Strain identifier

BacDive ID: 1290

Type strain: Yes

Species: Priestia aryabhattai

Strain Designation: B8W22

Strain history: S. Shivaji B8W22.

NCBI tax ID(s): 412384 (species)

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General

@ref: 15484

BacDive-ID: 1290

DSM-Number: 21047

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Priestia aryabhattai B8W22 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from cryogenic tube used for collecting air from an altitude of 40–41.4 km.

NCBI tax id

  • NCBI tax id: 412384
  • Matching level: species

strain history

@refhistory
15484<- S. Shivaji, Centre for Cellular and Molecular Biology, Hyderabad, India; B8W22
67770S. Shivaji B8W22.

doi: 10.13145/bacdive1290.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Priestia
  • species: Priestia aryabhattai
  • full scientific name: Priestia aryabhattai (Shivaji et al. 2009) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus aryabhattai

@ref: 15484

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus aryabhattai

full scientific name: Bacillus aryabhattai Shivaji et al. 2009

strain designation: B8W22

type strain: yes

Morphology

cell morphology

  • @ref: 22892
  • gram stain: positive
  • cell shape: rod-shaped

colony morphology

  • @ref: 22892
  • colony size: 5 - 8 mm

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
22892positivemaximum37mesophilic
22892positiveminimum10psychrophilic
22892positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 22892
  • spore description: ellipsoidal, central
  • type of spore: endospore
  • spore formation: yes

