Strain identifier
BacDive ID: 13
Type strain:
Species: Acetobacter malorum
Strain history: CIP <- 2005, DSMZ <- LMG <- J. Swings, Ghent Univ., Ghent, Belgium
NCBI tax ID(s): 1307910 (strain), 178901 (species)
General
@ref: 5310
BacDive-ID: 13
DSM-Number: 14337
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, ellipsoidal, colony-forming
description: Acetobacter malorum DSM 14337 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from rotting apple.
NCBI tax id
NCBI tax id | Matching level |
---|---|
178901 | species |
1307910 | strain |
strain history
@ref | history |
---|---|
5310 | <- I. Cleenwerck, LMG <- J. Swings, Univ. Ghent, Belgium |
67770 | LMG 1746 <-- J. Swings 76.10. |
120763 | CIP <- 2005, DSMZ <- LMG <- J. Swings, Ghent Univ., Ghent, Belgium |
doi: 10.13145/bacdive13.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Acetobacter
- species: Acetobacter malorum
- full scientific name: Acetobacter malorum Cleenwerck et al. 2002
@ref: 5310
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Acetobacter
species: Acetobacter malorum
full scientific name: Acetobacter malorum Cleenwerck et al. 2002
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
23233 | negative | 1.1-1.3 µm | 0.9 µm | ellipsoidal | no |
120763 | negative | rod-shaped | no |
colony morphology
- @ref: 23233
- colony size: 0.5 mm
- colony color: beige
- colony shape: circular
- medium used: YPM agar
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5310 | YPM MEDIUM (DSMZ Medium 360) | yes | https://mediadive.dsmz.de/medium/360 | Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water |
23233 | YPM agar | yes | ||
37644 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
120763 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
120763 | CIP Medium 314 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5310 | positive | growth | 28 | mesophilic |
37644 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
120763 | positive | growth | 22-30 | |
120763 | no | growth | 10 | psychrophilic |
120763 | no | growth | 37 | mesophilic |
120763 | no | growth | 41 | thermophilic |
120763 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23233 | obligate aerobe |
120763 | obligate aerobe |
spore formation
- @ref: 23233
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120763 | NaCl | positive | growth | 0 % |
120763 | NaCl | no | growth | 2 % |
120763 | NaCl | no | growth | 4 % |
120763 | NaCl | no | growth | 6 % |
120763 | NaCl | no | growth | 8 % |
120763 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23233 | 16236 | ethanol | - | carbon source |
23233 | 17306 | maltose | - | carbon source |
23233 | 28938 | ammonium | - | nitrogen source |
23233 | 17790 | methanol | + | carbon source |
120763 | 606565 | hippurate | + | hydrolysis |
120763 | 17632 | nitrate | + | reduction |
120763 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23233 | 17426 | 5-dehydro-D-gluconate | no |
23233 | 16808 | 2-dehydro-D-gluconate | yes |
120763 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23233 | catalase | + | 1.11.1.6 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
120763 | oxidase | - | |
120763 | beta-galactosidase | - | 3.2.1.23 |
120763 | gelatinase | - | |
120763 | catalase | + | 1.11.1.6 |
120763 | gamma-glutamyltransferase | - | 2.3.2.2 |
120763 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120763 | - | - | + | + | - | + | + | - | - | - | - | + | - | - | - | - | - | + | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120763 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
5310 | rotting apple | Gent | Belgium | BEL | Europe |
67770 | Rotting apple | Ghent | Belgium | BEL | Europe |
120763 | Food, Rotting apple | Belgium | BEL | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Decomposing plant |
#Host Body-Site | #Plant | #Bark |
#Host Body-Site | #Plant | #Fruit (Seed) |
taxonmaps
- @ref: 69479
- File name: preview.99_1214.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_961;99_1214&stattab=map
- Last taxonomy: Acetobacter
- 16S sequence: JF793957
- Sequence Identity:
- Total samples: 1514
- soil counts: 185
- aquatic counts: 172
- animal counts: 932
- plant counts: 225
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5310 | 1 | Risk group (German classification) |
120763 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Acetobacter malorum strain DSM 14337 16S ribosomal RNA gene, partial sequence | JF793957 | 1353 | ena | 178901 |
20218 | Acetobacter malorum gene for 16S rRNA, partial sequence, strain: JCM 17274 | AB665081 | 1412 | ena | 178901 |
20218 | Acetobacter malorum 16S rRNA gene, strain LMG 1746 | AJ419844 | 1442 | ena | 178901 |
20218 | Acetobacter malorum strain LMG 1746 16S ribosomal RNA gene, partial sequence | JF793956 | 1355 | ena | 178901 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Acetobacter malorum strain LMG 1746 | 178901.12 | wgs | patric | 178901 |
66792 | Acetobacter malorum LMG 1746 | 2713897233 | draft | img | 178901 |
67770 | Acetobacter malorum LMG 1746 | GCA_001580615 | contig | ncbi | 178901 |
GC content
@ref | GC-content | method |
---|---|---|
23233 | 57.20 | high performance liquid chromatography (HPLC) |
67770 | 57.2 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 69.22 | yes |
gram-positive | no | 97.052 | yes |
anaerobic | no | 98.806 | yes |
aerobic | yes | 88.688 | yes |
halophile | no | 94.066 | no |
spore-forming | no | 94.818 | yes |
thermophile | no | 98.377 | no |
glucose-util | yes | 81.661 | no |
flagellated | no | 83.817 | yes |
glucose-ferment | no | 89.465 | no |
External links
@ref: 5310
culture collection no.: DSM 14337, CIP 108768, LMG 1746, JCM 17274, ATCC 14337, BCRC 17641, VTT E-042462
straininfo link
- @ref: 69697
- straininfo: 5
literature
- topic: Phylogeny
- Pubmed-ID: 12361257
- title: Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.
- authors: Cleenwerck I, Vandemeulebroecke K, Janssens D, Swings J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-52-5-1551
- year: 2002
- mesh: Acetobacter/*classification/*genetics/isolation & purification/metabolism, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Terminology as Topic
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5310 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14337) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14337 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23233 | I. Cleenwerck, K. Vandemeulebroecke, D. Janssens and J. Swings | 10.1099/ijs.0.02064-0 | Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. | IJSEM 52: 1551-1558 2002 | ||
37644 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6433 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69697 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID5.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120763 | Curators of the CIP | Collection of Institut Pasteur (CIP 108768) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108768 |