Strain identifier

BacDive ID: 130241

Type strain: Yes

Species: Pseudactinotalea suaedae

Strain history: <- Wenjun Li, China

NCBI tax ID(s): 1524924 (species)

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General

@ref: 22046

BacDive-ID: 130241

DSM-Number: 28005

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Pseudactinotalea suaedae DSM 28005 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from surface-sterilized healthy plant material of the halophyte Suaeda physophora.

NCBI tax id

  • NCBI tax id: 1524924
  • Matching level: species

strain history

@refhistory
22046<- W.-J. Li, Xinjiang Inst. of Ecol. and Geography (XIEG), Chin. Acad. of Scs., Urumchi, China; EGI 60002 <- L. Li
67770W.-J. Li EGI 60002.
67771<- Wenjun Li, China

doi: 10.13145/bacdive130241.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Pseudactinotalea
  • species: Pseudactinotalea suaedae
  • full scientific name: Pseudactinotalea suaedae (Zhao et al. 2015) Cho et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Actinotalea suaedae

@ref: 22046

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Pseudactinotalea

species: Pseudactinotalea suaedae

full scientific name: Pseudactinotalea suaedae (Zhao et al. 2015) Cho et al. 2017

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
25230negativerod-shapedno
69480no94.132
69480positive100

colony morphology

  • @ref: 25230
  • colony color: yellow
  • colony shape: circular
  • incubation period: 4 days
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25230Trypticase Soy Agar (TSA)yes
25230Reasoner's 2A agar (R2A)yes
25230LB (Luria-Bertani) MEDIUMyes
25230MacConkey agarno
22046TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25230positivegrowth15-35
25230positiveoptimum28mesophilic
22046positivegrowth28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepH
25230positivegrowth6.0-8.0
25230positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25230aerobe
67771aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.967

halophily

@refsaltgrowthtested relationconcentration
25230NaClpositivegrowth0.0-5.0 %(w/v)
25230NaCloptimum3 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25230161933-hydroxybenzoate-assimilation
25230370543-hydroxybutyrate-assimilation
25230178794-hydroxybenzoate-assimilation
2523017128adipate-assimilation
2523017108D-arabinose-builds acid from
2523017924D-sorbitol-assimilation
2523027689decanoate-assimilation
2523016991dna-hydrolysis
2523017234glucose-fermentation
2523028087glycogen-assimilation
2523017268myo-inositol-assimilation
2523017240itaconate-assimilation
2523016977L-alanine-assimilation
2523015971L-histidine-assimilation
2523017203L-proline-assimilation
2523017115L-serine-assimilation
2523017266L-sorbose-builds acid from
2523024996lactate-assimilation
2523017716lactose-builds acid from
2523025115malate-assimilation
2523018401phenylacetate-assimilation
25230potassium 5-dehydro-D-gluconate-assimilation
2523017272propionate-assimilation
2523032954sodium acetate-assimilation
2523053258sodium citrate-assimilation
2523062983sodium malonate-assimilation
252309300suberic acid-assimilation
2523053426tween 80-hydrolysis
2523031011valerate-assimilation
2523062968cellulose+hydrolysis
2523015824D-fructose+builds acid from
2523012936D-galactose+builds acid from
2523017634D-glucose+builds acid from
2523016899D-mannitol+builds acid from
2523016024D-mannose+builds acid from
2523016988D-ribose+assimilation
2523065327D-xylose+builds acid from
2523023652dextrin+hydrolysis
252304853esculin+hydrolysis
252305291gelatin+hydrolysis
2523030849L-arabinose+builds acid from
2523018287L-fucose+assimilation
2523062345L-rhamnose+assimilation
2523017306maltose+builds acid from
2523028053melibiose+assimilation
25230506227N-acetylglucosamine+assimilation
2523017632nitrate+reduction
2523017309pectin+hydrolysis
25230potassium 2-dehydro-D-gluconate+assimilation
2523032032potassium gluconate+assimilation
2523017814salicin+assimilation
2523028017starch+hydrolysis
2523017992sucrose+builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
252309332sulfamethoxazoleyesyes1.25 µg
25230100246norfloxacinyesyes10 µg
252303745clindamycinyesyes2 µg
25230474053cefazolinyesyes30 µg
2523048923erythromycinyesyes15 µg
252307731ofloxacinyesyes5 µg
25230100241ciprofloxacinyesyes5 µg
2523028971ampicillinyesyes10 µg
2523017334penicillinyesyes10 Unit
2523017698chloramphenicolyesyes30 µg
252302637amikacinyesyes30 µg
252308309polymyxin byesyes300 Unit
2523017833gentamicinyesyes10 µg
252308232piperacillinyesyes100 µg
252303515cefuroximeyesyes30 µg
2523028001vancomycinyesyes30 µg
2523029007ceftriaxoneyesyes30 µg
252303493cefoperazoneyesyes75 µg

