Strain identifier
BacDive ID: 13006
Type strain:
Species: Pseudomonas stutzeri
Strain Designation: 24a75
Strain history: <- J. Sikorski
NCBI tax ID(s): 203192 (species), 316 (species)
General
@ref: 6746
BacDive-ID: 13006
DSM-Number: 17084
keywords: genome sequence, Bacteria, aerobe, mesophilic, motile, human pathogen
description: Pseudomonas stutzeri 24a75 is an aerobe, mesophilic, motile human pathogen that was isolated from soil contaminated with mineral oil.
NCBI tax id
NCBI tax id | Matching level |
---|---|
203192 | species |
316 | species |
strain history
- @ref: 6746
- history: <- J. Sikorski
doi: 10.13145/bacdive13006.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas stutzeri
- full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)
synonyms
@ref synonym 20215 Stutzerimonas chloritidismutans 20215 Pseudomonas perfectomarinus 20215 Bacterium stutzeri 20215 Pseudomonas kunmingensis 20215 Pseudomonas chloritidismutans 20215 Stutzerimonas kunmingensis 20215 Stutzerimonas stutzeri 20215 Stutzerimonas perfectomarina 20215 Pseudomonas perfectomarina
@ref: 6746
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas stutzeri
full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946
strain designation: 24a75
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | yes | 98.148 | |
69480 | 99.999 | negative |
Culture and growth conditions
culture medium
- @ref: 6746
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6746 | positive | growth | 30 | mesophilic |
58899 | positive | growth | 30-37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 58899
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.983 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 58899 C12:0 8.6 12 58899 C14:0 1.6 14 58899 C16:0 28.2 16 58899 C18:0 1.5 18 58899 C10:0 3OH 2.8 11.423 58899 C12:0 3OH 2.3 13.455 58899 C16:1 ω7c 11.1 15.819 58899 C17:0 CYCLO 13.6 16.888 58899 C18:1 ω7c /12t/9t 16.1 17.824 58899 C18:2 ω6,9c/C18:0 ANTE 1.8 17.724 58899 C19:0 CYCLO ω8c 12.4 18.9 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
58899 | - | - | + | + | - | + | + | - | + | - | - | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
58899 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
6746 | soil contaminated with mineral oil | Espelkamp | Germany | DEU | Europe | |
58899 | Soil contaminated with mineral oil | Espelkamp | Germany | DEU | Europe | 2000-01-01 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Contamination | #Oil (Fuel) |
#Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
- @ref: 6746
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Stutzerimonas stutzeri 24a75 | GCA_002890915 | contig | ncbi | 316 |
66792 | Pseudomonas stutzeri strain 24a75 | 316.369 | wgs | patric | 316 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 98.716 | no |
anaerobic | no | 97.827 | yes |
halophile | no | 86.765 | no |
spore-forming | no | 94.695 | no |
glucose-util | yes | 83.109 | yes |
motile | yes | 91.649 | no |
flagellated | yes | 90.122 | no |
aerobic | yes | 88.882 | yes |
thermophile | no | 99.745 | yes |
glucose-ferment | no | 91.716 | yes |
External links
@ref: 6746
culture collection no.: DSM 17084, CCUG 50540
straininfo link
- @ref: 82216
- straininfo: 214535
literature
- topic: Phylogeny
- Pubmed-ID: 16166664
- title: Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment.
- authors: Sikorski J, Lalucat J, Wackernagel W
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63535-0
- year: 2005
- mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal Spacer/analysis, Genes, rRNA, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Pseudomonas stutzeri/*classification/*genetics/isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Seawater/*microbiology, *Soil Microbiology
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
6746 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17084) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17084 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
58899 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 50540) | https://www.ccug.se/strain?id=50540 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
82216 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID214535.1 | StrainInfo: A central database for resolving microbial strain identifiers |