Strain identifier

BacDive ID: 130140

Type strain: Yes

Species: Actinotignum sanguinis

Strain Designation: IMMIBL-2199

Strain history: <- A. F. Yassin, Inst. Med. Microbiology, Univ. Bonn, Germany; IMMIBL-2199

NCBI tax ID(s): 1445614 (species)

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General

@ref: 22143

BacDive-ID: 130140

DSM-Number: 26039

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Actinotignum sanguinis IMMIBL-2199 is an anaerobe, mesophilic bacterium that was isolated from blood of a human patient with septicaemia.

NCBI tax id

  • NCBI tax id: 1445614
  • Matching level: species

strain history

  • @ref: 22143
  • history: <- A. F. Yassin, Inst. Med. Microbiology, Univ. Bonn, Germany; IMMIBL-2199

doi: 10.13145/bacdive130140.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinotignum
  • species: Actinotignum sanguinis
  • full scientific name: Actinotignum sanguinis Yassin et al. 2015

@ref: 22143

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinotignum

species: Actinotignum sanguinis

full scientific name: Actinotignum sanguinis Yassin et al. 2015

strain designation: IMMIBL-2199

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.994
69480100positive

colony morphology

  • @ref: 63072
  • incubation period: 3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22143PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
22143COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
63072positivegrowth37mesophilic
22143positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
22143anaerobe
63072anaerobe
69480anaerobe95.478

spore formation

@refspore formationconfidence
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380glutamyl-glutamate arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYG
22143--+/-+/---+-----+++/--+-+-

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22143-----+-----+/------++-+/-+-+-+/-+/--+
22143-----+-----------++--+-+-+/-+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
22143blood of a human patient with septicaemiaIngolstadtGermanyDEUEurope48.766511.4258
63072Human bloodIngollstadtGermanyDEUEurope2002-11-01

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_71898.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_188;96_637;97_740;98_859;99_71898&stattab=map
  • Last taxonomy: Actinotignum sanguinis
  • 16S sequence: HG798952
  • Sequence Identity:
  • Total samples: 1313
  • soil counts: 19
  • aquatic counts: 16
  • animal counts: 1278

Safety information

risk assessment

  • @ref: 22143
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22143
  • description: Actinotignum sanguinis partial 16S rRNA gene, type strain IMMIB L-2199T
  • accession: HG798952
  • length: 1508
  • database: ena
  • NCBI tax ID: 1445614

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinotignum sanguinis DSM 26039GCA_003957255contigncbi1445614
66792Actinotignum sanguinis strain DSM 260391445614.3wgspatric1445614
66792Actinotignum sanguinis DSM 260392866239221draftimg1445614

GC content

  • @ref: 22143
  • GC-content: 61.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.599no
gram-positiveyes92.344no
anaerobicyes79.046yes
halophileno70.84no
spore-formingno96.919no
glucose-utilyes89.156no
thermophileno93.911yes
aerobicno93.219yes
flagellatedno97.33no
glucose-fermentyes74.38no

External links

@ref: 22143

culture collection no.: DSM 26039, CCUG 64068

straininfo link

  • @ref: 88694
  • straininfo: 405978

literature

  • topic: Phylogeny
  • Pubmed-ID: 25406238
  • title: Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this species.
  • authors: Yassin AF, Sproer C, Pukall R, Sylvester M, Siering C, Schumann P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.069294-0
  • year: 2014
  • mesh: Actinomycetaceae/*classification/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22143Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26039Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26039)
63072Curators of the CCUGhttps://www.ccug.se/strain?id=64068Culture Collection University of Gothenburg (CCUG) (CCUG 64068)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88694Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405978.1