Strain identifier
BacDive ID: 130261
Type strain: 
Species: Ottowia beijingensis
Strain Designation: GCS-AN-3
Strain history: <- LMG <- Q. Lai, Key Lab. Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, China
NCBI tax ID(s): 1207057 (species)
version 8.1 (current version)
General
@ref: 21712
BacDive-ID: 130261
DSM-Number: 100246
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative
description: Ottowia beijingensis GCS-AN-3 is a mesophilic, Gram-negative bacterium that was isolated from coking wastewater activated sludge.
NCBI tax id
- NCBI tax id: 1207057
- Matching level: species
strain history
- @ref: 21712
- history: <- LMG <- Q. Lai, Key Lab. Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, China
doi: 10.13145/bacdive130261.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Comamonadaceae
- genus: Ottowia
- species: Ottowia beijingensis
- full scientific name: Ottowia beijingensis Cao et al. 2014
@ref: 21712
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Comamonadaceae
genus: Ottowia
species: Ottowia beijingensis
full scientific name: Ottowia beijingensis Cao et al. 2014
strain designation: GCS-AN-3
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.999
Culture and growth conditions
culture medium
- @ref: 21712
- name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535
- composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
culture temp
- @ref: 21712
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.965 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | + | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | + | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | + | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21712 | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | + | + | - | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 21712
- sample type: coking wastewater activated sludge
- geographic location: Beijing
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Waste | #Activated sludge |
| #Engineered | #Waste | #Wastewater |
taxonmaps
- @ref: 69479
- File name: preview.99_29533.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_7559;97_9076;98_22016;99_29533&stattab=map
- Last taxonomy: Ottowia
- 16S sequence: KC755038
- Sequence Identity:
- Total samples: 386
- soil counts: 48
- aquatic counts: 210
- animal counts: 120
- plant counts: 8
Safety information
risk assessment
- @ref: 21712
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21712
- description: Ottowia beijingensis strain GCS-AN-3 16S ribosomal RNA gene, partial sequence
- accession: KC755038
- length: 1492
- database: ena
- NCBI tax ID: 1207057
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Ottowia beijingensis GCS-AN-3 | GCA_013423955 | contig | ncbi | 1207057 |
| 66792 | Ottowia beijingensis strain GCS-AN-3 | 1207057.3 | wgs | patric | 1207057 |
GC content
- @ref: 21712
- GC-content: 67.6
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 79.732 | no |
| gram-positive | no | 98.68 | no |
| anaerobic | no | 97.26 | no |
| aerobic | yes | 87.295 | no |
| halophile | no | 93.675 | no |
| spore-forming | no | 95.609 | no |
| thermophile | no | 93.265 | no |
| glucose-util | no | 88.211 | yes |
| flagellated | no | 93.263 | no |
| glucose-ferment | no | 91.317 | yes |
External links
@ref: 21712
culture collection no.: DSM 100246, CGMCC 1.12324, LMG 27179, MCCC 1A01410
straininfo link
- @ref: 88800
- straininfo: 381214
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24425809 | Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia. | Cao J, Lai Q, Liu Y, Li G, Shao Z | Int J Syst Evol Microbiol | 10.1099/ijs.0.054015-0 | 2013 | Bacterial Typing Techniques, Base Composition, China, Coke/*microbiology, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry, Waste Water/microbiology | Genetics |
| Phylogeny | 30102146 | Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage. | Heo J, Cho H, Hong SB, Kim JS, Kwon SW, Kim SJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002935 | 2018 | Bacterial Typing Techniques, Base Composition, Beverages/*microbiology, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Oryza, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Ubiquinone/chemistry | Biotechnology |
| Phylogeny | 30222093 | Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue). | Yi KJ, Im WT, Kim DW, Kim SK | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003009 | 2018 | Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soy Foods/*microbiology, Ubiquinone/chemistry | Biotechnology |
| Phylogeny | 31147966 | Ottowia flava sp. nov., isolated from fish intestines. | Shi SB, Li GD, Yang LF, Liu C, Jiang MG, Li QY, Wu JF, Zhang K, Jiang LQ, Shen NK, Jiang CL, Jiang Y | Antonie Van Leeuwenhoek | 10.1007/s10482-019-01284-6 | 2019 | Aerobiosis, Animals, Aquatic Organisms/microbiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Comamonadaceae/*classification/genetics/*isolation & purification/physiology, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fishes/*microbiology, Hydrogen-Ion Concentration, Intestines/*microbiology, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature | Transcriptome |
| Phylogeny | 35604929 | Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis). | Busse HJ, Kampfer P, Szostak MP, Spergser J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005348 | 2022 | Animals, Bacterial Typing Techniques, Base Composition, Cloaca, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Turtles | Transcriptome |
| Phylogeny | 36044996 | Ottowia massiliensis sp. nov., a new bacterium isolated from a fresh, healthy human fecal sample. | Maaloum M, Lo CI, Ndongo S, Meng MM, Saile R, Alibar S, Raoult D, Fournier PE | FEMS Microbiol Lett | 10.1093/femsle/fnac086 | 2022 | Base Composition, Catalase/genetics, DNA, Bacterial/genetics, Feces/microbiology, Humans, Phylogeny, *RNA, Ribosomal, 16S/genetics | Pathogenicity |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21712 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100246 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100246) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68369 | Automatically annotated from API 20NE | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 88800 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID381214.1 |