Strain identifier
BacDive ID: 130359
Type strain:
Species: Anaerostipes rhamnosivorans
Strain Designation: 1y-2, ly-2
Strain history: <- Bui Thi Phuong Nam, Wageningen Univ., Netherlands
NCBI tax ID(s): 1229621 (species)
General
@ref: 21475
BacDive-ID: 130359
DSM-Number: 26241
keywords: 16S sequence, Bacteria, obligate anaerobe, spore-forming, mesophilic, rod-shaped
description: Anaerostipes rhamnosivorans 1y-2 is an obligate anaerobe, spore-forming, mesophilic bacterium that was isolated from stool of a 1-year-old healthy infant.
NCBI tax id
- NCBI tax id: 1229621
- Matching level: species
strain history
@ref | history |
---|---|
21475 | <- N. Bui, Wageningen University; 1y-2 |
67771 | <- Bui Thi Phuong Nam, Wageningen Univ., Netherlands |
doi: 10.13145/bacdive130359.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Lachnospiraceae
- genus: Anaerostipes
- species: Anaerostipes rhamnosivorans
- full scientific name: Anaerostipes rhamnosivorans Bui et al. 2014
@ref: 21475
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Lachnospiraceae
genus: Anaerostipes
species: Anaerostipes rhamnosivorans
full scientific name: Anaerostipes rhamnosivorans Bui et al. 2014
strain designation: 1y-2, ly-2
type strain: yes
Morphology
cell morphology
@ref | cell shape | motility | gram stain |
---|---|---|---|
67771 | rod-shaped | no | |
67771 | positive |
Culture and growth conditions
culture medium
- @ref: 21475
- name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104
- composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21475 | positive | growth | 37 | mesophilic |
67771 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
21475 | obligate anaerobe |
67771 | anaerobe |
spore formation
- @ref: 67771
- type of spore: spore
- spore formation: yes
compound production
- @ref: 21475
- compound: butyrate
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | - | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | - | 3.2.1.23 |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21475 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
21475 | stool of a 1-year-old healthy infant | Netherlands | NLD | Europe | |
67771 | From stool of a 1-year-old healthy infant | Netherlands | NLD | Europe | Wageningen |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | |
#Host | #Human | |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
#Host | #Human | #Child |
Safety information
risk assessment
- @ref: 21475
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21475
- description: Anaerostipes rhamnosivorans strain 1y-2 from Netherlands 16S ribosomal RNA gene, partial sequence
- accession: JX273468
- length: 1382
- database: ena
- NCBI tax ID: 1229621
GC content
@ref | GC-content | method |
---|---|---|
21475 | 44.5 | sequence analysis |
67771 | 44.5 |
External links
@ref: 21475
culture collection no.: DSM 26241, KCTC 15316
straininfo link
- @ref: 88891
- straininfo: 401469
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24215821 | Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium. | Bui TPN, de Vos WM, Plugge CM | Int J Syst Evol Microbiol | 10.1099/ijs.0.055061-0 | 2013 | Base Composition, Butyrates/*metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Humans, Infant, Intestines/*microbiology, Molecular Sequence Data, Netherlands, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics | Pathogenicity |
Phylogeny | 34491487 | Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces. | Choi SH, Choi JY, Park JE, Kim JS, Kang SW, Lee J, Lee MK, Lee JS, Lee JH, Jung H, Hur TY, Kim HB, Lee JH, Kim JK, Hong Y, Park SH | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01646-z | 2021 | Animals, Bacterial Typing Techniques, Clostridiales, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21475 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26241 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26241) | |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||
68380 | Automatically annotated from API rID32A | |||
88891 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401469.1 |