Strain identifier

BacDive ID: 130530

Type strain: Yes

Species: Mesorhizobium muleiense

Strain history: <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963

NCBI tax ID(s): 1004279 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22038

BacDive-ID: 130530

DSM-Number: 100038

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium muleiense DSM 100038 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Cicer arietinum.

NCBI tax id

  • NCBI tax id: 1004279
  • Matching level: species

strain history

  • @ref: 22038
  • history: <- HAMBI <- W. F. Chen, China Agric. Univ., College Biol. Sci., Beijing; CCBAU 83963

doi: 10.13145/bacdive130530.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium muleiense
  • full scientific name: Mesorhizobium muleiense Zhang et al. 2012

@ref: 22038

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium muleiense

full scientific name: Mesorhizobium muleiense Zhang et al. 2012

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30535negative1.65 µm0.53 µmrod-shapedyes
69480negative99.972

colony morphology

  • @ref: 22038
  • incubation period: 3-7 days

pigmentation

  • @ref: 30535
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 22038
  • name: RHIZOBIUM MEDIUM (DSMZ Medium 98)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/98
  • composition: Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
22038positivegrowth30mesophilic
30535positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
30535positivegrowth06-09alkaliphile
30535positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30535
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30535no
69481no100
69480no99.973

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3053522599arabinose+carbon source
3053528757fructose+carbon source
3053528260galactose+carbon source
3053517234glucose+carbon source
3053517716lactose+carbon source
3053537684mannose+carbon source
3053526271proline+carbon source
3053526546rhamnose+carbon source
3053533942ribose+carbon source
3053530911sorbitol+carbon source
3053517992sucrose+carbon source
3053527082trehalose+carbon source
3053518222xylose+carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22038catalase+1.11.1.6
30535catalase+1.11.1.6
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
22038--------------------

Isolation, sampling and environmental information

isolation

  • @ref: 22038
  • sample type: root nodule of Cicer arietinum
  • host species: Cicer arietinum
  • geographic location: Shuangdamen villages, Qitai county, Xinjiang
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_1684.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_1684&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: HQ316710
  • Sequence Identity:
  • Total samples: 4459
  • soil counts: 2635
  • aquatic counts: 507
  • animal counts: 282
  • plant counts: 1035

Safety information

risk assessment

  • @ref: 22038
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22038
  • description: Mesorhizobium muleiense strain CCBAU 83963 16S ribosomal RNA gene, partial sequence
  • accession: HQ316710
  • length: 1474
  • database: ena
  • NCBI tax ID: 1004279

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesorhizobium muleiense CGMCC 1.11022GCA_900099905scaffoldncbi1004279
66792Mesorhizobium muleiense strain CGMCC 1.110221004279.8wgspatric1004279
66792Mesorhizobium muleiense CGMCC 1.110222675903064draftimg1004279

GC content

@refGC-contentmethod
3053561.2
2203861.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes76.492yes
flagellatedno91.171no
gram-positiveno97.832yes
anaerobicno99.357yes
aerobicyes93.815yes
halophileno92.138no
spore-formingno94.957yes
glucose-fermentno90.304yes
thermophileno98.767yes
glucose-utilyes90.808no

External links

@ref: 22038

culture collection no.: DSM 100038, CGMCC 1.11022, HAMBI 3264, CCBAU 83963

straininfo link

  • @ref: 89053
  • straininfo: 401978

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22228663Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L.Zhang JJ, Liu TY, Chen WF, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WXInt J Syst Evol Microbiol10.1099/ijs.0.038265-02012Bacterial Typing Techniques, Base Composition, Cicer/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nitrogen Fixation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNAGenetics
Genetics25123757Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils.Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF, Chen WXSyst Appl Microbiol10.1016/j.syapm.2014.07.0042014China, Cicer/*microbiology, DNA, Bacterial, Ecosystem, Mesorhizobium/*genetics/*growth & development, Molecular Sequence Data, *Plant Root Nodulation, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Soil/*chemistry, *Soil MicrobiologyTranscriptome
35793390The introduced strain Mesorhizobium ciceri USDA 3378 is more competitive than an indigenous strain in nodulation of chickpea in newly introduced areas of China.Zhang J, Li S, Wang N, Chen W, Feng X, Jia B, Zhao Y, Yang T, Zong XLett Appl Microbiol10.1111/lam.137852022United States, *Cicer, United States Department of Agriculture, *Mesorhizobium/genetics, Symbiosis, *Rhizobium, Soil, Chlorophyll

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22038Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100038Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100038)
30535Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172686628776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89053Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401978.1