Strain identifier
BacDive ID: 12921
Type strain: 
Species: Pseudomonas putida
Strain Designation: cl-B
Strain history: ATCC 17453 <-- R. Y. Stanier 77 <-- I. C. Gunsalus C 1-B.
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 12675
BacDive-ID: 12921
DSM-Number: 50198
keywords: 16S sequence, Bacteria, mesophilic, human pathogen
description: Pseudomonas putida cl-B is a mesophilic human pathogen that was isolated from soil.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
| @ref | history |
|---|---|
| 12675 | <- ICPB <- I.C. Gunsalus, C 1-B |
| 67770 | ATCC 17453 <-- R. Y. Stanier 77 <-- I. C. Gunsalus C 1-B. |
doi: 10.13145/bacdive12921.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 12675
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: cl-B
type strain: no
Culture and growth conditions
culture medium
- @ref: 12675
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 12675 | positive | growth | 26 | mesophilic |
| 67770 | positive | growth | 26 | mesophilic |
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 12675 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 12675
- sample type: soil
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_239.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AF094746
- Sequence Identity:
- Total samples: 2133
- soil counts: 368
- aquatic counts: 543
- animal counts: 799
- plant counts: 423
Safety information
risk assessment
- @ref: 12675
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Pseudomonas putida strain ATCC 17453 16S ribosomal RNA gene, partial sequence | AF094746 | 1475 | ena | 303 |
| 20218 | Pseudomonas putida strain ATCC 17453 16S ribosomal RNA gene, partial sequence | JN630891 | 791 | ena | 303 |
External links
@ref: 12675
culture collection no.: DSM 50198, ATCC 17453, ICPB 2563-77, JCM 6157, KCTC 1646
straininfo link
- @ref: 82134
- straininfo: 36664
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 1510672 | Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid. | Selifonov SA | Biochem Biophys Res Commun | 10.1016/s0006-291x(05)81566-9 | 1992 | Adamantane/*analogs & derivatives/isolation & purification/metabolism, Biotransformation, Camphor/*metabolism, Gas Chromatography-Mass Spectrometry, Magnetic Resonance Spectroscopy, Molecular Structure, *Plasmids, Pseudomonas putida/genetics/*metabolism | Enzymology |
| Enzymology | 3003058 | Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli. | Unger BP, Gunsalus IC, Sligar SG | J Biol Chem | S0021-9258(17)36068-4 | 1986 | Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cytochrome P-450 Enzyme System/*genetics, DNA Restriction Enzymes/metabolism, Escherichia coli/*genetics, *Gene Expression Regulation, Isoenzymes/*genetics, Pseudomonas/*enzymology/genetics | Metabolism |
| Metabolism | 3944058 | Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453. | Taylor DG, Trudgill PW | J Bacteriol | 10.1128/jb.165.2.489-497.1986 | 1986 | Apoenzymes/isolation & purification/metabolism, Camphor/*metabolism, Catalysis, Flavin Mononucleotide/metabolism, Flavins/metabolism, Iron/metabolism, Macromolecular Substances, Mixed Function Oxygenases/isolation & purification/*metabolism, Molecular Weight, Multienzyme Complexes/metabolism, NADH, NADPH Oxidoreductases/metabolism, Oxygen/metabolism, *Oxygenases, Pseudomonas/*enzymology, Spectrum Analysis | Enzymology |
