Strain identifier
BacDive ID: 12925
Type strain: 
Species: Pseudomonas putida
Strain Designation: MT-I1
Strain history: <- J. H. Wübbeler, Univ. Münster, Inst. Mol. Microbiol. Biotechnol., Germany; MT-I1 <- M. Toups
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 15882
BacDive-ID: 12925
DSM-Number: 21680
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas putida MT-I1 is a mesophilic human pathogen that was isolated from activated sludge of municipal sewage treatment plant Münster-Coerde.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 15882
- history: <- J. H. Wübbeler, Univ. Münster, Inst. Mol. Microbiol. Biotechnol., Germany; MT-I1 <- M. Toups
doi: 10.13145/bacdive12925.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 15882
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: MT-I1
type strain: no
Culture and growth conditions
culture medium
- @ref: 15882
- name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535
- composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
culture temp
- @ref: 15882
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15882 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 15882
- sample type: activated sludge of municipal sewage treatment plant Münster-Coerde
- geographic location: Münster-Coerde
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Waste | #Activated sludge |
| #Engineered | #Waste | #Water treatment plant |
Safety information
risk assessment
- @ref: 15882
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 15882
culture collection no.: DSM 21680
straininfo link
- @ref: 82138
- straininfo: 403811
literature
- topic: Phylogeny
- Pubmed-ID: 19789984
- title: Microbial utilization of the industrial wastewater pollutants 2-ethylhexylthioglycolic acid and iso-octylthioglycolic acid by aerobic gram-negative bacteria.
- authors: Toups M, Wubbeler JH, Steinbuchel A
- journal: Biodegradation
- DOI: 10.1007/s10532-009-9302-y
- year: 2009
- mesh: Aerobiosis, Biodegradation, Environmental, Environmental Microbiology, Gram-Negative Aerobic Bacteria/classification/genetics/isolation & purification/*metabolism, Industrial Waste/*analysis, Molecular Sequence Data, Phylogeny, Sulfur Compounds/*metabolism, Water Pollutants, Chemical/*metabolism
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 15882 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21680) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21680 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82138 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403811.1 | StrainInfo: A central database for resolving microbial strain identifiers |