compound production

@refcompound
22891Acetoin
22892Acetoin

halophily

  • @ref: 22892
  • salt: NaCl
  • growth: positive
  • tested relation: resistant
  • concentration: 11.6 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2289118305arbutin+growth
22891casein+hydrolysis
2289117057cellobiose+growth
2289117108D-arabinose+growth
2289115824D-fructose-hydrolysis
2289128847D-fucose+growth
2289112936D-galactose+growth
2289116899D-mannitol+growth
2289117924D-sorbitol-growth
2289165327D-xylose+growth
2289116813galactitol-growth
2289117268myo-inositol+growth
2289115443inulin-growth
2289117716lactose+growth
228916731melezitose+growth
2289128053melibiose+growth
22891320061methyl alpha-D-glucopyranoside+growth
22891506227N-acetyl-D-glucosamine+growth
2289117814salicin+growth
22892168082-dehydro-D-gluconate+growth
22892581435-dehydro-D-gluconate+growth
2289230089acetate+growth
2289215963ribitol+builds acid from
2289215963ribitol+growth
2289227613amygdalin+growth
2289218305arbutin-growth
22892casein+hydrolysis
2289217057cellobiose+builds acid from
2289217057cellobiose+growth
2289262968cellulose-growth
2289216947citrate-growth
2289216919creatine+growth
2289217108D-arabinose+builds acid from
2289217108D-arabinose+growth
2289215824D-fructose+growth
2289212936D-galactose+growth
2289217634D-glucose+growth
2289215748D-glucuronate-growth
2289216899D-mannitol+growth
2289216024D-mannose+growth
2289216988D-ribose+builds acid from
2289216988D-ribose+growth
2289217924D-sorbitol+growth
2289265327D-xylose+builds acid from
2289265327D-xylose+growth
2289252071dextran-growth
2289223652dextrin-growth
2289216813galactitol-growth
2289217113erythritol+builds acid from
2289217113erythritol+growth
228924853esculin+growth
228924853esculin+hydrolysis
2289215740formate+growth
2289228757fructose+builds acid from
2289229806fumarate+growth
2289228260galactose+builds acid from
2289228260galactose+growth
2289224265gluconate+/-growth
2289217234glucose+builds acid from
2289217754glycerol+builds acid from
2289217754glycerol+growth
2289215428glycine+growth
2289228087glycogen-growth
2289224684hydroxybutanoic acid-growth
2289217268myo-inositol+builds acid from
2289215443inulin-builds acid from
2289215443inulin-growth
2289216977L-alanine+growth
2289230849L-arabinose+builds acid from
2289230849L-arabinose+growth
2289216467L-arginine+growth
2289217196L-asparagine+growth
2289229991L-aspartate-growth
2289217561L-cysteine-growth
2289218287L-fucose-growth
2289229985L-glutamate+growth
2289218050L-glutamine-growth
2289215971L-histidine-growth
2289217191L-isoleucine+/-growth
2289215603L-leucine+growth
2289218019L-lysine+growth
2289216643L-methionine+growth
2289215729L-ornithine+growth
2289217295L-phenylalanine-growth
2289217203L-proline-growth
2289217115L-serine+growth
2289217266L-sorbose+growth
2289216857L-threonine+growth
2289216828L-tryptophan+growth
2289217895L-tyrosine-growth
2289265328L-xylose+builds acid from
2289265328L-xylose+growth
2289224996lactate+growth
2289217716lactose+builds acid from
2289217716lactose+growth
2289225115malate-growth
2289215792malonate+growth
2289217306maltose+builds acid from
2289217306maltose+growth
2289229864mannitol+builds acid from
2289237684mannose+builds acid from
228926731melezitose+growth
2289228053melibiose+builds acid from
2289228053melibiose+growth
2289237657methyl D-glucoside-growth
2289255507methyl alpha-D-galactoside+growth
2289243943methyl alpha-D-mannoside+growth
2289217540methyl beta-D-galactoside-growth
2289217268myo-inositol+growth
22892506227N-acetyl-D-glucosamine+growth
22892309162-oxoglutarate-builds acid from
22892309162-oxoglutarate-growth
2289246793polyethylene glycol+growth
2289217272propionate+growth
2289215361pyruvate-growth
2289216634raffinose+growth
2289226546rhamnose-builds acid from
2289226546rhamnose+growth
2289217814salicin-growth
2289263675sodium succinate-growth
2289230911sorbitol+builds acid from
2289228017starch+growth
2289228017starch+hydrolysis
2289217992sucrose-builds acid from
2289217992sucrose+growth
2289247869thioglycolate-growth
2289227082trehalose+builds acid from
2289227082trehalose+growth
2289231011valerate-growth
2289217151xylitol+growth
2289217632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
228912676amoxicillinyesyes15 µg (disc)
2289117076streptomycinyesyes25 µg (disc)
2289127902tetracyclineyesyes30 µg (disc)
228916104kanamycinyesyes30 µg (disc)
2289117698chloramphenicolyesyes30 µg (disc)
228919215spectinomycinyesyes100 µg (disc)
2289237943colistinyesyes10 µg (disc)
22892100246norfloxacinyesyes10 µg (disc)
2289228864tobramycinyesyes15 µg (disc)
22892116278lomefloxacinyesyes30 µg (disc)
228922637amikacinyesyes30 µg (disc)
2289248844roxithromycinyesyes15 µg (disc)
22892100241ciprofloxacinyesyes30 µg (disc)
2289271415nitrofurantoinyesyes300 µg (disc)
228923493cefoperazoneyesyes75 µg (disc)
2289228001vancomycinyesyes30 µg (disc)
228926472lincomycinyesyes15 µg (disc)
22892204928cefotaximeyesyes30 µg (disc)
228926104kanamycinyesyes30 µg (disc)
2289228368novobiocinyesyes30 µg (disc)
2289217698chloramphenicolyesyes30 µg (disc)
2289228971ampicillinyesyes25 µg (disc)
2289227902tetracyclineyesyes30 µg (disc)
2289228669bacitracinyesyes10 µg (disc)
2289217833gentamicinyesyes30 µg (disc)
228928309polymyxin byesyes50 µg (disc)
2289216869oleandomycinyesyes15 µg (disc)
228929215spectinomycinyesyes100 µg (disc)
2289228077rifampicinyesyes30 µg (disc)
2289248923erythromycinyesyes15 µg (disc)
228923393carbenicillinyesyes100 µg (disc)
2289217334penicillinyesyes10 µg (disc)
228923515cefuroximeyesyes30 µg (disc)
228922676amoxicillinyesyes15 µg (disc)
2289250845doxycyclineyesyes10 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2289116136hydrogen sulfideno
2289115688acetoinyes
2289235581indoleno
2289215688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
2289115688acetoin+
2289215688acetoin+

