Strain identifier

BacDive ID: 130193

Type strain: Yes

Species: Bacillus pakistanensis

Strain history: <- I Ahmed, NCCP, Pakistan

NCBI tax ID(s): 992288 (species)

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General

@ref: 21524

BacDive-ID: 130193

DSM-Number: 24834

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, rod-shaped

description: Bacillus pakistanensis DSM 24834 is an aerobe, spore-forming, mesophilic bacterium that was isolated from salt mine.

NCBI tax id

  • NCBI tax id: 992288
  • Matching level: species

strain history

@refhistory
21524<- I. Ahmed, National Institute for Genomics and Advanced Biotechnology, Pakistan; NCCP-168 <- A. Roohi and I. Ahmed
67770I. Ahmed NCCP-168.
67771<- I Ahmed, NCCP, Pakistan

doi: 10.13145/bacdive130193.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus pakistanensis
  • full scientific name: Bacillus pakistanensis Roohi et al. 2014

@ref: 21524

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus pakistanensis

full scientific name: Bacillus pakistanensis Roohi et al. 2014

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stainconfidence
67771rod-shapedno
67771positive
69480yes98.065
69480positive100

Culture and growth conditions

culture medium

  • @ref: 21524
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21524positivegrowth30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30-35mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

observation

@refobservation
67770quinones: MK-7, MK-8
67771quinones: MK-7

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21524salt mineKhyber Pakhtunkhwa Province, Karak region, Bahadur Khel salt minesPakistanPAKAsia
67770Salt mine sample collected from the Karak regionKhyber Pakhtunkhwa ProvincePakistanPAKAsia
67771From salt minethe Karak region of the Khyber Pakhtunkhwa ProvincePakistanPAKAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Condition#Saline

Safety information

risk assessment

  • @ref: 21524
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21524
  • description: Bacillus pakistanensis gene for 16S rRNA, partial sequence
  • accession: AB618147
  • length: 1411
  • database: ena
  • NCBI tax ID: 992288

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus pakistanensis DSM 24834GCA_016908495contigncbi992288
66792Bacillus pakistanensis strain DSM 24834992288.3wgspatric992288
66792Bacillus pakistanensis DSM 248342901141119draftimg992288

GC content

  • @ref: 21524
  • GC-content: 39.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes91.928no
flagellatedyes75.398yes
gram-positiveyes91.477yes
anaerobicno98.905yes
halophileyes58.769no
spore-formingyes96.079no
glucose-utilyes89.657no
aerobicyes93.086yes
thermophileno95.245yes
glucose-fermentno92.923no

External links

@ref: 21524

culture collection no.: DSM 24834, JCM 18975, KCTC 13786, NCCP 168

straininfo link

  • @ref: 88743
  • straininfo: 401765

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24777297Bacillus pakistanensis sp. nov., a halotolerant bacterium isolated from salt mines of the Karak Area in Pakistan.Roohi A, Ahmed I, Paek J, Sin Y, Abbas S, Jamil M, Chang YHAntonie Van Leeuwenhoek10.1007/s10482-014-0177-52014Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Drug Tolerance, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Pakistan, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Salts/*toxicity, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, TemperatureGenetics
Phylogeny34057547Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment.Sun YY, Zhou HZ, Sun QLArch Microbiol10.1007/s00203-021-02401-82021*Bacillus/classification/genetics, Fatty Acids/analysis, *Geologic Sediments/microbiology, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21524Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-24834Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24834)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88743Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401765.1