Strain identifier
BacDive ID: 130414
Type strain: 
Species: Microbacterium shaanxiense
Strain Designation: CCNWSP5, CCNWSP60
Strain history: CIP <- 2015, S. Peng, Northwest A&F Univ., Yangling, Shaanxi, China: strain CCNWSP60
NCBI tax ID(s): 1507331 (species)
version 8.1 (current version)
General
@ref: 21667
BacDive-ID: 130414
DSM-Number: 28301
keywords: 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped
description: Microbacterium shaanxiense CCNWSP5 is a mesophilic, Gram-positive, rod-shaped bacterium that was isolated from root nodule surface of soybean [Glycine max Merrill] cultivar Zhonghuang 13.
NCBI tax id
- NCBI tax id: 1507331
- Matching level: species
strain history
| @ref | history |
|---|---|
| 41780 | 2015, S. Peng, Northwest A&F Univ., Yangling, Shaanxi, China: strain CCNWSP60 |
| 21667 | <- P. Shi, Northwest A&F Univ., Yangling, China; CCNWSP60 |
| 67770 | S. Peng; Northwest A&F Univ., China; CCNWSP60. |
| 122576 | CIP <- 2015, S. Peng, Northwest A&F Univ., Yangling, Shaanxi, China: strain CCNWSP60 |
doi: 10.13145/bacdive130414.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Microbacteriaceae
- genus: Microbacterium
- species: Microbacterium shaanxiense
- full scientific name: Microbacterium shaanxiense Peng et al. 2015
@ref: 21667
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Microbacteriaceae
genus: Microbacterium
species: Microbacterium shaanxiense
full scientific name: Microbacterium shaanxiense Peng et al. 2015
strain designation: CCNWSP5, CCNWSP60
type strain: yes
Morphology
cell morphology
- @ref: 122576
- gram stain: positive
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 41780 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
| 21667 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water | https://mediadive.dsmz.de/medium/92 |
| 122576 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 41780 | positive | growth | 25 | mesophilic |
| 21667 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: MK-13, MK-12
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent | latitude | longitude | isolation date |
|---|---|---|---|---|---|---|---|---|---|
| 21667 | root nodule surface of soybean [Glycine max (L.) Merrill] cultivar Zhonghuang 13 | Glycine max | Shaanxi Province, Yangling (34° 16' N 108° 4' E) | China | CHN | Asia | 34.2667 | 108.067 | |
| 67770 | Nodule surface of soybean [Glycine max (L.) Merrill] cultivar Zhonghuang 13 | Glycine max | Shaanxi Province | China | CHN | Asia | |||
| 122576 | Nodule surface of soybean cultivar Zhonghuang 13 | Yangling, Shaanxi province | China | CHN | Asia | 2009-06-01 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root nodule |
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 21667 | 1 | Risk group (German classification) |
| 122576 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 21667
- description: Microbacterium shaanxiense strain CCNWSP60 16S ribosomal RNA gene, partial sequence
- accession: KJ735510
- length: 1425
- database: ena
- NCBI tax ID: 1507331
GC content
- @ref: 21667
- GC-content: 67.4
- method: high performance liquid chromatography (HPLC)
External links
@ref: 21667
culture collection no.: DSM 28301, ACCC 19329, JCM 30164, CIP 110973
straininfo link
- @ref: 88946
- straininfo: 398596
literature
- topic: Phylogeny
- Pubmed-ID: 25678679
- title: Microbacterium shaanxiense sp. nov., isolated from the nodule surface of soybean.
- authors: Peng S, Dongying L, Bingxin Y, Mingjun L, Gehong W
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.000116
- year: 2015
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Soybeans/*microbiology, Vitamin K 2/chemistry
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21667 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28301 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28301) | |
| 41780 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/34580 | ||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 88946 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398596.1 | |
| 122576 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110973 | Collection of Institut Pasteur (CIP 110973) |