Strain identifier

BacDive ID: 13007

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: MT-1

Strain history: <- J. Sikorski; MT-1 <- H. Tamegai

NCBI tax ID(s): 203192 (species), 316 (species)

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General

@ref: 6747

BacDive-ID: 13007

DSM-Number: 17085

keywords: Bacteria, aerobe, mesophilic, human pathogen

description: Pseudomonas stutzeri MT-1 is an aerobe, mesophilic human pathogen that was isolated from marine mud.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history

  • @ref: 6747
  • history: <- J. Sikorski; MT-1 <- H. Tamegai

doi: 10.13145/bacdive13007.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 6747

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: MT-1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6747REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)yeshttps://mediadive.dsmz.de/medium/535bName: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
6747REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6747positivegrowth30mesophilic
58904positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 58904
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    58904C10:00.310
    58904C12:010.212
    58904C14:00.714
    58904C15:00.315
    58904C16:01616
    58904C18:00.418
    58904C10:0 3OH411.423
    58904C12:0 3OH313.455
    58904C13:0 ISO 2OH0.813.814
    58904C16:1 ω7c3115.819
    58904C17:0 CYCLO0.316.888
    58904C17:0 iso0.316.629
    58904C18:1 ω7c /12t/9t29.817.824
    58904C18:1 ω9c0.217.769
    58904C18:2 ω6,9c/C18:0 ANTE0.317.724
    58904C19:0 CYCLO ω8c1.818.9
    58904Unidentified0.415.272
    58904unknown 11.7980.211.798
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
58904-+++-++-+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
58904+-------++-+-++++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountrysampling date
6747marine mudMariana Trench, 10898 m depthInternational Waters
58904Marine sedimentMariana Trench,10898 m depthInternational Waters1996-02-01

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#Sediment

Safety information

risk assessment

  • @ref: 6747
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 6747

culture collection no.: DSM 17085, CCUG 50545

straininfo link

  • @ref: 82217
  • straininfo: 214540

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology17690469Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1.Tamegai H, Ikeda E, Kato C, Horikoshi KBiosci Biotechnol Biochem10.1271/bbb.702562007*Multigene Family, Nitrate Reductase/*genetics, Nitrites/*metabolism, Open Reading Frames, Oxidation-Reduction, Periplasmic Proteins/genetics, Pseudomonas/*enzymology, Seawater/*microbiology, Sequence Homology, Nucleic AcidMetabolism
Enzymology19352028Physiological roles of two dissimilatory nitrate reductases in the deep-sea denitrifier Pseudomonas sp. strain MT-1.Ikeda E, Andou S, Iwama U, Kato C, Horikoshi K, Tamegai HBiosci Biotechnol Biochem10.1271/bbb.808332009Cell Membrane/*metabolism, Gene Expression, Nitrate Reductase/biosynthesis/chemistry/genetics/*metabolism, Nitrogen/*metabolism, Oceans and Seas, Periplasm/*enzymology, Pseudomonas/*enzymology, Pseudomonas stutzeri/enzymology, Reverse Transcriptase Polymerase Chain Reaction, SolubilityMetabolism

Reference

@idauthorscataloguedoi/urltitle
6747Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17085)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17085
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
58904Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50545)https://www.ccug.se/strain?id=50545
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
82217Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID214540.1StrainInfo: A central database for resolving microbial strain identifiers