Strain identifier

BacDive ID: 12997

Type strain: No

Species: Stutzerimonas frequens

Strain Designation: DNSP21

Strain history: CIP <- 2002, CCUG <- J. Lalucat/R. Rossello-Mora, Palma de Mallorca, Spain: strain DNSP21

NCBI tax ID(s): 203192 (species), 316 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2441

BacDive-ID: 12997

DSM-Number: 6082

keywords: genome sequence, Bacteria, obligate aerobe, mesophilic, motile, human pathogen

description: Stutzerimonas frequens DNSP21 is an obligate aerobe, mesophilic, motile human pathogen that was isolated from sewage water.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history

@refhistory
2441<- R.A. Rosselló; DNSP21
123334CIP <- 2002, CCUG <- J. Lalucat/R. Rossello-Mora, Palma de Mallorca, Spain: strain DNSP21

doi: 10.13145/bacdive12997.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Stutzerimonas
  • species: Stutzerimonas frequens
  • full scientific name: Stutzerimonas frequens Gomila et al. 2022

@ref: 2441

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

strain designation: DNSP21

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.65
69480100negative
123334yesnegativerod-shaped

pigmentation

  • @ref: 123334
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2441REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
39926MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123334CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
2441positivegrowth30mesophilic
39926positivegrowth30mesophilic
123334positivegrowth10-41
123334nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123334
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.989

halophily

@refsaltgrowthtested relationconcentration
123334NaClpositivegrowth0-8 %
123334NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12333416947citrate+carbon source
1233344853esculin-hydrolysis
12333417632nitrate+reduction
12333416301nitrite+reduction
12333417632nitrate-respiration

antibiotic resistance

  • @ref: 123334
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12333435581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
123334oxidase+
123334beta-galactosidase-3.2.1.23
123334alcohol dehydrogenase-1.1.1.1
123334gelatinase-
123334amylase+
123334DNase-
123334caseinase-3.4.21.50
123334catalase+1.11.1.6
123334tween esterase+
123334lecithinase-
123334lipase+
123334lysine decarboxylase-4.1.1.18
123334ornithine decarboxylase-4.1.1.17
123334protease+
123334tryptophan deaminase-
123334urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123334-++++++-+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2441+-------+----+++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123334++-------++---------------+----+-+-----------+---++--+------+------------+-+++-++++----+++++++-++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2441sewage waterMallorca, Santa PoncaSpainESPEurope
123334Environment, Sewage waterSpainESPEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
2441yes, in single cases1Risk group (German classification)
1233341Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Stutzerimonas frequens DNSP21GCA_002890935contigncbi2968969
66792Pseudomonas stutzeri strain DNSP21316.370wgspatric316
66792Pseudomonas stutzeri strain FDAARGOS_877316.577completepatric316

GC content

  • @ref: 2441
  • GC-content: 63.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.978no
anaerobicno97.994no
halophileno78.137no
spore-formingno95.391no
glucose-utilyes90.419yes
motileyes90.899no
flagellatedyes89.278no
aerobicyes91.738no
thermophileno99.682yes
glucose-fermentno90.988yes

External links

@ref: 2441

culture collection no.: DSM 6082, LMG 18520, CIP 107691, CCUG 44594

straininfo link

  • @ref: 82207
  • straininfo: 12138

literature

  • topic: Metabolism
  • Pubmed-ID: 17473886
  • title: Identification of siderophores of Pseudomonas stutzeri.
  • authors: Zawadzka AM, Vandecasteele FP, Crawford RL, Paszczynski AJ
  • journal: Can J Microbiol
  • DOI: 10.1139/w06-077
  • year: 2006
  • mesh: Bacterial Outer Membrane Proteins/genetics/metabolism, Chromatography, High Pressure Liquid, Deferoxamine/chemistry/metabolism, Ferric Compounds/chemistry/metabolism, Gas Chromatography-Mass Spectrometry, Molecular Structure, Oligopeptides/chemistry/metabolism, Pseudomonas stutzeri/classification/genetics/*metabolism, Receptors, Cell Surface/genetics/metabolism, Siderophores/chemistry/*metabolism, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
  • topic2: Proteome

Reference

@idauthorscataloguedoi/urltitle
2441Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6082)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6082
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39926Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5154
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82207Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12138.1StrainInfo: A central database for resolving microbial strain identifiers
123334Curators of the CIPCollection of Institut Pasteur (CIP 107691)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107691