Strain identifier

BacDive ID: 130328

Type strain: Yes

Species: Psychroflexus salarius

Strain history: K. Y. Jahng MIC1008.

NCBI tax ID(s): 1155689 (species)

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General

@ref: 21747

BacDive-ID: 130328

DSM-Number: 25661

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Psychroflexus salarius DSM 25661 is a mesophilic, Gram-negative bacterium that was isolated from brine from a salt pond.

NCBI tax id

  • NCBI tax id: 1155689
  • Matching level: species

strain history

@refhistory
21747<- J. Chun, Chonbuk National Univ., Dept. of Life Scs., Jeonju-si, Korea; MIC1008
67770K. Y. Jahng MIC1008.

doi: 10.13145/bacdive130328.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Psychroflexus
  • species: Psychroflexus salarius
  • full scientific name: Psychroflexus salarius Chun et al. 2014

@ref: 21747

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Psychroflexus

species: Psychroflexus salarius

full scientific name: Psychroflexus salarius Chun et al. 2014

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.995

Culture and growth conditions

culture medium

  • @ref: 21747
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21747positivegrowth25mesophilic
67770positivegrowth25mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.997

observation

  • @ref: 67770
  • observation: quinones: MK-6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21747brine from a salt pondJeollabuk-do, Buan-gun, Gomso salt panRepublic of KoreaKORAsia
67770Gomso salt pan in Buan CountyRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_4562.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_423;96_2276;97_2759;98_3427;99_4562&stattab=map
  • Last taxonomy: Psychroflexus salarius subclade
  • 16S sequence: JQ390292
  • Sequence Identity:
  • Total samples: 1165
  • soil counts: 96
  • aquatic counts: 1014
  • animal counts: 46
  • plant counts: 9

Safety information

risk assessment

  • @ref: 21747
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21747
  • description: Psychroflexus salarius strain MIC1008 16S ribosomal RNA gene, partial sequence
  • accession: JQ390292
  • length: 1379
  • database: ena
  • NCBI tax ID: 1155689

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Psychroflexus salarius strain DSM 256611155689.4wgspatric1155689
66792Psychroflexus salarius DSM 256612695420935draftimg1155689
67770Psychroflexus salarius DSM 25661GCA_900129035scaffoldncbi1155689

GC content

  • @ref: 21747
  • GC-content: 32.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno87.432no
gram-positiveno98.271no
anaerobicno98.378no
aerobicyes89.793no
halophileyes77.498no
spore-formingno96.389no
flagellatedno95.289no
thermophileno98.311yes
glucose-utilyes84.079no
glucose-fermentno89.31no

External links

@ref: 21747

culture collection no.: DSM 25661, KACC 17063, MIC 1008, JCM 18730

straininfo link

  • @ref: 88861
  • straininfo: 399578

literature

  • topic: Phylogeny
  • Pubmed-ID: 25048209
  • title: Psychroflexus salarius sp. nov., isolated from Gomso salt pan.
  • authors: Chun J, Kang JY, Jahng KY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.065219-0
  • year: 2014
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21747Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-25661Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25661)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88861Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399578.1