Strain identifier

BacDive ID: 12882

Type strain: No

Species: Pseudomonas putida

Strain Designation: MT15, pWW15

NCBI tax ID(s): 303 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1523

BacDive-ID: 12882

DSM-Number: 3936

keywords: Bacteria

description: Pseudomonas putida MT15 is a bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

doi: 10.13145/bacdive12882.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 1523

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudomonadales

family: Micrococcaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: MT15, pWW15

type strain: no

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase+3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
1523-++-+---------------+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
1523+--+----++++--++-++++
1523+--++---++++--++-+++

Safety information

risk assessment

  • @ref: 1523
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 1523

culture collection no.: DSM 3936, NCIB 12184

straininfo link

  • @ref: 82095
  • straininfo: 112632

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism2168927Physical map of the aromatic amine and m-toluate catabolic plasmid pTDN1 in Pseudomonas putida: location of a unique meta-cleavage pathway.Saint CP, McClure NC, Venables WAJ Gen Microbiol10.1099/00221287-136-4-6151990Aniline Compounds/*metabolism, *Benzoates/*metabolism, Catechol 2,3-Dioxygenase, DNA Transposable Elements, *Dioxygenases, Genes, Bacterial, Hydrolysis, Mutation, Oxygenases/*genetics, Plasmids, Pseudomonas/*genetics/growth & development/metabolism, Restriction Mapping, Toluidines/metabolism
Enzymology4008443TOL plasmid pWW15 contains two nonhomologous, independently regulated catechol 2,3-oxygenase genes.Keil H, Lebens MR, Williams PAJ Bacteriol10.1128/jb.163.1.248-255.19851985Catechol 2,3-Dioxygenase, Chromosome Deletion, Chromosome Mapping, Cloning, Molecular, *Dioxygenases, Gene Expression Regulation, Genes, *Genes, Bacterial, Mutation, Oxygenases/*genetics, *Plasmids, Pseudomonas/*genetics, Toluene/*metabolismMetabolism
Enzymology9451836Cloning and sequence analysis of a catechol 2,3-dioxygenase gene from the nitrobenzene-degrading strain Comamonas sp JS765.Parales RE, Ontl TA, Gibson DTJ Ind Microbiol Biotechnol10.1038/sj.jim.29004201997Amino Acid Sequence, Base Sequence, Biodegradation, Environmental, Catechol 2,3-Dioxygenase, Cloning, Molecular, *Dioxygenases, Gram-Negative Aerobic Rods and Cocci/*enzymology, Molecular Sequence Data, Nitrobenzenes/*metabolism, Oxygenases/*geneticsGenetics

Reference

@idauthorscataloguedoi/urltitle
1523Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3936)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3936
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
82095Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID112632.1StrainInfo: A central database for resolving microbial strain identifiers