Strain identifier

BacDive ID: 130442

Type strain: Yes

Species: Dactylosporangium cerinum

Strain Designation: NEAU-TPG4

Strain history: CGMCC 4.7152 <-- C. Liu; Northeast Agric. Univ., China; NEAU-TPG4.

NCBI tax ID(s): 1434730 (species)

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General

@ref: 21568

BacDive-ID: 130442

DSM-Number: 46712

keywords: 16S sequence, Bacteria, mesophilic

description: Dactylosporangium cerinum NEAU-TPG4 is a mesophilic bacterium that was isolated from rhizosphere soil of Pinus koraiensis Sieb. et Zucc..

NCBI tax id

  • NCBI tax id: 1434730
  • Matching level: species

strain history

@refhistory
21568<- C. Liu, School of Life Science,Harbin,China; NEAU-TPG4
67770CGMCC 4.7152 <-- C. Liu; Northeast Agric. Univ., China; NEAU-TPG4.

doi: 10.13145/bacdive130442.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Dactylosporangium
  • species: Dactylosporangium cerinum
  • full scientific name: Dactylosporangium cerinum Liu et al. 2015

@ref: 21568

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Dactylosporangium

species: Dactylosporangium cerinum

full scientific name: Dactylosporangium cerinum Liu et al. 2015

strain designation: NEAU-TPG4

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69464Daffodil yellow (1007)ISP 2
69464Ivory (1014)ISP 6
69464Ivory (1014), golden yellow (1024)suter with tyrosine
69464Ivory (1014), golden yellow (1024)suter without tyrosine
69464Light ivory (1015)ISP 5
69464Light ivory (1015), ochre yellow (1024)ISP 7
69464Pastel yellow (1034)ISP 3
69464Saffron yellow (1017)ISP 4

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69464noAerial myceliumISP 2
69464noAerial myceliumISP 3
69464noAerial myceliumISP 4
69464noAerial myceliumISP 5
69464noAerial myceliumISP 6
69464noAerial myceliumISP 7
69464noAerial myceliumsuter with tyrosine
69464noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69464noMelanin
69464yessoluble pigmentGolden yellow (1004), beige (1001)

multimedia

  • @ref: 21568
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46712.jpg
  • caption: Medium 554 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21568N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
21568GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21568CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://mediadive.dsmz.de/medium/83Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21568positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

halophily

  • @ref: 69464
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6946422599arabinose+growth
6946462968cellulose+/-growth
6946428757fructose+growth
6946417234glucose+growth
6946417268inositol+/-growth
6946437684mannose+growth
6946416634raffinose+growth
6946426546rhamnose+growth
6946417992sucrose+growth
6946418222xylose+growth
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose-fermentation
6837965327D-xylose+fermentation
6837916899D-mannitol+fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69464-+----++/-+--+-++----

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69464++/-+/--++/--+-++/-+/-+-+++--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
21568rhizosphere soil of Pinus koraiensis Sieb. et Zucc.Pinus koraiensisHeilongjiang Province, Luobei (48°8' N, 130°40'E)ChinaCHNAsia48.1333130.667
67770Rhizosphere soil of Pinus koraiensis Sieb. et Zucc. from LuobeiPinus koraiensisHeilongjiang Province, north ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Tree
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_875.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_521;97_600;98_690;99_875&stattab=map
  • Last taxonomy: Dactylosporangium
  • 16S sequence: KF928748
  • Sequence Identity:
  • Total samples: 1735
  • soil counts: 1581
  • aquatic counts: 58
  • animal counts: 75
  • plant counts: 21

Safety information

risk assessment

  • @ref: 21568
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21568
  • description: Dactylosporangium cerinum strain NEAU-TPG4 16S ribosomal RNA gene, partial sequence
  • accession: KF928748
  • length: 1509
  • database: ena
  • NCBI tax ID: 1434730

GC content

  • @ref: 21568
  • GC-content: 71.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 21568

culture collection no.: DSM 46712, CGMCC 4.7152, JCM 31767

straininfo link

  • @ref: 88971
  • straininfo: 405462

literature

  • topic: Phylogeny
  • Pubmed-ID: 25987396
  • title: Dactylosporangium cerinum sp. nov., a novel actinobacterium isolated from the rhizosphere of Pinus koraiensis Sieb. et Zucc.
  • authors: Liu C, Guan X, Jin P, Li J, Li Y, Li L, Zhou Y, Shen Y, Wang X, Xiang W
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-015-0478-3
  • year: 2015
  • mesh: Carbohydrates/analysis, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Japan, Micromonosporaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Pinus/*microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology, Vitamin K 2/analysis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21568Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46712Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46712)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69464Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2046712.pdf
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88971Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405462.1