Strain identifier

BacDive ID: 13053

Type strain: Yes

Species: Pseudomonas tolaasii

Strain Designation: B26

Strain history: CIP <- 2000, NCPPB <- D.S. Gandy, strain: B26

NCBI tax ID(s): 564423 (strain), 29442 (species)

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General

@ref: 8059

BacDive-ID: 13053

DSM-Number: 19342

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, plant pathogen

description: Pseudomonas tolaasii B26 is an obligate aerobe, mesophilic, Gram-negative plant pathogen that was isolated from Agaricus bisporus.

NCBI tax id

NCBI tax idMatching level
29442species
564423strain

strain history

@refhistory
8059<- J.-M. Meyer, Insitut Botanique, Strasbourg <- CFBP
67770IAM 15125 <-- CIP 106735 <-- NCPPB 2192 <-- D. S. Gandy B26.
123558CIP <- 2000, NCPPB <- D.S. Gandy, strain: B26

doi: 10.13145/bacdive13053.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas tolaasii
  • full scientific name: Pseudomonas tolaasii Paine 1919 (Approved Lists 1980)

@ref: 8059

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas tolaasii

full scientific name: Pseudomonas tolaasii Paine 1919

strain designation: B26

type strain: yes

Morphology

cell morphology

  • @ref: 123558
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 123558
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8059REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39565MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123558CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123558CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
8059positivegrowth28mesophilic
39565positivegrowth25mesophilic
67770positivegrowth25mesophilic
123558positivegrowth5-30
123558nogrowth37mesophilic
123558nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123558
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123558NaClpositivegrowth0-4 %
123558NaClnogrowth6 %
123558NaClnogrowth8 %
123558NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12355816947citrate-carbon source
12355817632nitrate-reduction
12355816301nitrite-reduction
12355817632nitrate-respiration

antibiotic resistance

  • @ref: 123558
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123558
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123558oxidase+
123558beta-galactosidase-3.2.1.23
123558alcohol dehydrogenase+1.1.1.1
123558gelatinase+/-
123558amylase-
123558DNase-
123558caseinase+3.4.21.50
123558catalase+1.11.1.6
123558tween esterase-
123558lecithinase+
123558lipase+
123558lysine decarboxylase-4.1.1.18
123558ornithine decarboxylase-4.1.1.17
123558protease+
123558tryptophan deaminase-
123558urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123558-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123558+++++--------------+------+++--+++--++--+--++--+++++-+++++-++-+++------+++-+++-++++---++++++++++++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentsampling dategeographic locationisolation date
8059Agaricus bisporusAgaricus bisporusUnited KingdomGBREurope
47800United KingdomGBREurope1965England
67770Agaricus bisporusAgaricus bisporusUnited KingdomGBREurope
123558Agaricus bisporusUnited KingdomGBREurope1965

isolation source categories

  • Cat1: #Host
  • Cat2: #Fungi
  • Cat3: #Mushroom

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
8059yes1Risk group (German classification)
1235581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas tolaasii strain ATCC 33618 16S ribosomal RNA gene, partial sequenceAF0947501459ena29442
20218Pseudomonas tolaasii strain ATCC33618 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF127578539ena29442
20218Pseudomonas tolaasii 16S ribosomal RNA gene, partial sequenceAF2553361492ena29442
20218Pseudomonas tolaasii 16S rRNA geneD840281461ena29442
20218Pseudomonas tolaasii culture-collection CFBP:2068 16S ribosomal RNA gene, partial sequenceHM1902271442ena29442
20218P.tolaasii 16S rRNA geneZ766701491ena29442
8059Pseudomonas tolaasii strain NCPPB 2192 16S ribosomal RNA gene, partial sequenceAF3209881453ena564423

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas tolaasii NCPPB 2192564423.7wgspatric564423
66792Pseudomonas tolaasii NCPPB 2192564423.8wgspatric564423
66792Pseudomonas tolaasii strain CCUG 2336929442.13wgspatric29442
66792Pseudomonas tolaasii NCPPB 21922563366740draftimg564423
66792Pseudomonas tolaasii NCPPB 21922616644975draftimg564423
67770Pseudomonas tolaasii NCPPB 2192GCA_002813445contigncbi564423
67770Pseudomonas tolaasii NCTC11032GCA_900625075contigncbi29442
67770Pseudomonas tolaasii CCUG 23369GCA_008801395contigncbi29442

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno97.876no
anaerobicno98.237no
halophileno88.856no
spore-formingno94.603no
glucose-utilyes95.383no
thermophileno99.913yes
flagellatedyes81.323no
motileyes92.105no
aerobicyes94.515no
glucose-fermentno88.191no

