Strain identifier

BacDive ID: 13002

Type strain: No

Species: Pseudomonas stutzeri

Strain Designation: 320, 62-HA

Strain history: CIP <- 2002, NCIMB

NCBI tax ID(s): 203192 (species), 316 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5022

BacDive-ID: 13002

DSM-Number: 13627

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas stutzeri 320 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from clinical specimen.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history

@refhistory
5022<- T. Wunder, THOR GmbH, Speyer, Germany <- NCIMB <- ATCC
338592002, NCIMB
123336CIP <- 2002, NCIMB

doi: 10.13145/bacdive13002.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 5022

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: 320, 62-HA

type strain: no

Morphology

cell morphology

  • @ref: 123336
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 123336
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5022REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33859MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123336CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5022positivegrowth30mesophilic
33859positivegrowth30mesophilic
123336positivegrowth25-41
123336nogrowth5psychrophilic
123336nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123336
  • oxygen tolerance: obligate aerobe

halophily

  • @ref: 123336
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12333616947citrate+carbon source
1233364853esculin-hydrolysis
12333617632nitrate+reduction
12333616301nitrite+reduction
12333617632nitrate-respiration

antibiotic resistance

  • @ref: 123336
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12333635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
123336oxidase+
123336beta-galactosidase-3.2.1.23
123336alcohol dehydrogenase-1.1.1.1
123336gelatinase-
123336amylase+
123336DNase-
123336caseinase-3.4.21.50
123336catalase+1.11.1.6
123336tween esterase-
123336lecithinase-
123336lipase-
123336lysine decarboxylase-4.1.1.18
123336ornithine decarboxylase-4.1.1.17
123336protease-
123336tryptophan deaminase-
123336urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123336-+++-+--+--+--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5022+-------+----+-+-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123336++-------++--------------------+-----------++----++--+------+--------------+++-++++-+--+++++++-++++

Isolation, sampling and environmental information

isolation

@refsample type
5022clinical specimen
123336Clinical specimen

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Specimen
#Infection#Medical environment#Clinic

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5022yes, in single cases1Risk group (German classification)
1233361Risk group (French classification)

External links

@ref: 5022

culture collection no.: DSM 13627, ATCC 17686, CIP 107719, NCIMB 11359, NRRL B-3389

straininfo link

  • @ref: 82212
  • straininfo: 36711

Reference

@idauthorscataloguedoi/urltitle
5022Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13627)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13627
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33859Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5186
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
82212Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36711.1StrainInfo: A central database for resolving microbial strain identifiers
123336Curators of the CIPCollection of Institut Pasteur (CIP 107719)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107719