Strain identifier

BacDive ID: 130153

Type strain: Yes

Species: Microbulbifer maritimus

Strain Designation: TF-17

Strain history: CIP <- 2004, JCM <- 2004, J. H. Yoon, Korea: strain TF-17

NCBI tax ID(s): 247022 (species)

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General

@ref: 21670

BacDive-ID: 130153

DSM-Number: 28870

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Microbulbifer maritimus TF-17 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from intertidal sediment.

NCBI tax id

  • NCBI tax id: 247022
  • Matching level: species

strain history

@refhistory
21670<- CIP; CIP 108504 <- JCM; JCM 12187 <- J. H. Yoon, KRIBB; TF-17
67770J.-H. Yoon TF-17.
120837CIP <- 2004, JCM <- 2004, J. H. Yoon, Korea: strain TF-17

doi: 10.13145/bacdive130153.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Cellvibrionales
  • family: Microbulbiferaceae
  • genus: Microbulbifer
  • species: Microbulbifer maritimus
  • full scientific name: Microbulbifer maritimus Yoon et al. 2004

@ref: 21670

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Microbulbiferaceae

genus: Microbulbifer

species: Microbulbifer maritimus

full scientific name: Microbulbifer maritimus Yoon et al. 2004

strain designation: TF-17

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30138negative4.5 µm0.4 µmrod-shapedno
120837negativerod-shapedyes

pigmentation

  • @ref: 30138
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21670BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40010Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120837CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
21670positivegrowth37mesophilic
30138positivegrowth15-48
30138positiveoptimum37mesophilic
40010positivegrowth37mesophilic
67770positivegrowth37mesophilic
120837positivegrowth25-41
120837nogrowth5psychrophilic
120837nogrowth10psychrophilic

culture pH

@refabilitytypepH
30138positivegrowth7
30138positiveoptimum5.0-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30138aerobe
120837obligate aerobe

spore formation

  • @ref: 30138
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
30138NaClpositivegrowth2.0-10 %
30138NaClpositiveoptimum3 %
120837NaClpositivegrowth2-10 %
120837NaClnogrowth0 %

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3013830089acetate+carbon source
3013829016arginine+carbon source
3013817057cellobiose+carbon source
3013817234glucose+carbon source
3013825115malate+carbon source
3013815361pyruvate+carbon source
3013817822serine+carbon source
3013830031succinate+carbon source
3013827082trehalose+carbon source
3013853426tween 80+carbon source
1208374853esculin-hydrolysis
12083717632nitrate+reduction
12083716301nitrite-reduction
12083717632nitrate-respiration

metabolite production

  • @ref: 120837
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
30138alkaline phosphatase+3.1.3.1
30138gelatinase+
30138cytochrome oxidase+1.9.3.1
120837oxidase+
120837beta-galactosidase-3.2.1.23
120837alcohol dehydrogenase-1.1.1.1
120837gelatinase+
120837amylase+
120837DNase+
120837caseinase-3.4.21.50
120837catalase+1.11.1.6
120837tween esterase-
120837lecithinase-
120837lipase-
120837lysine decarboxylase-4.1.1.18
120837ornithine decarboxylase-4.1.1.17
120837protease+
120837tryptophan deaminase-
120837urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120837-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120837+--+-----++----++-+-----------------------------++---------+-------------------++-------+-+++++-+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21670intertidal sedimentYellow SeaRepublic of KoreaKORAsia
67770Intertidal sediment from the Yellow SeaRepublic of KoreaKORAsia
120837Environment, Intertidal sedimentYellow seaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
216701Risk group (German classification)
1208371Risk group (French classification)

Sequence information

16S sequences

  • @ref: 21670
  • description: Microbulbifer maritimus strain TF-17 16S ribosomal RNA gene, partial sequence
  • accession: AY377986
  • length: 1490
  • database: ena
  • NCBI tax ID: 247022

GC content

@refGC-contentmethod
3013859.9
6777059.9high performance liquid chromatography (HPLC)

External links

@ref: 21670

culture collection no.: DSM 28870, CIP 108504, JCM 12187, KCCM 41774, BCRC 80067

straininfo link

  • @ref: 88706
  • straininfo: 132787

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15280278Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea.Yoon JH, Kim IG, Oh TK, Park YHInt J Syst Evol Microbiol10.1099/ijs.0.02985-02004Alteromonadaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Geologic Sediments/*microbiology, Korea, Molecular Sequence Data, Movement, Pacific Ocean, Phenazines, Phylogeny, Pigments, Biological/biosynthesis, Quinones/analysis/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Spores, Bacterial, Staining and Labeling, Temperature, Water MicrobiologyEnzymology
Phylogeny18768600Microbulbifer halophilus sp. nov., a moderately halophilic bacterium from north-west China.Tang SK, Wang Y, Cai M, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.65519-02008Alteromonadaceae/*classification/genetics/*isolation & purification/physiology, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Flagella, Genes, rRNA, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, *Soil Microbiology, TemperatureGenetics
Phylogeny19244438Microbulbifer donghaiensis sp. nov., isolated from marine sediment of the East China Sea.Wang CS, Wang Y, Xu XW, Zhang DS, Wu YH, Wu MInt J Syst Evol Microbiol10.1099/ijs.0.004531-02009Alteromonadaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny21478393Microbulbifer marinus sp. nov. and Microbulbifer yueqingensis sp. nov., isolated from marine sediment.Zhang DS, Huo YY, Xu XW, Wu YH, Wang CS, Xu XF, Wu MInt J Syst Evol Microbiol10.1099/ijs.0.027714-02011Alteromonadaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics
Phylogeny22155758Microbulbifer taiwanensis sp. nov., isolated from coastal soil.Kampfer P, Arun AB, Young CC, Rekha PD, Martin K, Busse HJ, Chen WMInt J Syst Evol Microbiol10.1099/ijs.0.034512-02011Alteromonadaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Islands, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil/analysis, *Soil Microbiology, TaiwanGenetics
Phylogeny26869238Microbulbifer rhizosphaerae sp. nov., isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum.Camacho M, Del Carmen Montero-Calasanz M, Redondo-Gomez S, Rodriguez-Llorente I, Schumann P, Klenk HPInt J Syst Evol Microbiol10.1099/ijsem.0.0009552016Alteromonadaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Chenopodiaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Spain, Ubiquinone/chemistryGenetics
Phylogeny28905698Microbulbifer aggregans sp. nov., isolated from estuarine sediment from a mangrove forest.Moh TH, Furusawa G, Amirul AAInt J Syst Evol Microbiol10.1099/ijsem.0.0022582017Alteromonadaceae/*classification/genetics/isolation & purification, Avicennia, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Malaysia, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *WetlandsTranscriptome

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21670Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28870Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28870)
30138Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172648928776041
40010Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6134
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
88706Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID132787.1
120837Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108504Collection of Institut Pasteur (CIP 108504)