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Strain identifier

BacDive ID: 13013

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: MD 41.1

Strain history: <- ICPB <- C.B. van Niel; MD 41.1

NCBI tax ID(s): 203192 (species), 316 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 12678

BacDive-ID: 13013

DSM-Number: 50227

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen

description: Pseudomonas stutzeri MD 41.1 is a mesophilic human pathogen of the family Pseudomonadaceae.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history: <- ICPB <- C.B. van Niel; MD 41.1

doi: 10.13145/bacdive13013.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 12678

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: MD 41.1

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes94.825
6948099.675negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12678NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
34811MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
12678positivegrowth28mesophilic
34811positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.262

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 91.927

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44247C10:00.510
    44247C12:019.612
    44247C14:02.114
    44247C16:011.916
    44247C10:0 3OH6.611.423
    44247C12:0 3OH4.213.455
    44247C16:1 ω7c39.915.819
    44247C17:0 CYCLO0.716.888
    44247C18:1 ω7c /12t/9t14.117.824
    44247unknown 11.7980.611.798
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
44247+-------+----+++-++-+
12678+-------+--+-+++-++-+
12678+-------+--+-+++-++-+

Safety information

risk assessment

  • @ref: 12678
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas stutzeri strain DSM 50227 16S rRNA geneU264151456ena316
20218Pseudomonas stutzeri 16S rRNA gene, partial sequenceU586591334ena316

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas stutzeri NCTC10475GCA_900638035completencbi316
66792Pseudomonas stutzeri DSM 50227GCA_002843895scaffoldncbi316
66792Pseudomonas stutzeri strain DSM 50227316.357wgspatric316
66792Pseudomonas stutzeri strain FDAARGOS_1065 strain Not applicable316.579completepatric316
66792Pseudomonas stutzeri strain NCTC10475316.450completepatric316
66792Stutzerimonas kunmingensis DSM 50227GCA_024397485contigncbi1211807
66792Mycobacterium tuberculosis UT0101 UT0101GCA_000656595scaffoldpatric1438892
66792Streptococcus pneumoniae 20893-14GCA_001720085contigpatric1313
66792Escherichia coli SCPM-O-B-8790GCA_011881535contigpatric562
66792Klebsiella pneumoniae IR5712GCA_017311685scaffoldpatric573

External links

@ref: 12678

culture collection no.: CCUG 711, NRRL B-927, NCTC 10475, LMG 1228, CIP 59.54, DSM 50227, ATCC 11607, ICPB 2445, NCIB 9721

straininfo link

@refpassport
20218http://www.straininfo.net/strains/18605
20218http://www.straininfo.net/strains/18599
20218http://www.straininfo.net/strains/18607
20218http://www.straininfo.net/strains/18610

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3115219Immunochemical patterns of distribution of nitrous oxide reductase and nitrite reductase (cytochrome cd1) among denitrifying pseudomonads.Korner H, Frunzke K, Dohler K, Zumft WGArch Microbiol10.1007/BF004296411987Complement Fixation Tests, Cross Reactions, Cytochrome c Group, Cytochromes/*analysis, Immunoassay, Immunodiffusion, Molecular Weight, *Nitrite Reductases, Oxidoreductases/*analysis, Pseudomonas/*enzymology
Metabolism6329754NMR and electron-paramagnetic-resonance studies of a dihaem cytochrome from Pseudomonas stutzeri (ATCC 11607) (cytochrome c peroxidase).Villalain J, Moura I, Liu MC, Payne WJ, LeGall J, Xavier AV, Moura JJEur J Biochem10.1111/j.1432-1033.1984.tb08192.x1984Chemical Phenomena, Chemistry, Cyanides, Cytochrome-c Peroxidase/*metabolism, Electron Spin Resonance Spectroscopy, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Oxidation-Reduction, Peroxidases/*metabolism, Pseudomonas/*enzymology, TemperatureEnzymology
Enzymology9251204Separation of bacterial cells by isoelectric focusing, a new method for analysis of complex microbial communities.Jaspers E, Overmann JAppl Environ Microbiol10.1128/aem.63.8.3176-3181.19971997Bacteria/growth & development/*isolation & purification, Chlorobi/growth & development/isolation & purification, Electrophoresis, Polyacrylamide Gel/methods, Isoelectric Focusing/*methods, Micrococcus luteus/growth & development/isolation & purification, Polymerase Chain Reaction/methods, Pseudomonas/growth & development/isolation & purification, RNA, Ribosomal, 16S/genetics, Sensitivity and Specificity, Water MicrobiologyPhylogeny
Enzymology12459896Ca2+ and the bacterial peroxidases: the cytochrome c peroxidase from Pseudomonas stutzeri.Timoteo CG, Tavares P, Goodhew CF, Duarte LC, Jumel K, Girio FM, Harding S, Pettigrew GW, Moura IJ Biol Inorg Chem10.1007/s00775-002-0382-y2002Animals, Ascorbic Acid/chemistry, *Bacterial Proteins, Binding Sites, Calcium/chemistry/*metabolism/pharmacology, Cytochrome c Group/metabolism, Cytochrome-c Peroxidase/chemistry/isolation & purification/*metabolism, Electron Spin Resonance Spectroscopy, Enzyme Activation, Horses, Hydrogen Peroxide/chemistry, Kinetics, Osmolar Concentration, Oxidation-Reduction, Pseudomonas/cytology/*enzymology/growth & development, Spectrophotometry, UltravioletMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12678Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50227)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50227
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34811Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10027
44247Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 711)https://www.ccug.se/strain?id=711
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)