Strain identifier
BacDive ID: 12911
Type strain:
Species: Pseudomonas putida
Strain Designation: GS1
Strain history: <- A. E. Mars; GS1
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 4663
BacDive-ID: 12911
DSM-Number: 12264
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas putida GS1 is a mesophilic human pathogen that was isolated from sludge of wastewater treatment system.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 4663
- history: <- A. E. Mars; GS1
doi: 10.13145/bacdive12911.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 4663
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: GS1
type strain: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4663 | LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) | yes | https://mediadive.dsmz.de/medium/381 | Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water |
4663 | REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) | yes | https://mediadive.dsmz.de/medium/220a | Name: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water |
culture temp
- @ref: 4663
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
compound production
- @ref: 4663
- compound: perillic acid
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
4663 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 4663
- sample type: sludge of wastewater treatment system
- geographic location: Wageningen
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Wastewater |
#Environmental | #Terrestrial | #Mud (Sludge) |
Safety information
risk assessment
- @ref: 4663
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 4663
culture collection no.: DSM 12264
straininfo link
- @ref: 82124
- straininfo: 49261
literature
- topic: Metabolism
- Pubmed-ID: 25471523
- title: De novo production of the monoterpenoid geranic acid by metabolically engineered Pseudomonas putida.
- authors: Mi J, Becher D, Lubuta P, Dany S, Tusch K, Schewe H, Buchhaupt M, Schrader J
- journal: Microb Cell Fact
- DOI: 10.1186/s12934-014-0170-8
- year: 2014
- mesh: Escherichia coli/genetics/metabolism, *Metabolic Engineering, Monoterpenes/*metabolism, Pseudomonas putida/*genetics/*metabolism, Saccharomyces cerevisiae/genetics/metabolism, Terpenes/*metabolism
- topic2: Biotechnology
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
4663 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12264) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12264 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
68369 | Automatically annotated from API 20NE | |||
82124 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49261.1 | StrainInfo: A central database for resolving microbial strain identifiers |