metabolite production

  • @ref: 25230
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
25230acid phosphatase-3.1.3.2
25230alkaline phosphatase-3.1.3.1
25230alpha-chymotrypsin-3.4.21.1
25230alpha-fucosidase-3.2.1.51
25230alpha-galactosidase-3.2.1.22
25230alpha-glucosidase+3.2.1.20
25230alpha-mannosidase+3.2.1.24
25230arginine dihydrolase+3.5.3.6
25230beta-galactosidase+3.2.1.23
25230beta-glucosidase+3.2.1.21
25230beta-glucuronidase-3.2.1.31
25230cystine arylamidase-3.4.11.3
25230cytochrome oxidase-1.9.3.1
25230esterase (C 4)+
25230esterase Lipase (C 8)+
25230leucine arylamidase+3.4.11.1
25230lipase (C 14)-
25230N-acetyl-beta-glucosaminidase+3.2.1.52
25230naphthol-AS-BI-phosphohydrolase+
25230trypsin-3.4.21.4
25230urease-3.5.1.5
25230valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    25230C15:0 anteiso66.3
    25230C15:1 anteiso A3.1
    25230C17:0 anteiso1.7
    25230C14:06.6
    25230C16:02
    25230C14:0 iso7.5
    25230C15:0 iso7.2
    25230Unknown219
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • software version: Sherlock 5.0
  • library/peak naming table: TSBA 50
  • system: MIS MIDI
  • method/protocol: Sasser 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
22046surface-sterilized healthy plant material (stems and roots) of the halophyte Suaeda physophoraSuaeda physophoraXinjiang, near the Fukang Field Research Station of the Chinese Academy of Sciences (44°17' N 87°55' E)ChinaCHNAsia44.283387.9167
25230Suaeda physophora grown in saline areasFukang City, Xinjiang Province, Northwest ChinaChinaCHNAsia
67770Halophyte Suaeda physophora grown on saline areas of Fukang CitySuaeda physophoraXinjiang Province, northwest ChinaChinaCHNAsia
67771From GlycyrrhizaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root (Rhizome)
#Host Body-Site#Plant#Stem (Branch)
#Host Body-Site#Plant#Sterilized plant part

taxonmaps

  • @ref: 69479
  • File name: preview.99_59109.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2302;97_32659;98_41886;99_59109&stattab=map
  • Last taxonomy: Cellulomonadaceae
  • 16S sequence: KJ769194
  • Sequence Identity:
  • Total samples: 1572
  • soil counts: 388
  • aquatic counts: 398
  • animal counts: 641
  • plant counts: 145

Safety information

risk assessment

  • @ref: 22046
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22046
  • description: Actinotalea suaedae strain EGI 60002 16S ribosomal RNA gene, partial sequence
  • accession: KJ769194
  • length: 1563
  • database: ena
  • NCBI tax ID: 1524924

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudactinotalea suaedae strain KACC 178391524924.3wgspatric1524924
66792Pseudactinotalea suaedae KACC 178392887398948draftimg1524924
67770Pseudactinotalea suaedae KACC 17839GCA_009733815contigncbi1524924

GC content

@refGC-contentmethod
2523072.3
2204672.3high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno77no
motileno92.314yes
gram-positiveyes78.837yes
anaerobicno99.467yes
aerobicyes94.099yes
halophileno90.233no
spore-formingno91.78no
glucose-utilyes87.231no
flagellatedno98.811no
thermophileno98.572yes
glucose-fermentno88.456yes

External links

@ref: 22046

culture collection no.: DSM 28005, JCM 19624, KACC 17839, KCTC 29256, EGI 60002

straininfo link

  • @ref: 88783
  • straininfo: 396670

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25307231Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China.Zhao S, Li L, Li SH, Wang HF, Hozzein WN, Zhang YG, Wadaan MA, Li WJ, Tian CYAntonie Van Leeuwenhoek10.1007/s10482-014-0297-y2014Actinobacteria/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, Chenopodiaceae/*microbiology, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Salt-Tolerant Plants/microbiology, Sequence Analysis, DNAGenetics
Phylogeny27902298Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov.Cho H, Hamada M, Ahn JH, Weon HY, Joa JH, Suzuki KI, Kwon SW, Kim SJInt J Syst Evol Microbiol10.1099/ijsem.0.0017012017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny28036250Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor.Jin L, Ko SR, Lee CS, Ahn CY, Lee JS, Lee KC, Oh HM, Lee HGInt J Syst Evol Microbiol10.1099/ijsem.0.0017692017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Biofilms, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22046Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28005Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28005)
25230H. H. Cho, M.,Ahn, J. H.,Weon, H. Y.,Joa, J. H.,Suzuki, K. I.,Kwon, S. W.,Kim, S. J.Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov10.1099/ijsem.0.001701IJSEM 67: 704-709 201727902298
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
88783Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396670.1