General

@ref: 21922

BacDive-ID: 130396

DSM-Number: 17370

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina sp. AK-6 is an anaerobe, mesophilic archaeon that was isolated from marine sediment.

NCBI tax id

• NCBI tax id: 2213
• Matching level: species

strain history

• @ref: 21922
• history: <- M. Kendall, Portland State Univ., USA; AK-6 <- Y. Liu {2000}

doi: 10.13145/bacdive130396.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina sp.
• full scientific name: Methanosarcina Kluyver and van Niel 1936 (Approved Lists 1980)

@ref: 21922

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina sp.

full scientific name: Methanosarcina sp.

strain designation: AK-6

type strain: no

Morphology

colony morphology

• @ref: 21922
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 21922
• name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
• growth: yes
• link: https://mediadive.dsmz.de/medium/141c
• composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c) Composition: NaHCO3 4.89237 g/l Trimethylammonium chloride 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

• @ref: 21922
• growth: positive
• type: growth
• temperature: 35
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 21922
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 21922
• sample type: marine sediment
• geographic location: Alaska, Skan Bay
• country: USA
• origin.country: USA
• continent: North America

isolation source categories

Sequence information

16S sequences

• @ref: 21922
• description: Methanosarcina sp. AK-6 16S ribosomal RNA gene, partial sequence
• accession: DQ250385
• length: 1395
• database: ena
• NCBI tax ID: 357353

External links

@ref: 21922

culture collection no.: DSM 17370, OCM 805

straininfo link

• @ref: 88928
• straininfo: 396987

Reference