Strain identifier

BacDive ID: 130496

Type strain: Yes

Species: Nocardiopsis algeriensis

Strain Designation: B32

Strain history: <- N. Sabaou, LPBVB, Kouba, Algeria; B32

NCBI tax ID(s): 1478215 (species)

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General

@ref: 21699

BacDive-ID: 130496

DSM-Number: 45462

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Nocardiopsis algeriensis B32 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from Saharan soil.

NCBI tax id

  • NCBI tax id: 1478215
  • Matching level: species

strain history

  • @ref: 21699
  • history: <- N. Sabaou, LPBVB, Kouba, Algeria; B32

doi: 10.13145/bacdive130496.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Nocardiopsidaceae
  • genus: Nocardiopsis
  • species: Nocardiopsis algeriensis
  • full scientific name: Nocardiopsis algeriensis Bouras et al. 2015

@ref: 21699

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiopsaceae

genus: Nocardiopsis

species: Nocardiopsis algeriensis

full scientific name: Nocardiopsis algeriensis Bouras et al. 2015

strain designation: B32

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.09
69480100positive

colony morphology

@refcolony colormedium used
69397ColourlessISP 5
69397Beige (1001)ISP 2
69397Light ivory (1015)ISP 3
69397Light ivory (1015)ISP 4
69397Light ivory (1015)ISP 7
69397Light ivory (1015)suter with tyrosine
69397Sand yellow (1002)ISP 6
69397Sand yellow (1002)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69397noAerial myceliumISP 2
69397yesAerial myceliumISP 3Cream (9001)
69397yesAerial myceliumISP 4Cream (9001)
69397noAerial myceliumISP 5
69397noAerial myceliumISP 6
69397noAerial myceliumISP 7
69397noAerial myceliumsuter with tyrosine
69397noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69397noMelanin
69397yessoluble pigmentLight ivory (9001)

multimedia

@refmultimedia contentcaptionintellectual property rights
69397DSM_45462_image3.jpegPlates (514, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69397DSM_45462_image4.jpegPlates (514, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21699BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
21699NUTRIMENT AGAR (DSMZ Medium 1259)yeshttps://mediadive.dsmz.de/medium/1259Name: NUTRIMENT AGAR (DSMZ Medium 1259) Composition: Agar 20.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Meat extract 1.0 g/l Distilled water

culture temp

  • @ref: 21699
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes98
69480yes98.674

halophily

  • @ref: 69397
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6939722599arabinose+growth
6939762968cellulose-growth
6939728757fructose+growth
6939717234glucose+growth
6939717268inositol+/-growth
6939737684mannose+growth
6939716634raffinose-growth
6939726546rhamnose-growth
6939717992sucrose+growth
6939718222xylose+growth
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69397+--+--++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69397++/-++/-++/-+/--+/-+/-+/----++/-+--

Isolation, sampling and environmental information

isolation

  • @ref: 21699
  • sample type: Saharan soil
  • geographic location: Touat region, Adrar province, Aougrout (28°69'16''N, 0°31'62''E)
  • country: Algeria
  • origin.country: DZA
  • continent: Africa
  • latitude: 29.1544
  • longitude: 0.5339

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Desert
#Climate#Hot#Arid

Safety information

risk assessment

  • @ref: 21699
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21699
  • description: Nocardiopsis algeriensis strain B32 16S ribosomal RNA gene, partial sequence
  • accession: KJ470139
  • length: 1460
  • database: ena
  • NCBI tax ID: 1478215

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nocardiopsis algeriensis CECT 8712GCA_014203695scaffoldncbi1478215
66792Nocardiopsis algeriensis strain CECT 87121478215.3wgspatric1478215
66792Nocardiopsis algeriensis CECT 87122863327961draftimg1478215

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes98no
flagellatedno97.958no
gram-positiveyes93.354no
anaerobicno99.039no
aerobicyes93.035no
halophileyes52.672no
spore-formingyes88.374no
glucose-utilyes87.343yes
thermophileno94.244yes
motileno92.098no
glucose-fermentno90.366no

External links

@ref: 21699

culture collection no.: DSM 45462, CECT 8712

straininfo link

  • @ref: 89019
  • straininfo: 398986

literature

  • topic: Phylogeny
  • Pubmed-ID: 25380720
  • title: Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil.
  • authors: Bouras N, Meklat A, Zitouni A, Mathieu F, Schumann P, Sproer C, Sabaou N, Klenk HP
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-014-0329-7
  • year: 2014
  • mesh: Actinobacteria/*classification/genetics/*isolation & purification/physiology, Africa, Northern, Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21699Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45462Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45462)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69397Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2045462.pdf
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89019Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398986.1