Strain identifier
BacDive ID: 12919
Type strain:
Species: Pseudomonas putida
Strain Designation: 50-3-3, IBPM B-20
Strain history: CIP <- 1963, O. Lysenko, Inst. Biol., Pragues
NCBI tax ID(s): 303 (species)
General
@ref: 12585
BacDive-ID: 12919
DSM-Number: 46608
keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen
description: Pseudomonas putida 50-3-3 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from fall webworm Hyphantria cunea.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
@ref | history |
---|---|
12585 | <- IMET <- CCEB <- O. Lysenko; 50-3-3 |
33420 | 1963, O. Lysenko, Inst. Biol., Pragues |
122070 | CIP <- 1963, O. Lysenko, Inst. Biol., Pragues |
doi: 10.13145/bacdive12919.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 12585
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: 50-3-3, IBPM B-20
type strain: no
Morphology
cell morphology
- @ref: 122070
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 122070
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
12585 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
12585 | ORGANIC MEDIUM 79 (DSMZ Medium 426) | yes | https://mediadive.dsmz.de/medium/426 | Name: ORGANIC MEDIUM 79 (DSMZ Medium 426) Composition: Agar 15.0 g/l Peptone 10.0 g/l Dextrose 10.0 g/l NaCl 6.0 g/l Yeast extract 2.0 g/l Casein peptone 2.0 g/l Distilled water |
33420 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
122070 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
12585 | positive | growth | 30 | mesophilic |
33420 | positive | growth | 30 | mesophilic |
122070 | positive | growth | 10-41 | |
122070 | no | growth | 5 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 122070
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
122070 | NaCl | positive | growth | 0-6 % |
122070 | NaCl | no | growth | 8 % |
122070 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
122070 | 16947 | citrate | + | carbon source |
122070 | 4853 | esculin | - | hydrolysis |
122070 | 17632 | nitrate | - | reduction |
122070 | 16301 | nitrite | - | reduction |
122070 | 17632 | nitrate | - | respiration |
68374 | 18257 | ornithine | - | degradation |
68374 | 29016 | arginine | + | hydrolysis |
68374 | 25094 | lysine | - | degradation |
68374 | 16199 | urea | - | hydrolysis |
68374 | 18403 | L-arabitol | - | builds acid from |
68374 | 18024 | D-galacturonic acid | - | builds acid from |
68374 | Potassium 5-ketogluconate | - | builds acid from | |
68374 | 16899 | D-mannitol | - | builds acid from |
68374 | 17306 | maltose | - | builds acid from |
68374 | 15963 | ribitol | - | builds acid from |
68374 | 18394 | palatinose | - | builds acid from |
68374 | 15792 | malonate | + | assimilation |
68374 | 27897 | tryptophan | - | energy source |
68374 | 17634 | D-glucose | - | builds acid from |
68374 | 17992 | sucrose | - | builds acid from |
68374 | 30849 | L-arabinose | - | builds acid from |
68374 | 18333 | D-arabitol | - | builds acid from |
68374 | 27082 | trehalose | - | builds acid from |
68374 | 62345 | L-rhamnose | - | builds acid from |
68374 | 17268 | myo-inositol | - | builds acid from |
68374 | 17057 | cellobiose | - | builds acid from |
68374 | 30911 | sorbitol | - | builds acid from |
antibiotic resistance
- @ref: 122070
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68374 | 35581 | indole | no |
68369 | 35581 | indole | no |
122070 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test |
---|---|---|---|
68374 | 35581 | indole | - |
68369 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68374 | L-aspartate arylamidase | + | 3.4.11.21 |
68374 | alpha-maltosidase | - | |
68374 | alpha-galactosidase | - | 3.2.1.22 |
68374 | alpha-glucosidase | - | 3.2.1.20 |
68374 | beta-galactosidase | - | 3.2.1.23 |
68374 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68374 | beta-glucuronidase | - | 3.2.1.31 |
68374 | beta-glucosidase | - | 3.2.1.21 |
68374 | lipase | + | |
68374 | urease | - | 3.5.1.5 |
68374 | lysine decarboxylase | - | 4.1.1.18 |
68374 | arginine dihydrolase | + | 3.5.3.6 |
68374 | ornithine decarboxylase | - | 4.1.1.17 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
122070 | oxidase | + | |
122070 | beta-galactosidase | - | 3.2.1.23 |
122070 | alcohol dehydrogenase | + | 1.1.1.1 |
122070 | gelatinase | - | |
122070 | amylase | - | |
122070 | DNase | - | |
122070 | caseinase | - | 3.4.21.50 |
122070 | catalase | + | 1.11.1.6 |
122070 | tween esterase | - | |
122070 | lecithinase | - | |
122070 | lipase | - | |
122070 | lysine decarboxylase | - | 4.1.1.18 |
122070 | ornithine decarboxylase | - | 4.1.1.17 |
122070 | tryptophan deaminase | - | |
122070 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 44229 C12:0 2.1 12 44229 C16:0 27 16 44229 C10:0 3OH 5.3 11.423 44229 C12:0 2OH 6.8 13.178 44229 C12:0 3OH 4.8 13.455 44229 C16:1 ω7c 34.4 15.819 44229 C17:0 CYCLO 3.2 16.888 44229 C18:1 ω7c /12t/9t 16.4 17.824 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122070 | - | - | + | + | - | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
12585 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | + | + | - | + | + | + | + |
API ID32E
@ref | ODC | ADH Arg | LDC Lys | URE | LARL | GAT | 5KG | LIP | RP | beta GLU | MAN | MAL | ADO | PLE | beta GUR | MNT | IND | beta NAG | beta GAL | GLU | SAC | LARA | DARL | alpha GLU | alpha GAL | TRE | RHA | INO | CEL | SOR | alphaMAL | AspA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
44229 | - | + | - | - | - | - | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122070 | + | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | + | + | + | + | + | - | + | + | + | + | - | - | - | + | + | + | + | + | + | - | + | - | - | + | + | + | + | + | + | + | + | - | + | + | + | + | + | + | - | + | - | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | sampling date |
---|---|---|---|
12585 | fall webworm Hyphantria cunea | Hyphantria cunea | |
44229 | Fall webworm,Hyphantria cunea | 1959 |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
12585 | yes | yes | 2 | Risk group (German classification) |
122070 | 1 | Risk group (French classification) |
External links
@ref: 12585
culture collection no.: DSM 46608, CCEB 520, CIP 63.23, IMET 10411, NCIB 9887, NRRL B-208, VKM 901, CCUG 554 B, LMG 2259
straininfo link
- @ref: 82132
- straininfo: 4219
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
12585 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46608) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46608 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
33420 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/10029 | ||
44229 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 554 B) | https://www.ccug.se/strain?id=554 | |
68369 | Automatically annotated from API 20NE | |||
68374 | Automatically annotated from API ID32E | |||
68382 | Automatically annotated from API zym | |||
82132 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID4219.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
122070 | Curators of the CIP | Collection of Institut Pasteur (CIP 63.23) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2063.23 |