| Metabolism | 8253671 | Evidence for autoregulation of camR, which encodes a repressor for the cytochrome P-450cam hydroxylase operon on the Pseudomonas putida CAM plasmid. | Aramaki H, Sagara Y, Hosoi M, Horiuchi T | J Bacteriol | 10.1128/jb.175.24.7828-7833.1993 | 1993 | Base Sequence, Camphor 5-Monooxygenase, Cloning, Molecular, Cytochrome P-450 Enzyme System/biosynthesis/*genetics/metabolism, DNA, Bacterial/metabolism, Deoxyribonuclease I, Escherichia coli/genetics, *Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Genes, Bacterial, Homeostasis, Mixed Function Oxygenases/biosynthesis/*genetics/metabolism, Molecular Sequence Data, Mutagenesis, Site-Directed, Oligodeoxyribonucleotides, *Operon, *Plasmids, Promoter Regions, Genetic, Pseudomonas putida/*enzymology/*genetics, RNA, Messenger/biosynthesis/metabolism, Recombinant Fusion Proteins/biosynthesis/metabolism, Repressor Proteins/*genetics/*metabolism | Enzymology |
| Enzymology | 8334169 | Complete nucleotide sequence of the 5-exo-hydroxycamphor dehydrogenase gene on the CAM plasmid of Pseudomonas putida (ATCC 17453). | Aramaki H, Koga H, Sagara Y, Hosoi M, Horiuchi T | Biochim Biophys Acta | 10.1016/0167-4781(93)90098-x | 1993 | Alcohol Dehydrogenase/chemistry/*genetics, Amino Acid Sequence, Base Sequence, Camphor 5-Monooxygenase, Cytochrome P-450 Enzyme System/chemistry/*genetics, Mixed Function Oxygenases/chemistry/*genetics, Molecular Sequence Data, Pseudomonas putida/*genetics, Sequence Alignment | Genetics |
| Metabolism | 8515237 | Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453. | Jones KH, Smith RT, Trudgill PW | J Gen Microbiol | 10.1099/00221287-139-4-797 | 1993 | Camphor/analogs & derivatives/chemistry/*metabolism, Camphor 5-Monooxygenase, Cross Reactions, Cytochrome P-450 Enzyme System/chemistry/immunology/*metabolism, Isoelectric Point, Mixed Function Oxygenases/chemistry/immunology/*metabolism, Pseudomonas putida/growth & development/*metabolism, Stereoisomerism, Substrate Specificity | |
| Enzymology | 21057946 | Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca. | Schallmey A, den Besten G, Teune IG, Kembaren RF, Janssen DB | Appl Microbiol Biotechnol | 10.1007/s00253-010-2965-9 | 2010 | Actinomycetales/*enzymology, Benzene Derivatives/metabolism, Cloning, Molecular, Electron Transport, Enzyme Stability, Escherichia coli/genetics, Ferredoxins/metabolism, Gene Expression, Hot Temperature, Indoles/metabolism, Mixed Function Oxygenases/chemistry/genetics/isolation & purification/metabolism, NADH, NADPH Oxidoreductases/metabolism, NADPH-Ferrihemoprotein Reductase/chemistry/genetics/*isolation & purification/*metabolism, Phylogeny, Protein Stability, Pseudomonas putida/enzymology, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Sequence Homology, Amino Acid, *Soil Microbiology, Styrenes/metabolism, Sulfides/metabolism, Transition Temperature | Metabolism |
| Enzymology | 22267661 | Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453. | Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC | Appl Environ Microbiol | 10.1128/AEM.07694-11 | 2012 | Amino Acid Sequence, Camphor/*metabolism, Circular Dichroism, Cloning, Molecular/*methods, Crystallography, X-Ray, Cyclopentanes/metabolism, Escherichia coli/enzymology/genetics, Flavin-Adenine Dinucleotide/chemistry/metabolism, Molecular Sequence Data, NADP/chemistry/metabolism, Oxidation-Reduction, Oxygenases/chemistry/*genetics/*metabolism, Pseudomonas putida/*enzymology/genetics, Sequence Analysis, DNA, Substrate Specificity | Metabolism |
| Enzymology | 23524667 | Camphor pathway redux: functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions. | Iwaki H, Grosse S, Bergeron H, Leisch H, Morley K, Hasegawa Y, Lau PC | Appl Environ Microbiol | 10.1128/AEM.03958-12 | 2013 | Acetyl Coenzyme A/metabolism, Amino Acid Sequence, Camphor/*metabolism, Cloning, Molecular, Enzyme Activation, Enzyme Stability, Escherichia coli/genetics/metabolism, FMN Reductase/genetics/isolation & purification/*metabolism, Flavin Mononucleotide/metabolism, Flavin-Adenine Dinucleotide/metabolism, Genes, Bacterial, Molecular Sequence Data, Oxidation-Reduction, Oxygenases/genetics/*metabolism, Plasmids/genetics/metabolism, Pseudomonas putida/*enzymology/genetics | Metabolism |
| Metabolism | 23588837 | Biodegradation of 1-allyloxy-4-propoxybenzene by selected strains of Pseudomonas putida. | Ebrahimi P, Plettner E | Biodegradation | 10.1007/s10532-013-9638-1 | 2013 | Animals, Bacterial Proteins/isolation & purification/*metabolism, Benzene Derivatives/*metabolism, Biodegradation, Environmental, Brassica/parasitology, Camphor 5-Monooxygenase/isolation & purification/*metabolism, Culture Media, Female, Insect Repellents/*metabolism, Kinetics, Lepidoptera/drug effects/physiology, Molecular Docking Simulation, Oviposition/drug effects/physiology, Pseudomonas putida/*enzymology/isolation & purification, *Soil Microbiology, Soil Pollutants/*metabolism, Substrate Specificity | Enzymology |
| Enzymology | 24836624 | Multiple native flavin reductases in camphor-metabolizing Pseudomonas putida NCIMB 10007: functional interaction with two-component diketocamphane monooxygenase isoenzymes. | Willetts A, Kelly DR | Microbiology (Reading) | 10.1099/mic.0.079913-0 | 2014 | Amino Acid Sequence, Camphor/*metabolism, FMN Reductase/genetics/*metabolism, Isoenzymes/chemistry/genetics/*metabolism, Molecular Sequence Data, Oxygenases/chemistry/genetics/*metabolism, Pseudomonas putida/chemistry/*enzymology/genetics/growth & development, Sequence Alignment | Metabolism |
| Metabolism | 25957153 | The production of omega-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli. | Sung C, Jung E, Choi KY, Bae JH, Kim M, Kim J, Kim EJ, Kim PI, Kim BG | Appl Microbiol Biotechnol | 10.1007/s00253-015-6630-1 | 2015 | Biosynthetic Pathways/*genetics, Candida/genetics, Escherichia coli/*genetics/*metabolism, Lactobacillus reuteri/genetics, Marinobacter/genetics, *Metabolic Engineering, Palmitic Acids/*metabolism, Pseudomonas putida/genetics | Biotechnology |
| Metabolism | 30391161 | Chemotaxis by Pseudomonas putida (ATCC 17453) towards camphor involves cytochrome P450cam (CYP101A1). | Balaraman P, Plettner E | Biochim Biophys Acta Gen Subj | 10.1016/j.bbagen.2018.10.018 | 2018 | Camphor/*metabolism/pharmacology, *Chemotaxis/drug effects, Cytochrome P-450 Enzyme System/*metabolism, Molecular Structure, Pseudomonas putida/drug effects/*metabolism | Pathogenicity |
| 30577535 | Characterised Flavin-Dependent Two-Component Monooxygenases from the CAM Plasmid of Pseudomonas putida ATCC 17453 (NCIMB 10007): ketolactonases by Another Name. | Willetts A | Microorganisms | 10.3390/microorganisms7010001 | 2018 | |||
| Enzymology | 31561477 | Conferring the Metabolic Self-Sufficiency of the CAM Plasmid of Pseudomonas putida ATCC 17453: The Key Role of Putidaredoxin Reductase. | Willetts A | Microorganisms | 10.3390/microorganisms7100395 | 2019 | ||
| 34946195 | The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453-An Episodic History and Still Mysterious after 60 Years. | Willetts A | Microorganisms | 10.3390/microorganisms9122593 | 2021 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 12675 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50198) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-50198 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68369 | Automatically annotated from API 20NE | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 82134 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID36664.1 | StrainInfo: A central database for resolving microbial strain identifiers |