Strain identifier

BacDive ID: 12919

Type strain: No

Species: Pseudomonas putida

Strain Designation: 50-3-3, IBPM B-20

Strain history: CIP <- 1963, O. Lysenko, Inst. Biol., Pragues

NCBI tax ID(s): 303 (species)

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General

@ref: 12585

BacDive-ID: 12919

DSM-Number: 46608

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas putida 50-3-3 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from fall webworm Hyphantria cunea.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

@refhistory
12585<- IMET <- CCEB <- O. Lysenko; 50-3-3
334201963, O. Lysenko, Inst. Biol., Pragues
122070CIP <- 1963, O. Lysenko, Inst. Biol., Pragues

doi: 10.13145/bacdive12919.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 12585

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: 50-3-3, IBPM B-20

type strain: no

Morphology

cell morphology

  • @ref: 122070
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 122070
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12585TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
12585ORGANIC MEDIUM 79 (DSMZ Medium 426)yeshttps://mediadive.dsmz.de/medium/426Name: ORGANIC MEDIUM 79 (DSMZ Medium 426) Composition: Agar 15.0 g/l Peptone 10.0 g/l Dextrose 10.0 g/l NaCl 6.0 g/l Yeast extract 2.0 g/l Casein peptone 2.0 g/l Distilled water
33420MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122070CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
12585positivegrowth30mesophilic
33420positivegrowth30mesophilic
122070positivegrowth10-41
122070nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122070
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122070NaClpositivegrowth0-6 %
122070NaClnogrowth8 %
122070NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12207016947citrate+carbon source
1220704853esculin-hydrolysis
12207017632nitrate-reduction
12207016301nitrite-reduction
12207017632nitrate-respiration
6837418257ornithine-degradation
6837429016arginine+hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose-builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate+assimilation
6837427897tryptophan-energy source
6837417634D-glucose-builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

antibiotic resistance

  • @ref: 122070
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleno
6836935581indoleno
12207035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6837435581indole-
6836935581indole-

enzymes

@refvalueactivityec
68374L-aspartate arylamidase+3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase-3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase-3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase+
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase+3.5.3.6
68374ornithine decarboxylase-4.1.1.17
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
122070oxidase+
122070beta-galactosidase-3.2.1.23
122070alcohol dehydrogenase+1.1.1.1
122070gelatinase-
122070amylase-
122070DNase-
122070caseinase-3.4.21.50
122070catalase+1.11.1.6
122070tween esterase-
122070lecithinase-
122070lipase-
122070lysine decarboxylase-4.1.1.18
122070ornithine decarboxylase-4.1.1.17
122070tryptophan deaminase-
122070urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44229C12:02.112
    44229C16:02716
    44229C10:0 3OH5.311.423
    44229C12:0 2OH6.813.178
    44229C12:0 3OH4.813.455
    44229C16:1 ω7c34.415.819
    44229C17:0 CYCLO3.216.888
    44229C18:1 ω7c /12t/9t16.417.824
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122070--++-+--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
12585---+----+-----++-++++

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44229-+-----++------+---------------+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122070++--+-------------++-----------+-----------++--+++++-++++---++++++-+--++++++++-++++++-+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typehost speciessampling date
12585fall webworm Hyphantria cuneaHyphantria cunea
44229Fall webworm,Hyphantria cunea1959

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Insecta

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
12585yesyes2Risk group (German classification)
1220701Risk group (French classification)

External links

@ref: 12585

culture collection no.: DSM 46608, CCEB 520, CIP 63.23, IMET 10411, NCIB 9887, NRRL B-208, VKM 901, CCUG 554 B, LMG 2259

straininfo link

  • @ref: 82132
  • straininfo: 4219

Reference

@idauthorscataloguedoi/urltitle
12585Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46608)https://www.dsmz.de/collection/catalogue/details/culture/DSM-46608
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33420Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10029
44229Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 554 B)https://www.ccug.se/strain?id=554
68369Automatically annotated from API 20NE
68374Automatically annotated from API ID32E
68382Automatically annotated from API zym
82132Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4219.1StrainInfo: A central database for resolving microbial strain identifiers
122070Curators of the CIPCollection of Institut Pasteur (CIP 63.23)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2063.23