External links

@ref: 8059

culture collection no.: CCUG 23369, CCUG 32782, DSM 19342, ATCC 33618, CFBP 2068, ICMP 2290, LMG 2342, NCPPB 2192, JCM 21583, BCRC 13200, CECT 4472, CIP 106735, IAM 15125, ICMP 12833, ICMP 12843, NBRC 103163, ICMP 12883, PDDCC 2290

straininfo link

  • @ref: 82263
  • straininfo: 4593

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2125501Bacterial siderophores: structures of pyoverdins Pt, siderophores of Pseudomonas tolaasii NCPPB 2192, and pyoverdins Pf, siderophores of Pseudomonas fluorescens CCM 2798. Identification of an unusual natural amino acid.Demange P, Bateman A, Mertz C, Dell A, Piemont Y, Abdallah MABiochemistry10.1021/bi00502a0051990Amino Acid Sequence, Chromatography, High Pressure Liquid, Circular Dichroism, Iron/analysis, Magnetic Resonance Spectroscopy, Molecular Sequence Data, *Oligopeptides, Peptides/*isolation & purification, Peptides, Cyclic/isolation & purification, Pigments, Biological/*chemistry/isolation & purification, Protein Conformation, Pseudomonas/*analysis/growth & development, Pseudomonas fluorescens/*analysis, Spectrometry, Mass, Fast Atom BombardmentPhylogeny
Metabolism3170485Specificity of pyoverdine-mediated iron uptake among fluorescent Pseudomonas strains.Hohnadel D, Meyer JMJ Bacteriol10.1128/jb.170.10.4865-4873.19881988Biological Transport, Cell Membrane/metabolism, Chelating Agents/*metabolism, Iron/*metabolism, Iron Chelating Agents/*metabolism, *Oligopeptides, Pigments, Biological/*metabolism/pharmacology, Pseudomonas/growth & development/*metabolism, Species Specificity, Succinates/metabolismPhylogeny
Enzymology11911616The white-line-in-agar test is not specific for the two cultivated mushroom associated pseudomonads, Pseudomonas tolaasii and Pseudomonas "reactans".Munsch P, Alatossava TMicrobiol Res10.1078/0944-5013-001252002Agaricales/*growth & development, Bacteriological Techniques/methods, Pseudomonas/*growth & development/isolation & purification/pathogenicityPhylogeny
Genetics12002406Evidence for genotypic differences between the two siderovars of Pseudomonas tolaasii, cause of brown blotch disease of the cultivated mushroom Agaricus bisporus.Munsch P, Johnstone K, Alatossava TMicrobiol Res10.1078/0944-5013-001412002Agaricus/*growth & development, Bacterial Proteins/genetics/metabolism, Bacterial Toxins/genetics, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal Spacer/genetics, Depsipeptides, Genotype, Molecular Sequence Data, *Oligopeptides, Pigments, Biological/genetics/metabolism, Polymerase Chain Reaction/methods, Polymorphism, Restriction Fragment Length, Pseudomonas/*genetics/growth & development/pathogenicity, Ribotyping, Sequence Alignment, Sequence Analysis, DNAMetabolism
Metabolism15678564Antimicrobial activity of Streptomyces species against mushroom blotch disease pathogen.Sahin NJ Basic Microbiol10.1002/jobm.2004104272005*Agaricales/growth & development, Agriculture, *Antibiosis, Fermentation, Penicillins/metabolism, Pseudomonas/metabolism/*pathogenicity, Spectrophotometry, Streptomyces/*isolation & purification/metabolism, Virulence, beta-Lactams/chemistry/metabolismEnzymology
Genetics22568623Isolation of new Pseudomonas tolaasii bacteriophages and genomic investigation of the lytic phage BF7.Sajben-Nagy E, Maroti G, Kredics L, Horvath B, Parducz A, Vagvolgyi C, Manczinger LFEMS Microbiol Lett10.1111/j.1574-6968.2012.02592.x2012DNA, Viral/chemistry/genetics, *Genome, Viral, Host Specificity, Microscopy, Electron, Transmission, Molecular Sequence Data, Open Reading Frames, Pleurotus/virology, Podoviridae/genetics/isolation & purification/physiology/ultrastructure, Polymorphism, Restriction Fragment Length, Pseudomonas/*virology, Pseudomonas Phages/*genetics/*isolation & purification/physiology/ultrastructure, Sequence Analysis, DNA, Sequence Homology, Virion/ultrastructurePhylogeny
24031938Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus.Abou-Zeid MABraz J Microbiol10.1590/S1517-8382201200030000412012
35771112First report of Pseudomonas palleroniana causing potato soft rot in China.Zhang Y, Peng S, Ren Y, Yao T, Chu H, Gao Y, Tian XPlant Dis10.1094/PDIS-04-22-0816-PDN2022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8059Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19342)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19342
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39565Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18898
47800Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 23369)https://www.ccug.se/strain?id=23369
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
82263Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4593.1StrainInfo: A central database for resolving microbial strain identifiers
123558Curators of the CIPCollection of Institut Pasteur (CIP 106735)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106735