Strain identifier
BacDive ID: 12920
Type strain:
Species: Pseudomonas putida
Strain Designation: Berlin 33/2
Strain history: CIP <- 1989, DSMZ <- G. Bringmann, Germany: strain Berlin 33/2
NCBI tax ID(s): 303 (species)
General
@ref: 12607
BacDive-ID: 12920
DSM-Number: 50026
keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen
description: Pseudomonas putida Berlin 33/2 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from channel water.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
@ref | history |
---|---|
12607 | <- Bringmann; Berlin 33/2 |
122072 | CIP <- 1989, DSMZ <- G. Bringmann, Germany: strain Berlin 33/2 |
doi: 10.13145/bacdive12920.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 12607
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: Berlin 33/2
type strain: no
Morphology
cell morphology
- @ref: 122072
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 122072
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
12607 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
35119 | MEDIUM 29- Brain heart agar | yes | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) | |
122072 | CIP Medium 29 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
12607 | positive | growth | 28 | mesophilic |
35119 | positive | growth | 30 | mesophilic |
122072 | positive | growth | 10-41 | |
122072 | no | growth | 5 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 122072
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
122072 | NaCl | positive | growth | 0-6 % |
122072 | NaCl | no | growth | 8 % |
122072 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
122072 | 16947 | citrate | + | carbon source |
122072 | 4853 | esculin | - | hydrolysis |
122072 | 17632 | nitrate | - | reduction |
122072 | 16301 | nitrite | - | reduction |
122072 | 15882 | phenol | + | degradation |
122072 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 122072
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
122072 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
122072 | oxidase | + | |
122072 | beta-galactosidase | - | 3.2.1.23 |
122072 | alcohol dehydrogenase | + | 1.1.1.1 |
122072 | gelatinase | - | |
122072 | DNase | - | |
122072 | catalase | + | 1.11.1.6 |
122072 | tween esterase | - | |
122072 | lecithinase | - | |
122072 | lipase | - | |
122072 | lysine decarboxylase | - | 4.1.1.18 |
122072 | ornithine decarboxylase | - | 4.1.1.17 |
122072 | protease | - | |
122072 | tryptophan deaminase | - | |
122072 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122072 | - | - | + | + | - | + | + | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
12607 | - | - | - | + | - | + | - | - | + | + | +/- | - | - | - | + | + | - | - | - | - | + |
12607 | - | - | - | + | - | - | - | - | + | + | - | - | - | - | + | + | - | + | + | + | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122072 | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | + | + | + | + | + | - | + | + | + | + | - | + | - | + | + | + | + | + | - | - | + | - | - | + | + | + | + | + | + | + | + | - | + | + | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
12607 | channel water | Berlin | Germany | DEU | Europe |
122072 | Environment, Channel water | Berlin | Germany | DEU | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
12607 | yes | yes | 2 | Risk group (German classification) |
122072 | 1 | Risk group (French classification) |
External links
@ref: 12607
culture collection no.: DSM 50026, CIP 103281
straininfo link
- @ref: 82133
- straininfo: 50932
literature
Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
---|---|---|---|---|---|---|---|---|
9352658 | Rapid physiological characterization of microorganisms by biosensor technique. | Riedel K, Kunze G | Microbiol Res | 10.1016/S0944-5013(97)80033-X | 1997 | *Bacterial Physiological Phenomena, *Biosensing Techniques, Candida/*physiology, Kluyveromyces/*physiology, Pichia/*physiology | ||
10390842 | A comparison between toxicity tests using single species and a microbial process. | van Beelen P, Fleuren-Kemila AK | Chemosphere | 10.1016/s0045-6535(98)00559-1 | 1999 | Acetates/metabolism, Aspergillus niger/drug effects, Environmental Pollutants/*toxicity, Pentachlorophenol/*toxicity, Pseudomonas/drug effects, Sensitivity and Specificity, *Soil Microbiology, Streptomyces/drug effects, Toxicity Tests/*methods, *Zinc Isotopes | Pathogenicity | Metabolism |
16517220 | Graphite epoxy composite electrodes modified with bacterial cells. | Kirgoz UA, Odaci D, Timur S, Merkoci A, Pazarlioglu N, Telefoncu A, Alegret S | Bioelectrochemistry | 10.1016/j.bioelechem.2005.11.002 | 2006 | Biosensing Techniques/instrumentation/*methods, Electrochemistry, Electrodes, Epoxy Compounds/*chemistry, Graphite/*chemistry, Hydrogen-Ion Concentration, Phenols/analysis/chemistry, Pseudomonas putida/*chemistry/metabolism, Surface Properties, Temperature, Xenobiotics/analysis | Metabolism | |
18969166 | Detection of phenolic compounds by thick film sensors based on Pseudomonas putida. | Timur S, Pazarlioglu N, Pilloton R, Telefoncu A | Talanta | 10.1016/S0039-9140(03)00237-6 | 2003 | |||
24061176 | Membrane Changes Associated with Exposure of Pseudomonas putida to Selected Environmental Pollutants and their Possible Roles in Toxicity. | Vodovnik M, Bistan M, Zorec M, Marinsek-Logar R | Acta Chim Slov | Vodovnik-2012-1 | 2012 |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
12607 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50026) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-50026 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
35119 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15061 | ||
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
82133 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID50932.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
122072 | Curators of the CIP | Collection of Institut Pasteur (CIP 103281) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103281 |