Strain identifier

BacDive ID: 130359

Type strain: Yes

Species: Anaerostipes rhamnosivorans

Strain Designation: 1y-2, ly-2

Strain history: <- Bui Thi Phuong Nam, Wageningen Univ., Netherlands

NCBI tax ID(s): 1229621 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21475

BacDive-ID: 130359

DSM-Number: 26241

keywords: 16S sequence, Bacteria, obligate anaerobe, spore-forming, mesophilic, rod-shaped

description: Anaerostipes rhamnosivorans 1y-2 is an obligate anaerobe, spore-forming, mesophilic bacterium that was isolated from stool of a 1-year-old healthy infant.

NCBI tax id

  • NCBI tax id: 1229621
  • Matching level: species

strain history

@refhistory
21475<- N. Bui, Wageningen University; 1y-2
67771<- Bui Thi Phuong Nam, Wageningen Univ., Netherlands

doi: 10.13145/bacdive130359.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerostipes
  • species: Anaerostipes rhamnosivorans
  • full scientific name: Anaerostipes rhamnosivorans Bui et al. 2014

@ref: 21475

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerostipes

species: Anaerostipes rhamnosivorans

full scientific name: Anaerostipes rhamnosivorans Bui et al. 2014

strain designation: 1y-2, ly-2

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771rod-shapedno
67771positive

Culture and growth conditions

culture medium

  • @ref: 21475
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
21475positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
21475obligate anaerobe
67771anaerobe

spore formation

  • @ref: 67771
  • type of spore: spore
  • spore formation: yes

compound production

  • @ref: 21475
  • compound: butyrate

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
21475-----------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
21475stool of a 1-year-old healthy infantNetherlandsNLDEurope
67771From stool of a 1-year-old healthy infantNetherlandsNLDEuropeWageningen

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Child

Safety information

risk assessment

  • @ref: 21475
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21475
  • description: Anaerostipes rhamnosivorans strain 1y-2 from Netherlands 16S ribosomal RNA gene, partial sequence
  • accession: JX273468
  • length: 1382
  • database: ena
  • NCBI tax ID: 1229621

GC content

@refGC-contentmethod
2147544.5sequence analysis
6777144.5

External links

@ref: 21475

culture collection no.: DSM 26241, KCTC 15316

straininfo link

  • @ref: 88891
  • straininfo: 401469

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24215821Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium.Bui TPN, de Vos WM, Plugge CMInt J Syst Evol Microbiol10.1099/ijs.0.055061-02013Base Composition, Butyrates/*metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Humans, Infant, Intestines/*microbiology, Molecular Sequence Data, Netherlands, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/geneticsPathogenicity
Phylogeny34491487Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces.Choi SH, Choi JY, Park JE, Kim JS, Kang SW, Lee J, Lee MK, Lee JS, Lee JH, Jung H, Hur TY, Kim HB, Lee JH, Kim JK, Hong Y, Park SHAntonie Van Leeuwenhoek10.1007/s10482-021-01646-z2021Animals, Bacterial Typing Techniques, Clostridiales, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineTranscriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21475Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26241Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26241)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
88891Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401469.1