Strain identifier

BacDive ID: 130451

Type strain: Yes

Species: Acinetobacter harbinensis

Strain Designation: CGMCC1.12528

Strain history: CIP <- 2014, KCTC

NCBI tax ID(s): 1353941 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21452

BacDive-ID: 130451

DSM-Number: 100418

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, oval-shaped

description: Acinetobacter harbinensis CGMCC1.12528 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from river water.

NCBI tax id

  • NCBI tax id: 1353941
  • Matching level: species

strain history

@refhistory
336362014, KCTC
21452<- KCTC <- W. Li; HITLi 7
119838CIP <- 2014, KCTC

doi: 10.13145/bacdive130451.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter harbinensis
  • full scientific name: Acinetobacter harbinensis Li et al. 2014

@ref: 21452

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter harbinensis

full scientific name: Acinetobacter harbinensis Li et al. 2014

strain designation: CGMCC1.12528

type strain: yes

Morphology

cell morphology

  • @ref: 119838
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 21452
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33636MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
21452NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
21452TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
119838CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
21452positivegrowth25mesophilic
33636positivegrowth30mesophilic
63650positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63650
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
21452catalase+1.11.1.6
21452cytochrome-c oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
21452---------------+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21452river waterSonghua River, HarbinChinaCHNAsia
63650River waterHarbin,Songhua RiverChinaCHNAsia
119838Environment, Surface waterSonghua river, ChinaChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

taxonmaps

  • @ref: 69479
  • File name: preview.99_904.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_904&stattab=map
  • Last taxonomy: Acinetobacter harbinensis
  • 16S sequence: KC843488
  • Sequence Identity:
  • Total samples: 331
  • soil counts: 13
  • aquatic counts: 139
  • animal counts: 163
  • plant counts: 16

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
214521Risk group (German classification)
1198381Risk group (French classification)

Sequence information

16S sequences

  • @ref: 21452
  • description: Acinetobacter harbinensis strain HITLi 7 16S ribosomal RNA gene, partial sequence
  • accession: KC843488
  • length: 1328
  • database: ena
  • NCBI tax ID: 1353941

GC content

  • @ref: 21452
  • GC-content: 45.5

External links

@ref: 21452

culture collection no.: DSM 100418, CGMCC 1.12528, HITLi 7, KCTC 32411, CCUG 69700, CIP 110764

straininfo link

@refstraininfo
88978403533
88979408529

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24478215Acinetobacter harbinensis sp. nov., isolated from river water.Li W, Zhang D, Huang X, Qin WInt J Syst Evol Microbiol10.1099/ijs.0.055251-02014Acinetobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNAGenetics
Genetics25744987Draft Genome Sequences of Acinetobacter harbinensis Strain HITLi 7T, Isolated from River Water.Zhang D, Li W, Qin W, Huang X, Gong XGenome Announc10.1128/genomeA.00028-152015Phylogeny
Metabolism28681296Seasonal-related effects on ammonium removal in activated carbon filter biologically enhanced by heterotrophic nitrifying bacteria for drinking water treatment.Qin W, Li WG, Gong XJ, Huang XF, Fan WB, Zhang D, Yao P, Wang XJ, Song YEnviron Sci Pollut Res Int10.1007/s11356-017-9522-32017Acinetobacter/*metabolism, Ammonium Compounds/*analysis, Bioreactors/microbiology, Charcoal/*chemistry, China, Drinking Water/*chemistry/standards, Filtration, Heterotrophic Processes, Seasons, Water Pollutants, Chemical/*analysis, Water Purification/*methods, Water QualityEnzymology
29573607Substrates removal and growth kinetic characteristics of a heterotrophic nitrifying-aerobic denitrifying bacterium, Acinetobacter harbinensis HITLi7(T) at 2 degrees C.Zheng Z, Zhang D, Li W, Qin W, Huang X, Lv LBioresour Technol10.1016/j.biortech.2018.03.0652018*Acinetobacter, Aerobiosis, *Denitrification, Heterotrophic Processes, Nitrification, Nitrogen
31022577Effect of iron and manganese on ammonium removal from micro-polluted source water by immobilized HITLi7(T) at 2 degrees C.Zheng Z, Li W, Zhang D, Qin W, Zhao Y, Lv LBioresour Technol10.1016/j.biortech.2019.1213672019*Ammonium Compounds, Charcoal, Iron, Manganese, *Water Pollutants, Chemical
Phylogeny32725341Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey.Acer O, Guven K, Poli A, Di Donato P, Leone L, Buono L, Guven RG, Nicolaus B, Finore ICurr Microbiol10.1007/s00284-020-02134-92020*Acinetobacter/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, *Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, TurkeyTranscriptome
Phylogeny33196408Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov., isolated from faeces of Equus kiang.Zhu W, Dong K, Yang J, Lu S, Lai XH, Pu J, Jin D, Huang Y, Zhang S, Zhou J, Huang Y, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0045672020Acinetobacter/*classification/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Equidae/*microbiology, Fatty Acids/chemistry, Feces/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tibet, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21452Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100418Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100418)
33636Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30575
63650Curators of the CCUGhttps://www.ccug.se/strain?id=69700Culture Collection University of Gothenburg (CCUG) (CCUG 69700)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88978Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403533.1
88979Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408529.1
119838Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110764Collection of Institut Pasteur (CIP 110764)