enzymes

@refvalueactivityec
22891alpha-chymotrypsin+3.4.21.1
22891beta-glucuronidase-3.2.1.31
22891cytochrome oxidase+1.9.3.1
22892arginine dihydrolase-3.5.3.6
22892gelatinase+
22892lysine decarboxylase+4.1.1.18
22892ornithine decarboxylase+4.1.1.17
22892cytochrome oxidase+1.9.3.1
22892tryptophan deaminase+4.1.99.1
22892lipase (Tween 80)-
22892urease+3.5.1.5
22892caseinase+3.4.21.50
22892arginine decarboxylase-4.1.1.19

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
15484cryogenic tube used for collecting air from an altitude of 40–41.4 kmIndiaINDAsia
67770Cryogenic tube used for collecting an air sample from an altitude of 40-41.4 km

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Engineered product
#Environmental#Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_514.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_136;97_147;98_164;99_514&stattab=map
  • Last taxonomy: Priestia
  • 16S sequence: EF114313
  • Sequence Identity:
  • Total samples: 18607
  • soil counts: 7776
  • aquatic counts: 1712
  • animal counts: 6012
  • plant counts: 3107

Safety information

risk assessment

  • @ref: 15484
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15484
  • description: Bacillus aryabhattai strain B8W22 16S ribosomal RNA gene, partial sequence
  • accession: EF114313
  • length: 1533
  • database: ena
  • NCBI tax ID: 1358420

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus aryabhattai B8W221358420.3wgspatric1358420
67770Priestia aryabhattai B8W22GCA_900101665scaffoldncbi1358420
67770Priestia aryabhattai B8W22GCA_000956595contigncbi1358420

GC content

  • @ref: 15484
  • GC-content: 38

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.945no
gram-positiveyes89.768yes
anaerobicno98.002no
halophileno58.302no
spore-formingyes93.457no
thermophileno99.105yes
glucose-utilyes92.433yes
flagellatedyes87.527no
aerobicyes85.843no
glucose-fermentno85.931no

External links

@ref: 15484

culture collection no.: DSM 21047, JCM 13839, MTCC 7755

straininfo link

  • @ref: 70942
  • straininfo: 361089

literature

  • topic: Phylogeny
  • Pubmed-ID: 34226954
  • title: Priestia veravalensis sp. nov., isolated from coastal sample.
  • authors: Wagh VS, Ram H, Dastager SG
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-021-02418-z
  • year: 2021
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial, *Fatty Acids, Nucleic Acid Hybridization, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15484Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21047)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21047
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22891Jun Xi, Lin-Yan He, Zhi Huang, Xia-Fang Sheng10.1099/ijs.0.061929-0Bacillus qingshengii sp. nov., a rock-weathering bacterium isolated from weathered rock surfaceIJSEM 64: 2473-2479 201424801156
22892S. Shivaji, Preeti Chaturvedi, Zareena Begum, Pavan Kumar Pindi, R. Manorama, D. Ananth Padmanaban, Yogesh S. Shouche, Shrikant Pawar, Parag Vaishampayan, C. B. S. Dutt, G. N. Datta, R. K. Manchanda, U. R. Rao, P. M. Bhargava, J. V. Narlikar10.1099/ijs.0.002527-0Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphereIJSEM 59: 2977-2986 200919643890
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70942Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID361089.1StrainInfo: A central database for resolving microbial strain identifiers