain in nodulation of chickpea in newly introduced areas of China. | Zhang J, Li S, Wang N, Chen W, Feng X, Jia B, Zhao Y, Yang T, Zong X | Lett Appl Microbiol | 10.1111/lam.13785 | 2022 | United States, *Cicer, United States Department of Agriculture, *Mesorhizobium/genetics, Symbiosis, *Rhizobium, Soil, Chlorophyll |
Reference
@id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
22038 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100038 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100038) | |||
30535 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 26866 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
68369 | Automatically annotated from API 20NE | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
89053 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401978.1 |