Strain identifier

BacDive ID: 130494

Type strain: Yes

Species: Nocardioides deserti

Strain Designation: SC8A-24

Strain history: CGMCC 4.7183 <-- L. Tuo; Inst. of Med. Biotechnol., Chin. Acad. Med. Sci., China; SC8A-24.

NCBI tax ID(s): 1588644 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21697

BacDive-ID: 130494

DSM-Number: 26045

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Nocardioides deserti SC8A-24 is a mesophilic bacterium that was isolated from saline–alkaline soil in the rhizosphere of Alhagi sparsifolia.

NCBI tax id

  • NCBI tax id: 1588644
  • Matching level: species

strain history

@refhistory
21697<- Chinese Acad. Med. Sciences & Peking Union Medical College; SC8A-24 <- Y. Dong
67770CGMCC 4.7183 <-- L. Tuo; Inst. of Med. Biotechnol., Chin. Acad. Med. Sci., China; SC8A-24.

doi: 10.13145/bacdive130494.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Nocardioidaceae
  • genus: Nocardioides
  • species: Nocardioides deserti
  • full scientific name: Nocardioides deserti Tuo et al. 2015

@ref: 21697

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardioidaceae

genus: Nocardioides

species: Nocardioides deserti

full scientific name: Nocardioides deserti Tuo et al. 2015

strain designation: SC8A-24

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no90.171
69480100positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21697BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
21697R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21697positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.793

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
21697saline–alkaline soil in the rhizosphere of Alhagi sparsifoliaAlhagi sparsifoliaXinjiang, Taklimakan desert (37° 02' 38'' N 80° 15' 1''E)ChinaCHNAsia37.043980.2503
67770Rhizosphere soil of Alhagis parsifolia from the Taklimakan desertAlhagis parsifoliaXinjiang Province, north-west ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host Body-Site#Plant#Rhizosphere
#Condition#Alkaline
#Condition#Saline

Safety information

risk assessment

  • @ref: 21697
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21697
  • description: Nocardioides deserti strain SC8A-24 16S ribosomal RNA gene, partial sequence
  • accession: KM816582
  • length: 1482
  • database: ena
  • NCBI tax ID: 1588644

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nocardioides deserti CGMCC 4.7183GCA_014646035scaffoldncbi1588644
66792Nocardioides deserti SC8A-24GCA_014266025contigncbi1588644
66792Nocardioides deserti strain CGMCC 4.71831588644.4wgspatric1588644
66792Nocardioides deserti SC8A-242921383071draftimg1588644

GC content

  • @ref: 21697
  • GC-content: 71
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes71no
flagellatedno94.714no
gram-positiveyes89.824no
anaerobicno98.482no
aerobicyes92.968no
halophileno81.583no
spore-formingno88.064no
glucose-utilyes92.236no
thermophileno97.496yes
motileno65.429no
glucose-fermentno88.959no

External links

@ref: 21697

culture collection no.: DSM 26045, CGMCC 4.7183, JCM 33040

straininfo link

  • @ref: 89018
  • straininfo: 404835

literature

  • topic: Phylogeny
  • Pubmed-ID: 25716951
  • title: Nocardioides deserti sp. nov., an actinobacterium isolated from desert soil.
  • authors: Tuo L, Dong YP, Habden X, Liu JM, Guo L, Liu XF, Chen L, Jiang ZK, Liu SW, Zhang YB, Zhang YQ, Sun CH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000147
  • year: 2015
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fabaceae/*microbiology, Fatty Acids/chemistry, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21697Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26045Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26045)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
89018Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404835.1