Strain identifier

BacDive ID: 1293

Type strain: Yes

Species: Salipaludibacillus neizhouensis

Strain history: <- Y.-G. Chen and S.-K. Tang, YIM <- H.-D. Xiao; JSM 071004

NCBI tax ID(s): 885475 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8323

BacDive-ID: 1293

DSM-Number: 19794

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Salipaludibacillus neizhouensis DSM 19794 is an aerobe, spore-forming, mesophilic bacterium that was isolated from homogenates of sea anemone.

NCBI tax id

  • NCBI tax id: 885475
  • Matching level: species

strain history

  • @ref: 8323
  • history: <- Y.-G. Chen and S.-K. Tang, YIM <- H.-D. Xiao; JSM 071004

doi: 10.13145/bacdive1293.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Salipaludibacillus
  • species: Salipaludibacillus neizhouensis
  • full scientific name: Salipaludibacillus neizhouensis (Chen et al. 2009) Sultanpuram and Mothe 2016
  • synonyms

    • @ref: 20215
    • synonym: Bacillus neizhouensis

@ref: 8323

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Salipaludibacillus

species: Salipaludibacillus neizhouensis

full scientific name: Salipaludibacillus neizhouensis (Chen et al. 2009) Sultanpuram and Mothe 2016

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29252positive3.0-5.0 µm0.5-0.6 µmrod-shapedno
43624positiverod-shapedno
69480yes91.467
69480positive100

colony morphology

  • @ref: 43624
  • colony color: Pale yellow

Culture and growth conditions

culture medium

  • @ref: 8323
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l MnSO4 0.01 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
8323positivegrowth30mesophilic
29252positivegrowth04-30
29252positiveoptimum25mesophilic
43624positivegrowth4-30
43624positiveoptimum25mesophilic

culture pH

@refabilitytypepHPH range
29252positivegrowth6.5-10.0alkaliphile
29252positiveoptimum8.5
43624positivegrowth6.5-10
43624positiveoptimum8.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29252aerobe
43624facultative anaerobe

spore formation

@refspore formationspore descriptiontype of sporeconfidence
29252yes
43624yesoval or ellipsoidal spores ath the sub-terminal positionendospore
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
29252NaClpositivegrowth0.5-10 %
29252NaClpositiveoptimum02-04 %
43624NaClpositivegrowth0.5-10 %(w/v)
43624NaClpositiveoptimum2-4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2925228757fructose+carbon source
2925217234glucose+carbon source
2925225017leucine+carbon source
2925217306maltose+carbon source
2925217992sucrose+carbon source
2925227082trehalose+carbon source
292524853esculin+hydrolysis
2925217632nitrate+reduction
4362416947citrate-assimilation
4362427897tryptophan-energy source
4362417234glucose-fermentation
43624casein-hydrolysis
4362462968cellulose-hydrolysis
4362416991dna-hydrolysis
436244853esculin-hydrolysis
436245291gelatin-hydrolysis
4362428017starch-hydrolysis
4362453424tween 20+hydrolysis
4362417632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
4362415688acetoinno
4362416136hydrogen sulfideno
4362435581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
4362417234glucose-
4362415688acetoin-
4362435581indole-

enzymes

@refvalueactivityec
29252alkaline phosphatase+3.1.3.1
29252catalase+1.11.1.6
29252gelatinase+
29252cytochrome oxidase+1.9.3.1
43624catalase+1.11.1.6
43624cytochrome oxidase+1.9.3.1
43624urease-3.5.1.5
43624tryptophan deaminase-4.1.99.1

Isolation, sampling and environmental information

isolation

  • @ref: 8323
  • sample type: homogenates of sea anemone
  • geographic location: South China Sea, Neizhou Bay
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Cnidaria (Corals)

taxonmaps

  • @ref: 69479
  • File name: preview.99_102603.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_144;97_155;98_33125;99_102603&stattab=map
  • Last taxonomy: Salipaludibacillus neizhouensis subclade
  • 16S sequence: EU925618
  • Sequence Identity:
  • Total samples: 50
  • soil counts: 28
  • aquatic counts: 13
  • animal counts: 6
  • plant counts: 3

Safety information

risk assessment

  • @ref: 8323
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8323
  • description: Bacillus sp. JSM 071004 16S ribosomal RNA gene, partial sequence
  • accession: EU925618
  • length: 1479
  • database: ena
  • NCBI tax ID: 885475

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Salipaludibacillus neizhouensis DSM 19794GCA_002886185scaffoldncbi885475
66792Salipaludibacillus neizhouensis KCTC 13187GCA_003615245contigncbi885475
66792Salipaludibacillus neizhouensis strain DSM 19794885475.3wgspatric885475
66792Salipaludibacillus neizhouensis strain KCTC 13187885475.4wgspatric885475
66792Salipaludibacillus neizhouensis KCTC 131872831550946draftimg885475
66792Salipaludibacillus neizhouensis DSM 197942890125685draftimg885475

GC content

@refGC-contentmethod
832339.8
4362440.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes69.812yes
gram-positiveyes89.214no
anaerobicno98.884yes
aerobicyes80.26no
halophileyes78.776no
spore-formingyes94.477yes
thermophileno99.589yes
glucose-utilyes90.242yes
motileyes89.031no
glucose-fermentno89.998yes

External links

@ref: 8323

culture collection no.: DSM 19794, CCTCC AB 207161, KCTC 13187, JSM 071004

straininfo link

  • @ref: 70944
  • straininfo: 403160

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19643899Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from a sea anemone.Chen YG, Zhang YQ, Wang YX, Liu ZX, Klenk HP, Xiao HD, Tang SK, Cui XL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.009522-02009Animals, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Oceans and Seas, Phylogeny, RNA, Ribosomal, 16S/genetics, Sea Anemones/*microbiology, Seawater/*microbiology, Sodium Chloride/*metabolismMetabolism
Phylogeny27118186Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.Sultanpuram VR, Mothe TInt J Syst Evol Microbiol10.1099/ijsem.0.0011172016Alkalies, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/chemistry, India, Lakes/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29790846Salipaludibacillus halalkaliphilus sp. nov., a moderately haloalkaliphilic bacterium from a coastal-marine wetland.Amoozegar MA, Shahinpei A, Makzum S, Rafieyan S, Moshtaghi Nikou M, Sproer C, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0028142018Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/genetics, Vitamin K 2/analogs & derivatives/chemistry, *WetlandsTranscriptome
Phylogeny30028282Thalassorhabdus alkalitolerans gen. nov., sp. nov., a novel Bacillaceae member isolated from marine sediment.Sultanpuram VR, Mothe TInt J Syst Evol Microbiol10.1099/ijsem.0.0029312018Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylglycerols/analysis, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
8323Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19794)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19794
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29252Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2567228776041
43624Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe10.1099/ijsem.0.001117Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.27118186IJSEM 66: 2747-2753 2016
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70944Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403160.1StrainInfo: A central database for resolving microbial strain identifiers