Strain identifier
BacDive ID: 1293
Type strain:
Species: Salipaludibacillus neizhouensis
Strain history: <- Y.-G. Chen and S.-K. Tang, YIM <- H.-D. Xiao; JSM 071004
NCBI tax ID(s): 885475 (species)
General
@ref: 8323
BacDive-ID: 1293
DSM-Number: 19794
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Salipaludibacillus neizhouensis DSM 19794 is an aerobe, spore-forming, mesophilic bacterium that was isolated from homogenates of sea anemone.
NCBI tax id
- NCBI tax id: 885475
- Matching level: species
strain history
- @ref: 8323
- history: <- Y.-G. Chen and S.-K. Tang, YIM <- H.-D. Xiao; JSM 071004
doi: 10.13145/bacdive1293.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Salipaludibacillus
- species: Salipaludibacillus neizhouensis
- full scientific name: Salipaludibacillus neizhouensis (Chen et al. 2009) Sultanpuram and Mothe 2016
synonyms
- @ref: 20215
- synonym: Bacillus neizhouensis
@ref: 8323
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Salipaludibacillus
species: Salipaludibacillus neizhouensis
full scientific name: Salipaludibacillus neizhouensis (Chen et al. 2009) Sultanpuram and Mothe 2016
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
29252 | positive | 3.0-5.0 µm | 0.5-0.6 µm | rod-shaped | no | |
43624 | positive | rod-shaped | no | |||
69480 | yes | 91.467 | ||||
69480 | positive | 100 |
colony morphology
- @ref: 43624
- colony color: Pale yellow
Culture and growth conditions
culture medium
- @ref: 8323
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l MnSO4 0.01 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8323 | positive | growth | 30 | mesophilic |
29252 | positive | growth | 04-30 | |
29252 | positive | optimum | 25 | mesophilic |
43624 | positive | growth | 4-30 | |
43624 | positive | optimum | 25 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29252 | positive | growth | 6.5-10.0 | alkaliphile |
29252 | positive | optimum | 8.5 | |
43624 | positive | growth | 6.5-10 | |
43624 | positive | optimum | 8.5 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
29252 | aerobe |
43624 | facultative anaerobe |
spore formation
@ref | spore formation | spore description | type of spore | confidence |
---|---|---|---|---|
29252 | yes | |||
43624 | yes | oval or ellipsoidal spores ath the sub-terminal position | endospore | |
69481 | yes | 100 | ||
69480 | yes | 100 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29252 | NaCl | positive | growth | 0.5-10 % |
29252 | NaCl | positive | optimum | 02-04 % |
43624 | NaCl | positive | growth | 0.5-10 %(w/v) |
43624 | NaCl | positive | optimum | 2-4 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29252 | 28757 | fructose | + | carbon source |
29252 | 17234 | glucose | + | carbon source |
29252 | 25017 | leucine | + | carbon source |
29252 | 17306 | maltose | + | carbon source |
29252 | 17992 | sucrose | + | carbon source |
29252 | 27082 | trehalose | + | carbon source |
29252 | 4853 | esculin | + | hydrolysis |
29252 | 17632 | nitrate | + | reduction |
43624 | 16947 | citrate | - | assimilation |
43624 | 27897 | tryptophan | - | energy source |
43624 | 17234 | glucose | - | fermentation |
43624 | casein | - | hydrolysis | |
43624 | 62968 | cellulose | - | hydrolysis |
43624 | 16991 | dna | - | hydrolysis |
43624 | 4853 | esculin | - | hydrolysis |
43624 | 5291 | gelatin | - | hydrolysis |
43624 | 28017 | starch | - | hydrolysis |
43624 | 53424 | tween 20 | + | hydrolysis |
43624 | 17632 | nitrate | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43624 | 15688 | acetoin | no |
43624 | 16136 | hydrogen sulfide | no |
43624 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | methylred-test | voges-proskauer-test | indole test |
---|---|---|---|---|---|
43624 | 17234 | glucose | - | ||
43624 | 15688 | acetoin | - | ||
43624 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29252 | alkaline phosphatase | + | 3.1.3.1 |
29252 | catalase | + | 1.11.1.6 |
29252 | gelatinase | + | |
29252 | cytochrome oxidase | + | 1.9.3.1 |
43624 | catalase | + | 1.11.1.6 |
43624 | cytochrome oxidase | + | 1.9.3.1 |
43624 | urease | - | 3.5.1.5 |
43624 | tryptophan deaminase | - | 4.1.99.1 |
Isolation, sampling and environmental information
isolation
- @ref: 8323
- sample type: homogenates of sea anemone
