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Strain identifier

BacDive ID: 13004

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: 4C29

Strain history: <- J. Sikorski; 4C29

NCBI tax ID(s): 203192 (species), 316 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6744

BacDive-ID: 13004

DSM-Number: 17082

keywords: genome sequence, Bacteria, aerobe, mesophilic, human pathogen

description: Pseudomonas stutzeri 4C29 is an aerobe, mesophilic human pathogen that was isolated from wadden sea sediment.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history: <- J. Sikorski; 4C29

doi: 10.13145/bacdive13004.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 6744

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: 4C29

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes90.685
6948099.519negative

Culture and growth conditions

culture medium

  • @ref: 6744
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6744positivegrowth30mesophilic
58897positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 58897
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 97.899

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 79.514

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    58897C10:00.210
    58897C12:08.512
    58897C14:01.414
    58897C15:00.415
    58897C16:018.516
    58897C10:0 3OH3.911.423
    58897C11:0 ISO 3OH0.212.09
    58897C12:0 3OH2.313.455
    58897C13:0 iso0.112.612
    58897C13:0 ISO 2OH0.813.814
    58897C16:1 ω7c3515.819
    58897C17:0 iso0.416.629
    58897C17:1 ω8c0.216.792
    58897C18:1 ω7c /12t/9t2717.824
    58897C18:1 ω9c0.217.769
    58897C18:2 ω6,9c/C18:0 ANTE0.217.724
    58897C19:0 CYCLO ω8c0.318.9
    58897Unidentified0.515.269
    58897unknown 11.7980.111.798
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
58897-+++-+--+-----------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
58897+-------+--+--+-++--+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
6744wadden sea sedimentDangast, North Sea coastGermanyDEUEurope
58897Wadden sea sedimentDangast,North Sea coastGermanyDEUEurope1999-09-01

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat

Safety information

risk assessment

  • @ref: 6744
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas stutzeri 4C29GCA_002890895contigncbi316
66792Pseudomonas stutzeri strain 4C29316.368wgspatric316
66792Streptococcus pneumoniae SMRU1647GCA_001118565scaffoldpatric1313

External links

@ref: 6744

culture collection no.: DSM 17082, CCUG 50538

straininfo link

@refpassport
20218http://www.straininfo.net/strains/691198
20218http://www.straininfo.net/strains/531753

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6744Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17082)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17082
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
58897Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50538)https://www.ccug.se/strain?id=50538
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)