Strain identifier
BacDive ID: 12999
Type strain:
Species: Pseudomonas stutzeri
Strain Designation: KC
Strain history: CIP <- 2002, L. Sepulveda, Univ. San Juan, Puerto Rico: strain KC
NCBI tax ID(s): 203192 (species), 316 (species)
General
@ref: 2900
BacDive-ID: 12999
DSM-Number: 7136
keywords: genome sequence, Bacteria, aerobe, mesophilic, motile, human pathogen
description: Pseudomonas stutzeri KC is an aerobe, mesophilic, motile human pathogen that was isolated from aquifer cores.
NCBI tax id
NCBI tax id | Matching level |
---|---|
203192 | species |
316 | species |
strain history
@ref | history |
---|---|
2900 | <- C.S. Criddle (Pseudomonas sp. KC) |
36141 | 2002, L. Sepulveda, Univ. San Juan, Puerto Rico: strain KC |
123332 | CIP <- 2002, L. Sepulveda, Univ. San Juan, Puerto Rico: strain KC |
doi: 10.13145/bacdive12999.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas stutzeri
- full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)
synonyms
@ref synonym 20215 Stutzerimonas chloritidismutans 20215 Pseudomonas perfectomarinus 20215 Bacterium stutzeri 20215 Pseudomonas kunmingensis 20215 Pseudomonas chloritidismutans 20215 Stutzerimonas kunmingensis 20215 Stutzerimonas stutzeri 20215 Stutzerimonas perfectomarina 20215 Pseudomonas perfectomarina
@ref: 2900
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas stutzeri
full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946
strain designation: KC
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 98.998 | ||
69480 | 99.997 | negative | ||
123332 | yes | negative | rod-shaped |
pigmentation
- @ref: 123332
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2900 | ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE (DSMZ Medium 471) | yes | https://mediadive.dsmz.de/medium/471 | Name: ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE (DSMZ Medium 471; with strain-specific modifications) Composition: K2HPO4 5.10978 g/l Sodium acetate 3.0 g/l KNO3 1.99601 g/l KH2PO4 1.49701 g/l Na-benzoate 0.918164 g/l NH4Cl 0.299401 g/l MgSO4 x 7 H2O 0.199601 g/l CaCl2 0.00998004 g/l EDTA 0.000998004 g/l FeSO4 x 7 H2O 0.000399202 g/l H3BO3 5.98802e-05 g/l CoCl2 x 6 H2O 3.99202e-05 g/l ZnSO4 x 7 H2O 1.99601e-05 g/l MnCl2 x 4 H2O 5.98802e-06 g/l Na2MoO4 x 2 H2O 5.98802e-06 g/l NiCl2 x 6 H2O 3.99202e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water |
2900 | REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) | yes | https://mediadive.dsmz.de/medium/1a | Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
36141 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
123332 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2900 | positive | growth | 30 | mesophilic |
36141 | positive | growth | 30 | mesophilic |
123332 | positive | growth | 5-41 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
2900 | aerobe |
2900 | anaerobe |
123332 | obligate aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.915 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
123332 | NaCl | positive | growth | 0-6 % |
123332 | NaCl | no | growth | 8 % |
123332 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
123332 | 16947 | citrate | + | carbon source |
123332 | 4853 | esculin | - | hydrolysis |
123332 | 17632 | nitrate | + | reduction |
123332 | 16301 | nitrite | + | reduction |
123332 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 123332
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
123332 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
123332 | oxidase | + | |
123332 | beta-galactosidase | - | 3.2.1.23 |
123332 | alcohol dehydrogenase | - | 1.1.1.1 |
123332 | gelatinase | - | |
123332 | amylase | + | |
123332 | DNase | - | |
123332 | caseinase | - | 3.4.21.50 |
123332 | catalase | + | 1.11.1.6 |
123332 | tween esterase | + | |
123332 | lecithinase | + | |
123332 | lipase | - | |
123332 | lysine decarboxylase | - | 4.1.1.18 |
123332 | ornithine decarboxylase | - | 4.1.1.17 |
123332 | protease | + | |
123332 | tryptophan deaminase | - | |
123332 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123332 | - | + | + | + | - | + | - | - | + | - | - | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2900 | + | - | - | - | - | - | - | - | + | - | - | + | - | + | + | + | - | + | + | - | + |
2900 | + | - | - | - | - | - | - | - | + | - | - | + | - | + | + | + | - | + | + | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123332 | + | + | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | + | + | - | - | + | + | + | - | - | - | - | + | - | + | - | - | + | - | - | - | - | - | - | - | + | - | + | + | + | - | + | + | + | + | - | + | - | + | + | + | + | + | + | + | + | - | + | + | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
2900 | aquifer cores | California | USA | USA | North America |
123332 | Aquifer cores |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Groundwater
Safety information
risk assessment
@ref | pathogenicity human | biosafety level | biosafety level comment |
---|---|---|---|
2900 | yes, in single cases | 1 | Risk group (German classification) |
123332 | 1 | Risk group (French classification) |
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudomonas stutzeri strain KC | 316.364 | wgs | patric | 316 |
66792 | Stutzerimonas stutzeri KC | GCA_002890795 | contig | ncbi | 316 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 98.847 | no |
anaerobic | no | 97.718 | no |
halophile | no | 86.569 | no |
spore-forming | no | 95.19 | no |
glucose-util | yes | 93.221 | no |
motile | yes | 91.425 | no |
flagellated | yes | 90.468 | no |
aerobic | yes | 91.898 | no |
thermophile | no | 99.77 | yes |
glucose-ferment | no | 91.799 | no |
External links
@ref: 2900
culture collection no.: DSM 7136, CIP 107462, ATCC 55595
straininfo link
- @ref: 82209
- straininfo: 43628
literature
- topic: Phylogeny
- Pubmed-ID: 11760942
- title: Pseudomonas sp. strain KC represents a new genomovar within Pseudomonas stutzeri.
- authors: Sepulveda-Torres LC, Zhou J, Guasp C, Lalucat J, Knaebel D, Plank JL, Criddle CS
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-51-6-2013
- year: 2001
- mesh: Biodegradation, Environmental, Carbon Tetrachloride/*metabolism, DNA Gyrase/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Inositol/metabolism, Molecular Sequence Data, Nitrites/metabolism, Nucleic Acid Hybridization, Phenotype, *Phylogeny, Polymerase Chain Reaction, Pseudomonas/*classification/*genetics/metabolism, Pyridines/chemistry/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
2900 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7136) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7136 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
36141 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4900 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
82209 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43628.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
123332 | Curators of the CIP | Collection of Institut Pasteur (CIP 107462) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107462 |