- geographic location: South China Sea, Neizhou Bay
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
- Cat1: #Host
- Cat2: #Invertebrates (Other)
- Cat3: #Cnidaria (Corals)
taxonmaps
- @ref: 69479
- File name: preview.99_102603.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_144;97_155;98_33125;99_102603&stattab=map
- Last taxonomy: Salipaludibacillus neizhouensis subclade
- 16S sequence: EU925618
- Sequence Identity:
- Total samples: 50
- soil counts: 28
- aquatic counts: 13
- animal counts: 6
- plant counts: 3
Safety information
risk assessment
- @ref: 8323
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 8323
- description: Bacillus sp. JSM 071004 16S ribosomal RNA gene, partial sequence
- accession: EU925618
- length: 1479
- database: ena
- NCBI tax ID: 885475
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Salipaludibacillus neizhouensis DSM 19794 | GCA_002886185 | scaffold | ncbi | 885475 |
66792 | Salipaludibacillus neizhouensis KCTC 13187 | GCA_003615245 | contig | ncbi | 885475 |
66792 | Salipaludibacillus neizhouensis strain DSM 19794 | 885475.3 | wgs | patric | 885475 |
66792 | Salipaludibacillus neizhouensis strain KCTC 13187 | 885475.4 | wgs | patric | 885475 |
66792 | Salipaludibacillus neizhouensis KCTC 13187 | 2831550946 | draft | img | 885475 |
66792 | Salipaludibacillus neizhouensis DSM 19794 | 2890125685 | draft | img | 885475 |
GC content
@ref | GC-content | method |
---|---|---|
8323 | 39.8 | |
43624 | 40.8 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | yes | 69.812 | yes |
gram-positive | yes | 89.214 | no |
anaerobic | no | 98.884 | yes |
aerobic | yes | 80.26 | no |
halophile | yes | 78.776 | no |
spore-forming | yes | 94.477 | yes |
thermophile | no | 99.589 | yes |
glucose-util | yes | 90.242 | yes |
motile | yes | 89.031 | no |
glucose-ferment | no | 89.998 | yes |
External links
@ref: 8323
culture collection no.: DSM 19794, CCTCC AB 207161, KCTC 13187, JSM 071004
straininfo link
- @ref: 70944
- straininfo: 403160
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19643899 | Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from a sea anemone. | Chen YG, Zhang YQ, Wang YX, Liu ZX, Klenk HP, Xiao HD, Tang SK, Cui XL, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.009522-0 | 2009 | Animals, Bacillus/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Oceans and Seas, Phylogeny, RNA, Ribosomal, 16S/genetics, Sea Anemones/*microbiology, Seawater/*microbiology, Sodium Chloride/*metabolism | Metabolism |
Phylogeny | 27118186 | Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov. | Sultanpuram VR, Mothe T | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001117 | 2016 | Alkalies, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/chemistry, India, Lakes/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 29790846 | Salipaludibacillus halalkaliphilus sp. nov., a moderately haloalkaliphilic bacterium from a coastal-marine wetland. | Amoozegar MA, Shahinpei A, Makzum S, Rafieyan S, Moshtaghi Nikou M, Sproer C, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002814 | 2018 | Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/genetics, Vitamin K 2/analogs & derivatives/chemistry, *Wetlands | Transcriptome |
Phylogeny | 30028282 | Thalassorhabdus alkalitolerans gen. nov., sp. nov., a novel Bacillaceae member isolated from marine sediment. | Sultanpuram VR, Mothe T | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002931 | 2018 | Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylglycerols/analysis, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
8323 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19794) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19794 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
29252 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25672 | 28776041 | ||
43624 | Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe | 10.1099/ijsem.0.001117 | Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov. | 27118186 | IJSEM 66: 2747-2753 2016 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
70944 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403160.1 | StrainInfo: A central database for resolving microbial strain identifiers |