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Strain identifier

BacDive ID: 1298

Type strain: Yes

Species: Salibacterium halochares

Strain history: <- A.-I. Koukkou <- P. Lazoura, Univ. Ioannina, Dept. Chemistry, Greece; MSS4

NCBI tax ID(s): 520043 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15655

BacDive-ID: 1298

DSM-Number: 21373

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Salibacterium halochares DSM 21373 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from solar salterns.

NCBI tax id

  • NCBI tax id: 520043
  • Matching level: species

strain history: <- A.-I. Koukkou <- P. Lazoura, Univ. Ioannina, Dept. Chemistry, Greece; MSS4

doi: 10.13145/bacdive1298.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Salibacterium
  • species: Salibacterium halochares
  • full scientific name: Salibacterium halochares (Pappa et al. 2010) Reddy et al. 2015
  • synonyms

    • @ref: 20215
    • synonym: Bacillus halochares

@ref: 15655

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Salibacterium

species: Salibacterium halochares

full scientific name: Salibacterium halochares (Pappa et al. 2010) Reddy et al. 2015

type strain: yes

Morphology

cell morphology

  • @ref: 29494
  • gram stain: positive
  • cell length: 2.6 µm
  • cell width: 0.6 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 15655
  • name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514d
  • composition: Name: MODIFIED BACTO MARINE BROTH (DSMZ Medium 514d) Composition: Difco marine broth 37.4 g/l Agar 15.0 g/l Malt extract 1.0 g/l Soy peptone 1.0 g/l Pancreatic digest of casein 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15655positivegrowth30mesophilic
29494positivegrowth26-45
29494positiveoptimum37mesophilic

culture pH

@refabilitytypepHPH range
29494positivegrowth06-09alkaliphile
29494positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 29494
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29494
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29494NaClpositivegrowth5.8-23.2 %
29494NaClpositiveoptimum14.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2949430089acetate+carbon source
2949417057cellobiose+carbon source
2949417754glycerol+carbon source
2949417716lactose+carbon source
2949417306maltose+carbon source
2949429864mannitol+carbon source
2949433942ribose+carbon source
2949417992sucrose+carbon source
294944853esculin+hydrolysis

enzymes

@refvalueactivityec
29494catalase+1.11.1.6
29494cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 15655
  • sample type: solar salterns
  • geographic location: Mesolongi
  • country: Greece
  • origin.country: GRC
  • continent: Europe

isolation source categories

  • Cat1: #Condition
  • Cat2: #Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_74380.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_605;96_7566;97_17484;98_52038;99_74380&stattab=map
  • Last taxonomy: Salibacterium halochares subclade
  • 16S sequence: AM982516
  • Sequence Identity:
  • Total samples: 1
  • animal counts: 1

Safety information

risk assessment

  • @ref: 15655
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15655
  • description: Bacillus halochares partial 16S rRNA gene, strain MSS4
  • accession: AM982516
  • length: 1426
  • database: ena
  • NCBI tax ID: 520043

GC content

@refGC-contentmethod
1565547.2thermal denaturation, midpoint method (Tm)
2949447.2

External links

@ref: 15655

culture collection no.: DSM 21373, LMG 24571, MSS 4

straininfo link

@refpassport
20218http://www.straininfo.net/strains/854875
20218http://www.straininfo.net/strains/804617

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19671720Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern.Pappa A, Sanchez-Porro C, Lazoura P, Kallimanis A, Perisynakis A, Ventosa A, Drainas C, Koukkou AIInt J Syst Evol Microbiol10.1099/ijs.0.014233-02009Bacillus/classification/*genetics/growth & development/isolation & purification, Base Composition, DNA, Bacterial/genetics/metabolism, Greece, Hydrogen-Ion Concentration, Molecular Sequence Data, Monosaccharides/metabolism, Nitrates/metabolism, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Spores, Bacterial/physiology, Urease/metabolismMetabolism
Phylogeny26338019Salibacterium halotolerans gen. nov., sp. nov., a bacterium isolated from a salt pan, reclassification of Bacillus qingdaonensis as Salibacterium qingdaonense comb. nov. and Bacillus halochares as Salibacterium halochares comb. nov.Vishnuvardhan Reddy S, Thirumala M, Sasikala C, Venkata Ramana CInt J Syst Evol Microbiol10.1099/ijsem.0.0005722015Bacillaceae/*classification/genetics/isolation & purification, Bacillus/*classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium ChlorideTranscriptome
Phylogeny29148364Salibacterium lacus sp. nov., a halophilic, non-spore-forming bacterium isolated from sediment of a saline lake.Wang YQ, Liu XM, Tang J, Yang GQ, Yu ZInt J Syst Evol Microbiol10.1099/ijsem.0.0024672017Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Lakes/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny30226463Salibacterium nitratireducens sp. nov., a haloalkalitolerant bacterium isolated from a water sample from Sambhar salt lake, India.Singh H, Kaur M, Singh S, Mishra S, Kumar S, Vemuluri VR, Tanuku NRS, Pinnaka AKInt J Syst Evol Microbiol10.1099/ijsem.0.0030212018Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Lakes/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
15655Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21373)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21373
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2589410.1099/ijs.0.014233-019671720
29494Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125894
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta