Strain identifier

BacDive ID: 12920

Type strain: No

Species: Pseudomonas putida

Strain Designation: Berlin 33/2

Strain history: CIP <- 1989, DSMZ <- G. Bringmann, Germany: strain Berlin 33/2

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 12607

BacDive-ID: 12920

DSM-Number: 50026

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas putida Berlin 33/2 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from channel water.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
12607<- Bringmann; Berlin 33/2
122072CIP <- 1989, DSMZ <- G. Bringmann, Germany: strain Berlin 33/2

doi: 10.13145/bacdive12920.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 12607

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: Berlin 33/2

type strain: no

Morphology

cell morphology

  • @ref: 122072
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 122072
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12607NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35119MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
122072CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
12607positivegrowth28mesophilic
35119positivegrowth30mesophilic
122072positivegrowth10-41
122072nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122072
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122072NaClpositivegrowth0-6 %
122072NaClnogrowth8 %
122072NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12207216947citrate+carbon source
1220724853esculin-hydrolysis
12207217632nitrate-reduction
12207216301nitrite-reduction
12207215882phenol+degradation
12207217632nitrate-respiration

antibiotic resistance

  • @ref: 122072
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12207235581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
122072oxidase+
122072beta-galactosidase-3.2.1.23
122072alcohol dehydrogenase+1.1.1.1
122072gelatinase-
122072DNase-
122072catalase+1.11.1.6
122072tween esterase-
122072lecithinase-
122072lipase-
122072lysine decarboxylase-4.1.1.18
122072ornithine decarboxylase-4.1.1.17
122072protease-
122072tryptophan deaminase-
122072urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122072--++-++-+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
12607---+-+--+++/----++----+
12607---+----++----++-++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122072++-----------------++----------+-----------+++-+++++-++++-+-+++++--+--++++++++-++++++--++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12607channel waterBerlinGermanyDEUEurope
122072Environment, Channel waterBerlinGermanyDEUEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
12607yesyes2Risk group (German classification)
1220721Risk group (French classification)

External links

@ref: 12607

culture collection no.: DSM 50026, CIP 103281

straininfo link

  • @ref: 82133
  • straininfo: 50932

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
9352658Rapid physiological characterization of microorganisms by biosensor technique.Riedel K, Kunze GMicrobiol Res10.1016/S0944-5013(97)80033-X1997*Bacterial Physiological Phenomena, *Biosensing Techniques, Candida/*physiology, Kluyveromyces/*physiology, Pichia/*physiology
10390842A comparison between toxicity tests using single species and a microbial process.van Beelen P, Fleuren-Kemila AKChemosphere10.1016/s0045-6535(98)00559-11999Acetates/metabolism, Aspergillus niger/drug effects, Environmental Pollutants/*toxicity, Pentachlorophenol/*toxicity, Pseudomonas/drug effects, Sensitivity and Specificity, *Soil Microbiology, Streptomyces/drug effects, Toxicity Tests/*methods, *Zinc IsotopesPathogenicityMetabolism
16517220Graphite epoxy composite electrodes modified with bacterial cells.Kirgoz UA, Odaci D, Timur S, Merkoci A, Pazarlioglu N, Telefoncu A, Alegret SBioelectrochemistry10.1016/j.bioelechem.2005.11.0022006Biosensing Techniques/instrumentation/*methods, Electrochemistry, Electrodes, Epoxy Compounds/*chemistry, Graphite/*chemistry, Hydrogen-Ion Concentration, Phenols/analysis/chemistry, Pseudomonas putida/*chemistry/metabolism, Surface Properties, Temperature, Xenobiotics/analysisMetabolism
18969166Detection of phenolic compounds by thick film sensors based on Pseudomonas putida.Timur S, Pazarlioglu N, Pilloton R, Telefoncu ATalanta10.1016/S0039-9140(03)00237-62003
24061176Membrane Changes Associated with Exposure of Pseudomonas putida to Selected Environmental Pollutants and their Possible Roles in Toxicity.Vodovnik M, Bistan M, Zorec M, Marinsek-Logar RActa Chim SlovVodovnik-2012-12012

Reference

@idauthorscataloguedoi/urltitle
12607Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50026)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50026
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35119Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15061
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
82133Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50932.1StrainInfo: A central database for resolving microbial strain identifiers
122072Curators of the CIPCollection of Institut Pasteur (CIP 103281)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103281