Strain identifier

BacDive ID: 12911

Type strain: No

Species: Pseudomonas putida

Strain Designation: GS1

Strain history: <- A. E. Mars; GS1

NCBI tax ID(s): 303 (species)

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General

@ref: 4663

BacDive-ID: 12911

DSM-Number: 12264

keywords: Bacteria, mesophilic, human pathogen

description: Pseudomonas putida GS1 is a mesophilic human pathogen that was isolated from sludge of wastewater treatment system.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 4663
  • history: <- A. E. Mars; GS1

doi: 10.13145/bacdive12911.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 4663

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: GS1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4663LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
4663REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

culture temp

  • @ref: 4663
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

compound production

  • @ref: 4663
  • compound: perillic acid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4663---+----+-----++-++++

Isolation, sampling and environmental information

isolation

  • @ref: 4663
  • sample type: sludge of wastewater treatment system
  • geographic location: Wageningen
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Mud (Sludge)

Safety information

risk assessment

  • @ref: 4663
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 4663

culture collection no.: DSM 12264

straininfo link

  • @ref: 82124
  • straininfo: 49261

literature

  • topic: Metabolism
  • Pubmed-ID: 25471523
  • title: De novo production of the monoterpenoid geranic acid by metabolically engineered Pseudomonas putida.
  • authors: Mi J, Becher D, Lubuta P, Dany S, Tusch K, Schewe H, Buchhaupt M, Schrader J
  • journal: Microb Cell Fact
  • DOI: 10.1186/s12934-014-0170-8
  • year: 2014
  • mesh: Escherichia coli/genetics/metabolism, *Metabolic Engineering, Monoterpenes/*metabolism, Pseudomonas putida/*genetics/*metabolism, Saccharomyces cerevisiae/genetics/metabolism, Terpenes/*metabolism
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitle
4663Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12264)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12264
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
82124Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49261.1StrainInfo: A central database for resolving microbial strain identifiers