Strain identifier

BacDive ID: 130450

Type strain: Yes

Species: Acinetobacter nectaris

Strain history: CIP <- 2012, CECT <- Alvarez-Perez strain: SAP 763.2

NCBI tax ID(s): 1392540 (strain), 1219382 (species)

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General

@ref: 21451

BacDive-ID: 130450

DSM-Number: 29975

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, ovoid-shaped

description: Acinetobacter nectaris CCUG 68431 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Floral nectar of the plant species Muscari comosum.

NCBI tax id

NCBI tax idMatching level
1392540strain
1219382species

strain history

@refhistory
21451<- CECT <- S. Álvarez-Pérez, CSIC, Estación Biológica de Doñana
121255CIP <- 2012, CECT <- Alvarez-Perez strain: SAP 763.2

doi: 10.13145/bacdive130450.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter nectaris
  • full scientific name: Acinetobacter nectaris Álvarez-Pérez et al. 2013

@ref: 21451

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter nectaris

full scientific name: Acinetobacter nectaris Álvarez-Pérez et al. 2013

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30741negative1.5 µm1.5 µmovoid-shapedno
125438negative99.985
125439negative99.5
121255positiveoval-shapedno

colony morphology

@refincubation period
214511-2 days
634851 day

pigmentation

  • @ref: 30741
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42140MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
21451TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
21451NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
121255CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperature
21451positivegrowth30
30741positiveoptimum25-30
42140positivegrowth25
63485positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
30741aerobe
63485aerobe
125439obligate aerobe97.6

spore formation

@refspore formationconfidence
30741no
125438no95.765
125439no98.3

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3074122653asparagine+carbon source
3074135391aspartate+carbon source
3074128757fructose+carbon source
3074129987glutamate+carbon source
3074125115malate+carbon source
3074137684mannose+carbon source
3074126271proline+carbon source
3074130031succinate+carbon source
3074117992sucrose+carbon source
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
21451catalase+1.11.1.6
30741catalase+1.11.1.6
68369urease-3.5.1.5
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
21451--+-----+-----+--+--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
21451Floral nectar of the plant species Muscari comosumMuscari comosumHinojos (Huelva)SpainESPEurope
63485Floral nectar,plant species Muscari comosumHuelvaSpainESPEurope2011
121255Plant, Floral nectar of wild mediterranean insect-pollinated plants. host plant = Muscari comosumHinojos (Huelva, Spain)SpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Flower
#Host Body Product#Plant#Nectar

taxonmaps

  • @ref: 69479
  • File name: preview.99_1188.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_695;97_810;98_938;99_1188&stattab=map
  • Last taxonomy: Acinetobacter nectaris
  • 16S sequence: JQ771132
  • Sequence Identity:
  • Total samples: 5475
  • soil counts: 310
  • aquatic counts: 1357
  • animal counts: 3298
  • plant counts: 510

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
214511Risk group (German classification)
1212551Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
21451Acinetobacter nectaris strain SAP 763.2 16S ribosomal RNA gene, partial sequenceJQ7711321498nuccore1392540
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711291498nuccore1219382
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711301499nuccore1219382
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711311498nuccore1219382
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711331498nuccore1219382
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711341498nuccore1219382
124043Acinetobacter boissieri strain SAP 320.1 16S ribosomal RNA gene, partial sequence.JQ7711351497nuccore1219382

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter nectaris CIP 110549GCA_000488215scaffoldncbi1392540
66792Acinetobacter nectaris CIP 1105491392540.3wgspatric1392540
66792Acinetobacter nectaris CIP 1105492582580904draftimg1392540

GC content

  • @ref: 21451
  • GC-content: 36.6

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno99.985yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.556yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes80.129yes
125438spore-formingspore-formingAbility to form endo- or exosporesno95.765yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno99.5yes
125438motile2+flagellatedAbility to perform flagellated movementno72.37no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.3
125439BacteriaNetmotilityAbility to perform movementyes74
125439BacteriaNetgram_stainReaction to gram-stainingnegative99.5
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe97.6

External links

@ref: 21451

culture collection no.: CCUG 68431, CIP 110549, DSM 29975, CECT 8127, LMG 26958, SAP 763.2

straininfo link

  • @ref: 88977
  • straininfo: 379900

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22904213Acinetobacter nectaris sp. nov. and Acinetobacter boissieri sp. nov., isolated from floral nectar of wild Mediterranean insect-pollinated plants.Alvarez-Perez S, Lievens B, Jacquemyn H, Herrera CMInt J Syst Evol Microbiol10.1099/ijs.0.043489-02012Acinetobacter/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Flowers/microbiology, Insecta, Mediterranean Region, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Plant Nectar/*analysis, Pollination, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny25098562Acinetobacter apis sp. nov., isolated from the intestinal tract of a honey bee, Apis mellifera.Kim PS, Shin NR, Kim JY, Yun JH, Hyun DW, Bae JWJ Microbiol10.1007/s12275-014-4078-02014Acinetobacter/chemistry/*classification/genetics/*isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, Bees/*microbiology, DNA, Bacterial/genetics, Fatty Acids/*analysis, Genotype, Intestines/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phosphatidylethanolamines/isolation & purification, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/isolation & purificationGenetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmedjournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21451Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29975Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29975)
30741Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172707228776041
42140Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8420
63485Curators of the CCUGhttps://www.ccug.se/strain?id=68431Culture Collection University of Gothenburg (CCUG) (CCUG 68431)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa102533211880Nucleic Acids Res. 49: D498-D508 2020
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88977Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID379900.1
121255Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110549Collection of Institut Pasteur (CIP 110549)
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG