Strain identifier

BacDive ID: 130349

Type strain: Yes

Species: Sporohalobacter salinus

Strain Designation: CEJFT1B

Strain history: J.-L. Cayol CEJFT1B.

NCBI tax ID(s): 1494606 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21799

BacDive-ID: 130349

DSM-Number: 26781

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile

description: Sporohalobacter salinus CEJFT1B is an anaerobe, spore-forming, thermophilic bacterium that was isolated from sediment under the salt crust of a hypersaline lake.

NCBI tax id

  • NCBI tax id: 1494606
  • Matching level: species

strain history

@refhistory
21799<- J.-L. Cayol, IRD, Marseille; CEJFT1B <- M. Ben-Abdallah {2011}
67770J.-L. Cayol CEJFT1B.

doi: 10.13145/bacdive130349.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Halanaerobiales
  • family: Halobacteroidaceae
  • genus: Sporohalobacter
  • species: Sporohalobacter salinus
  • full scientific name: Sporohalobacter salinus Ben Abdallah et al. 2015

@ref: 21799

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Halanaerobiales

family: Halobacteroidaceae

genus: Sporohalobacter

species: Sporohalobacter salinus

full scientific name: Sporohalobacter salinus Ben Abdallah et al. 2015

strain designation: CEJFT1B

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes95.396
6948099.842negative

Culture and growth conditions

culture medium

  • @ref: 21799
  • name: SPOROHALOBACTER SALINUS MEDIUM (DSMZ Medium 1577)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1577
  • composition: Name: SPOROHALOBACTER SALINUS MEDIUM (DSMZ Medium 1577) Composition: MgSO4 5.0 g/l Tryptone 5.0 g/l D-Glucose 4.0 g/l Na2CO3 1.0 g/l NH4Cl 1.0 g/l KCl 0.8 g/l L-Cysteine HCl x H2O 0.5 g/l Yeast extract 0.5 g/l Na-acetate 0.5 g/l K2HPO4 0.5 g/l Na2S x 9 H2O 0.5 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21799positivegrowth40thermophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
21799anaerobe
69480anaerobe100

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 100

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21799sediment under the salt crust of a hypersaline lakeshore of Chott el Jerid lakeTunisiaTUNAfrica
67770Sediment under the salt crust of EI-Jerid hypersaline lake in southern Tunisia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Saline

Safety information

risk assessment

  • @ref: 21799
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21799
  • description: Sporohalobacter salinus strain CEJFT1B 16S ribosomal RNA gene, partial sequence
  • accession: KJ477328
  • length: 1527
  • database: ena
  • NCBI tax ID: 1494606

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sporohalobacter salinus DSM 26781GCA_016908635contigncbi1494606
66792Sporohalobacter salinus strain DSM 267811494606.3wgspatric1494606
66792Sporohalobacter salinus DSM 267812901172808draftimg1494606

GC content

@refGC-contentmethod
2179932.3high performance liquid chromatography (HPLC)
6777032.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes89.457no
gram-positiveno94.103no
anaerobicyes98.817yes
aerobicno96.37yes
halophileno55.719no
spore-formingno54.572no
thermophileyes51.98no
glucose-utilyes86.837no
flagellatedno58.892no
glucose-fermentno62.42no

External links

@ref: 21799

culture collection no.: DSM 26781, JCM 19279

straininfo link

  • @ref: 88881
  • straininfo: 402806

literature

  • topic: Phylogeny
  • Pubmed-ID: 25389147
  • title: Characterization of Sporohalobacter salinus sp. nov., an anaerobic, halophilic, fermentative bacterium isolated from a hypersaline lake.
  • authors: Ben Abdallah M, Karray F, Mhiri N, Cayol JL, Tholozan JL, Alazard D, Sayadi S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.066845-0
  • year: 2014
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Geologic Sediments/microbiology, Gram-Negative Anaerobic Bacteria/*classification/genetics/isolation & purification, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Tunisia
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21799Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26781Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26781)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88881Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402806.1