Strain identifier

BacDive ID: 130322

Type strain: Yes

Species: Melissococcus plutonius

Strain history: CIP <- 1994, NCFB, Melissococcus pluton <- L. Bailey, "Streptococcus pluton"

NCBI tax ID(s): 940190 (strain), 33970 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21940

BacDive-ID: 130322

DSM-Number: 29964

keywords: genome sequence, Bacteria, anaerobe, spore-forming, mesophilic, animal pathogen

description: Melissococcus plutonius DSM 29964 is an anaerobe, spore-forming, mesophilic animal pathogen that was isolated from honeybee larvae.

NCBI tax id

NCBI tax idMatching level
940190strain
33970species

strain history

@refhistory
21940<- LMG <- ATCC <- NCDO <- L. Bailey; NCDO 2443
121875CIP <- 1994, NCFB, Melissococcus pluton <- L. Bailey, "Streptococcus pluton"

doi: 10.13145/bacdive130322.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Melissococcus
  • species: Melissococcus plutonius
  • full scientific name: Melissococcus plutonius corrig. (ex White 1912) Bailey and Collins 1983
  • synonyms

    @refsynonym
    20215Melisococcus plutonius
    20215Melisococcus plutonis
    20215Melissococcus pluton

@ref: 21940

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Melissococcus

species: Melissococcus plutonius

full scientific name: Melissococcus plutonius (ex White 1912) Bailey and Collins 1983

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.968
69480100positive
121875nopositiverod-shaped

colony morphology

@refincubation period
219402-3 days
121875

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21940MELISSOCOCCUS PLUTONIUS MEDIUM (DSMZ Medium 1582)yeshttps://mediadive.dsmz.de/medium/1582Name: MELISSOCOCCUS PLUTONIUS MEDIUM (DSMZ Medium 1582) Composition: KH2PO4 13.5 g/l Starch 10.0 g/l Glucose 10.0 g/l Yeast extract 10.0 g/l Cysteine hydrochloride 1.0 g/l Distilled water
33056MEDIUM 100 - for Melissococcus plutonyesDistilled water make up to (1000.000 ml);Glucose (10.000 g);Yeast extract (2.500 g);Peptone (2.500 g);Potassium di-hydrogen phosphate (2.500 g);Fructose (5.000 g);Malt extract (5.000 g);Starch maize (2.000 g);Tryptone (2.000 g);Neopeptone (5.000 g);Di Pot
121875CIP Medium 100yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=100
121875CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
121875CIP Medium 93yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=93

culture temp

@refgrowthtypetemperaturerange
21940positivegrowth30mesophilic
33056positivegrowth30mesophilic
121875positivegrowth25-37mesophilic
121875nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
21940anaerobe
121875facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.991

halophily

  • @ref: 121875
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin-hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source
1218754853esculin-hydrolysis
121875606565hippurate-hydrolysis
12187517632nitrate+reduction
12187516301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

antibiotic resistance

  • @ref: 121875
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836735581indoleno
12187535581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6838035581indole-
6836735581indole-
12187515688acetoin-

enzymes

@refvalueactivityec
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367beta-glucosidase-3.2.1.21
68367gelatinase-
68367urease-3.5.1.5
121875oxidase-
121875beta-galactosidase-3.2.1.23
121875alcohol dehydrogenase-1.1.1.1
121875catalase+1.11.1.6
121875gamma-glutamyltransferase-2.3.2.2
121875lysine decarboxylase-4.1.1.18
121875ornithine decarboxylase-4.1.1.17
121875urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121875--++------+---------

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECAT
21940--+------------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
21940---+/----+---------+-++++++++-+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
21940honeybee larvaeUnited KingdomGBREurope
121875Animal, Honey bee larvaeUnited KingdomGBREurope

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
21940yes1Risk group (German classification)
1218751Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Melissococcus plutonius ATCC 35311GCA_000270185chromosomencbi940190
66792Melissococcus plutonius DSM 29964GCA_014202715contigncbi33970
66792Melissococcus plutonius ATCC 35311940190.3completepatric940190
66792Melissococcus plutonius ATCC 35311940190.20plasmidpatric940190
66792Melissococcus plutonius strain DSM 2996433970.54wgspatric33970
66792Melissococcus plutonius ATCC 35311650716050completeimg940190
66792Melissococcus plutonius DSM 299642861600701draftimg33970

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.42no
flagellatedno98.952no
gram-positiveyes95.11no
anaerobicno83.499yes
halophileyes89.274no
spore-formingno92.327no
glucose-utilyes93.136no
aerobicno97.708yes
thermophileno91.862yes
glucose-fermentyes85.18no

External links

@ref: 21940

culture collection no.: DSM 29964, ATCC 35311, LMG 20360, NCDO 2443, CIP 104052, LMG 19520

straininfo link

  • @ref: 88855
  • straininfo: 9302

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics21622755Complete genome sequence of Melissococcus plutonius ATCC 35311.Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama TJ Bacteriol10.1128/JB.05151-112011Animals, Base Sequence, Bees/*microbiology, Enterococcaceae/*genetics/isolation & purification, *Genome, Bacterial, Molecular Sequence DataEnzymology
35622764Antimicrobial Activity from Putative Probiotic Lactic Acid Bacteria for the Biological Control of American and European Foulbrood Diseases.Iorizzo M, Ganassi S, Albanese G, Letizia F, Testa B, Tedino C, Petrarca S, Mutinelli F, Mazzeo A, De Cristofaro AVet Sci10.3390/vetsci90502362022

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21940Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29964Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29964)
33056Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15917
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88855Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID9302.1
121875Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104052Collection of Institut Pasteur (CIP 104052)