Strain identifier
BacDive ID: 130322
Type strain:
Species: Melissococcus plutonius
Strain history: CIP <- 1994, NCFB, Melissococcus pluton <- L. Bailey, "Streptococcus pluton"
NCBI tax ID(s): 940190 (strain), 33970 (species)
General
@ref: 21940
BacDive-ID: 130322
DSM-Number: 29964
keywords: genome sequence, Bacteria, anaerobe, spore-forming, mesophilic, animal pathogen
description: Melissococcus plutonius DSM 29964 is an anaerobe, spore-forming, mesophilic animal pathogen that was isolated from honeybee larvae.
NCBI tax id
NCBI tax id | Matching level |
---|---|
940190 | strain |
33970 | species |
strain history
@ref | history |
---|---|
21940 | <- LMG <- ATCC <- NCDO <- L. Bailey; NCDO 2443 |
121875 | CIP <- 1994, NCFB, Melissococcus pluton <- L. Bailey, "Streptococcus pluton" |
doi: 10.13145/bacdive130322.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Enterococcaceae
- genus: Melissococcus
- species: Melissococcus plutonius
- full scientific name: Melissococcus plutonius corrig. (ex White 1912) Bailey and Collins 1983
synonyms
@ref synonym 20215 Melisococcus plutonius 20215 Melisococcus plutonis 20215 Melissococcus pluton
@ref: 21940
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Enterococcaceae
genus: Melissococcus
species: Melissococcus plutonius
full scientific name: Melissococcus plutonius (ex White 1912) Bailey and Collins 1983
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 95.968 | ||
69480 | 100 | positive | ||
121875 | no | positive | rod-shaped |
colony morphology
@ref | incubation period |
---|---|
21940 | 2-3 days |
121875 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
21940 | MELISSOCOCCUS PLUTONIUS MEDIUM (DSMZ Medium 1582) | yes | https://mediadive.dsmz.de/medium/1582 | Name: MELISSOCOCCUS PLUTONIUS MEDIUM (DSMZ Medium 1582) Composition: KH2PO4 13.5 g/l Starch 10.0 g/l Glucose 10.0 g/l Yeast extract 10.0 g/l Cysteine hydrochloride 1.0 g/l Distilled water |
33056 | MEDIUM 100 - for Melissococcus pluton | yes | Distilled water make up to (1000.000 ml);Glucose (10.000 g);Yeast extract (2.500 g);Peptone (2.500 g);Potassium di-hydrogen phosphate (2.500 g);Fructose (5.000 g);Malt extract (5.000 g);Starch maize (2.000 g);Tryptone (2.000 g);Neopeptone (5.000 g);Di Pot | |
121875 | CIP Medium 100 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=100 | |
121875 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
121875 | CIP Medium 93 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=93 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
21940 | positive | growth | 30 | mesophilic |
33056 | positive | growth | 30 | mesophilic |
121875 | positive | growth | 25-37 | mesophilic |
121875 | no | growth | 10 | psychrophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
21940 | anaerobe |
121875 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | no | 99.991 |
halophily
- @ref: 121875
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 6.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68367 | 27082 | trehalose | - | builds acid from |
68367 | 62345 | L-rhamnose | - | builds acid from |
68367 | 30911 | sorbitol | - | builds acid from |
68367 | 16634 | raffinose | - | builds acid from |
68367 | 6731 | melezitose | - | builds acid from |
68367 | 16024 | D-mannose | - | builds acid from |
68367 | 17057 | cellobiose | - | builds acid from |
68367 | 17754 | glycerol | - | builds acid from |
68367 | 4853 | esculin | - | hydrolysis |
68367 | 5291 | gelatin | - | hydrolysis |
68367 | 30849 | L-arabinose | - | builds acid from |
68367 | 65327 | D-xylose | - | builds acid from |
68367 | 17814 | salicin | - | builds acid from |
68367 | 17306 | maltose | - | builds acid from |
68367 | 17992 | sucrose | - | builds acid from |
68367 | 17716 | lactose | - | builds acid from |
68367 | 16899 | D-mannitol | - | builds acid from |
68367 | 17634 | D-glucose | + | builds acid from |
68367 | 16199 | urea | - | hydrolysis |
68367 | 27897 | tryptophan | - | energy source |
121875 | 4853 | esculin | - | hydrolysis |
121875 | 606565 | hippurate | - | hydrolysis |
121875 | 17632 | nitrate | + | reduction |
121875 | 16301 | nitrite | - | reduction |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
antibiotic resistance
- @ref: 121875
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68367 | 35581 | indole | no |
121875 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test | voges-proskauer-test |
---|---|---|---|---|
