Strain identifier
BacDive ID: 12894
Type strain: 
Species: Pseudomonas putida
Strain Designation: CLB250
Strain history: <- K.H. Engesser; CLB250
NCBI tax ID(s): 303 (species)
version 9.2 (current version)
General
@ref: 1967
BacDive-ID: 12894
DSM-Number: 5232
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas putida CLB250 is a mesophilic human pathogen that was isolated from sewage.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 1967
- history: <- K.H. Engesser; CLB250
doi: 10.13145/bacdive12894.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Arthrobacter siderocapsulatus 20215 Bacillus putidus
@ref: 1967
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: CLB250
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1967 | MINERAL MEDIUM WITH 2-CHLOROBENZOATE (DSMZ Medium 457a) | yes | https://mediadive.dsmz.de/medium/457a | Name: MINERAL MEDIUM WITH 2-CHLOROBENZOATE (DSMZ Medium 457a) Composition: Na2HPO4 2.41584 g/l KH2PO4 1.50495 g/l 2-Chlorobenzoic acid 0.775248 g/l (NH4)2SO4 0.49505 g/l MgSO4 x 7 H2O 0.19802 g/l CaCl2 x 2 H2O 0.049505 g/l EDTA 0.00495049 g/l FeSO4 x 7 H2O 0.0019802 g/l H3BO3 0.00029703 g/l CoCl2 x 6 H2O 0.00019802 g/l ZnSO4 x 7 H2O 9.90099e-05 g/l MnCl2 x 4 H2O 2.9703e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l NiCl2 x 6 H2O 1.9802e-05 g/l CuCl2 x 2 H2O 9.90099e-06 g/l Distilled water |
| 1967 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
culture temp
- @ref: 1967
- growth: positive
- type: growth
- temperature: 30
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1967 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 1967
- sample type: sewage
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Wastewater
Safety information
risk assessment
- @ref: 1967
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 1967
culture collection no.: DSM 5232
straininfo link
- @ref: 82107
- straininfo: 47908
literature
- topic: Metabolism
- Pubmed-ID: 2777062
- title: Degradation of 2-bromo-, 2-chloro- and 2-fluorobenzoate by Pseudomonas putida CLB 250.
- authors: Engesser KH, Schulte P
- journal: FEMS Microbiol Lett
- DOI: 10.1016/0378-1097(89)90497-7
- year: 1989
- mesh: *Benzoates/*metabolism, Biodegradation, Environmental, Bromobenzoates/*metabolism, Catechols/biosynthesis, Chlorobenzoates/*metabolism, Pseudomonas/enzymology/*metabolism
- topic2: Enzymology
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1967 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5232) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5232 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82107 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47908.1 | StrainInfo: A central database for resolving microbial strain identifiers |