Strain identifier

BacDive ID: 130382

Type strain: Yes

Species: Limosilactobacillus gorillae

Strain Designation: KZ01

Strain history: K. Ushida KZ01.

NCBI tax ID(s): 1450649 (species)

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General

@ref: 21918

BacDive-ID: 130382

DSM-Number: 28356

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Limosilactobacillus gorillae KZ01 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from faeces of a captive western lowland gorilla .

NCBI tax id

  • NCBI tax id: 1450649
  • Matching level: species

strain history

@refhistory
21918<- K. Ushida, Kyoto Pref. Univ., Japan; KZ01 <- S. Tsuchida
67770K. Ushida KZ01.

doi: 10.13145/bacdive130382.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus gorillae
  • full scientific name: Limosilactobacillus gorillae (Tsuchida et al. 2014) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus gorillae

@ref: 21918

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus gorillae

full scientific name: Limosilactobacillus gorillae (Tsuchida et al. 2014) Zheng et al. 2020

strain designation: KZ01

type strain: yes

Morphology

cell morphology

@refgram stainconfidencemotility
69480positive100
6948096.925no

Culture and growth conditions

culture medium

  • @ref: 21918
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21918positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 21918
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
21918-----+----++++-----------+-++-++---+--------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
21918faeces of a captive western lowland gorilla (Gorilla gorilla gorilla)Gorilla gorilla gorillaKyoto City ZooJapanJPNAsia
67770Feces of a captive western lowland gorilla

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Primates
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 21918
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
21918Lactobacillus gorillae gene for 16S ribosomal RNA, partial sequence, strain: KZ01AB9047161525ena1450649
67770Limosilactobacillus gorillae JCM 19575 gene for 16S rRNA, partial sequenceLC5892191511ena1450649

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus gorillae strain KZ01(T)=JCM19575(T)=DSM28356(T)1450649.3wgspatric1450649
66792Limosilactobacillus gorillae KZ012706794563draftimg1450649
67770Limosilactobacillus gorillae KZ01(T)=JCM19575(T)=DSM28356(T)GCA_001293735contigncbi1450649

GC content

@refGC-contentmethod
2191850.7high performance liquid chromatography (HPLC)
6777048.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.91no
gram-positiveyes95.735no
anaerobicno77.403no
halophileyes91.694no
spore-formingno97.436no
glucose-utilyes87.749no
thermophileno98.465no
aerobicno97.64yes
flagellatedno98.886no
glucose-fermentyes88.481no

External links

@ref: 21918

culture collection no.: DSM 28356, JCM 19575, LMG 30914

straininfo link

  • @ref: 88914
  • straininfo: 400128

literature

  • topic: Phylogeny
  • Pubmed-ID: 25240022
  • title: Lactobacillus gorillae sp. nov., isolated from the faeces of captive and wild western lowland gorillas (Gorilla gorilla gorilla).
  • authors: Tsuchida S, Kitahara M, Nguema PPM, Norimitsu S, Fujita S, Yamagiwa J, Ngomanda A, Ohkuma M, Ushida K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.068429-0
  • year: 2014
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Female, Gabon, Gorilla gorilla/*microbiology, Japan, Lactobacillus/*classification/genetics/isolation & purification, Male, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21918Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28356Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28356)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88914Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400128.1