68380 | 35581 | indole | - | |
68367 | 35581 | indole | - | |
121875 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | + | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | + | |
68380 | glycin arylamidase | + | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | + | |
68380 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68380 | leucine arylamidase | + | 3.4.11.1 |
68380 | phenylalanine arylamidase | + | |
68380 | leucyl glycin arylamidase | + | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | + | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | + | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | - | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
68367 | catalase | - | 1.11.1.6 |
68367 | beta-glucosidase | - | 3.2.1.21 |
68367 | gelatinase | - | |
68367 | urease | - | 3.5.1.5 |
121875 | oxidase | - | |
121875 | beta-galactosidase | - | 3.2.1.23 |
121875 | alcohol dehydrogenase | - | 1.1.1.1 |
121875 | catalase | + | 1.11.1.6 |
121875 | gamma-glutamyltransferase | - | 2.3.2.2 |
121875 | lysine decarboxylase | - | 4.1.1.18 |
121875 | ornithine decarboxylase | - | 4.1.1.17 |
121875 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121875 | - | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API 20A
@ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE | CAT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21940 | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21940 | - | - | - | +/- | - | - | - | + | - | - | - | - | - | - | - | - | - | + | - | + | + | + | + | + | + | + | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
21940 | honeybee larvae | United Kingdom | GBR | Europe |
121875 | Animal, Honey bee larvae | United Kingdom | GBR | Europe |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
21940 | yes | 1 | Risk group (German classification) |
121875 | 1 | Risk group (French classification) |
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Melissococcus plutonius ATCC 35311 | GCA_000270185 | chromosome | ncbi | 940190 |
66792 | Melissococcus plutonius DSM 29964 | GCA_014202715 | contig | ncbi | 33970 |
66792 | Melissococcus plutonius ATCC 35311 | 940190.3 | complete | patric | 940190 |
66792 | Melissococcus plutonius ATCC 35311 | 940190.20 | plasmid | patric | 940190 |
66792 | Melissococcus plutonius strain DSM 29964 | 33970.54 | wgs | patric | 33970 |
66792 | Melissococcus plutonius ATCC 35311 | 650716050 | complete | img | 940190 |
66792 | Melissococcus plutonius DSM 29964 | 2861600701 | draft | img | 33970 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | no | 94.42 | no |
flagellated | no | 98.952 | no |
gram-positive | yes | 95.11 | no |
anaerobic | no | 83.499 | yes |
halophile | yes | 89.274 | no |
spore-forming | no | 92.327 | no |
glucose-util | yes | 93.136 | no |
aerobic | no | 97.708 | yes |
thermophile | no | 91.862 | yes |
glucose-ferment | yes | 85.18 | no |
External links
@ref: 21940
culture collection no.: DSM 29964, ATCC 35311, LMG 20360, NCDO 2443, CIP 104052, LMG 19520
straininfo link
- @ref: 88855
- straininfo: 9302
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Genetics | 21622755 | Complete genome sequence of Melissococcus plutonius ATCC 35311. | Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T | J Bacteriol | 10.1128/JB.05151-11 | 2011 | Animals, Base Sequence, Bees/*microbiology, Enterococcaceae/*genetics/isolation & purification, *Genome, Bacterial, Molecular Sequence Data | Enzymology |
35622764 | Antimicrobial Activity from Putative Probiotic Lactic Acid Bacteria for the Biological Control of American and European Foulbrood Diseases. | Iorizzo M, Ganassi S, Albanese G, Letizia F, Testa B, Tedino C, Petrarca S, Mutinelli F, Mazzeo A, De Cristofaro A | Vet Sci | 10.3390/vetsci9050236 | 2022 |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
21940 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29964 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29964) | |||
33056 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15917 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68367 | Automatically annotated from API 20A | |||||
68380 | Automatically annotated from API rID32A | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
88855 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID9302.1 | |||
121875 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104052 | Collection of Institut Pasteur (CIP 104052) |