Strain identifier
BacDive ID: 12880
Type strain:
Species: Pseudomonas putida
Strain Designation: mt-2 PaW1, pWWO
Strain history: ATCC 33015 <-- P. A. Williams mt-2 <-- G. D. Hegeman.
NCBI tax ID(s): 303 (species)
General
@ref: 1518
BacDive-ID: 12880
DSM-Number: 3931
keywords: 16S sequence, Bacteria, mesophilic
description: Pseudomonas putida mt-2 PaW1 is a mesophilic bacterium that was isolated from Soil using benzoate as a major carbon source.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 67770
- history: ATCC 33015 <-- P. A. Williams mt-2 <-- G. D. Hegeman.
doi: 10.13145/bacdive12880.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 1518
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Pseudomonadales
family: Micrococcaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: mt-2 PaW1, pWWO
type strain: no
Culture and growth conditions
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
44546 | positive | growth | 26 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
44546 | - | - | + | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1518 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | + |
1518 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | + |
1518 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | + |
44546 | - | - | - | + | - | - | - | - | + | - | + | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 44546
- sample type: Soil using benzoate as a major carbon source
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_239.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AB598738
- Sequence Identity:
- Total samples: 2133
- soil counts: 368
- aquatic counts: 543
- animal counts: 799
- plant counts: 423
Safety information
risk assessment
- @ref: 1518
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Pseudomonas putida (strain JCM 6156 (ATCC 33015)) gene for 16S ribosomal RNA, partial sequence | D37924 | 1329 | ena | 303 |
67770 | Pseudomonas putida gene for 16S rRNA, partial sequence, strain: JCM 6156 | AB598738 | 1459 | ena | 303 |
External links
@ref: 1518
culture collection no.: DSM 3931, ATCC 33015, NCIB 12182, CCUG 2480, ATCC 23973, JCM 6156, BCRC 10460
straininfo link
@ref | straininfo |
---|---|
82092 | 92520 |
82093 | 112627 |
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 3794647 | Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines. | McClure NC, Venables WA | J Gen Microbiol | 10.1099/00221287-132-8-2209 | 1986 | *Adaptation, Physiological, Amines/*metabolism, Biodegradation, Environmental, Plasmids, Pseudomonas/*growth & development, Substrate Specificity, Toluene/metabolism, Toluidines/metabolism | |
Phylogeny | 11265787 | Detection of catabolic genes in indigenous microbial consortia isolated from a diesel-contaminated soil. | Milcic-Terzic J, Lopez-Vidal Y, Vrvic MM, Saval S | Bioresour Technol | 10.1016/s0960-8524(00)00156-5 | 2001 | Base Sequence, Biodegradation, Environmental, DNA Primers/genetics, DNA, Bacterial/genetics/isolation & purification, *Environmental Pollution, *Gasoline, *Genes, Bacterial, Nucleic Acid Hybridization, Polymerase Chain Reaction, Pseudomonas putida/genetics/metabolism, *Soil Microbiology, Soil Pollutants/*metabolism | Metabolism |
Metabolism | 15213740 | A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil. | Luz AP, Pellizari VH, Whyte LG, Greer CW | Can J Microbiol | 10.1139/w04-008 | 2004 | Acinetobacter/genetics, Antarctic Regions, Bacteria/*genetics/*metabolism, Bacterial Proteins/genetics, Brazil, DNA, Bacterial/analysis/isolation & purification, Energy Metabolism, Hydrocarbons/*metabolism, Iron-Sulfur Proteins/genetics, Nucleic Acid Hybridization, Oxygenases/genetics, Polymerase Chain Reaction, Pseudomonas pseudoalcaligenes/genetics, Pseudomonas putida/genetics, Rhodococcus/genetics, *Soil Microbiology | Phylogeny |
Metabolism | 16232508 | Analysis of a simple biodegradation process for the removal of volatile organic chemicals from wastewater based on a gas stripping principle. | Dahlan MH, Xing XH, Yoshikawa Y, Matsumoto K | J Biosci Bioeng | 10.1016/s1389-1723(99)80103-0 | 1999 | ||
16347056 | Bioaccumulation of Germanium by Pseudomonas putida in the Presence of Two Selected Substrates. | Chmielowski J, Klapcinska B | Appl Environ Microbiol | 10.1128/aem.51.5.1099-1103.1986 | 1986 | |||
16347062 | Binding of Germanium to Pseudomonas putida Cells. | Klapcinska B, Chmielowski J | Appl Environ Microbiol | 10.1128/aem.51.5.1144-1147.1986 | 1986 | |||
Enzymology | 16813425 | Iron(III) coordination properties of a pyoverdin siderophore produced by Pseudomonas putida ATCC 33015. | Boukhalfa H, Reilly SD, Michalczyk R, Iyer S, Neu MP | Inorg Chem | 10.1021/ic060196p | 2006 | Ferric Compounds/*chemistry, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Oligopeptides/biosynthesis/*chemistry/isolation & purification, Organometallic Compounds/*chemistry, Potentiometry, Pseudomonas putida/*chemistry/metabolism, Siderophores/biosynthesis/*chemistry/isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectrophotometry, Ultraviolet | Phylogeny |
Genetics | 17370070 | Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies. | Ballerstedt H, Volkers RJ, Mars AE, Hallsworth JE, dos Santos VA, Puchalka J, van Duuren J, Eggink G, Timmis KN, de Bont JA, Wery J | Appl Microbiol Biotechnol | 10.1007/s00253-007-0914-z | 2007 | *Genome, Bacterial, *Genomics, *Oligonucleotide Array Sequence Analysis, Phylogeny, Pseudomonas putida/*genetics, RNA/*genetics | Phylogeny |
18576134 | Development of a membrane-based vapor-phase bioreactor. | Rouhana N, Handagama N, Bienkowski PR | Appl Biochem Biotechnol | 10.1007/BF02920477 | 1997 | |||
18964500 | Radiometric determination of uranium accumulated in bacterial cells. | Chwistek M, Chmielowski J, Tomza I | Talanta | 10.1016/0039-9140(88)80070-5 | 1988 | |||
24190270 | Fate of Pseudomonas putida after release into lake water mesocosms: Different survival mechanisms in response to environmental conditions. | Brettar I, Ramos-Gonzalez MI, Ramos JL, Hofle MG | Microb Ecol | 10.1007/BF00165812 | 1994 | |||
Biotechnology | 32044035 | High-yield and plasmid-free biocatalytic production of 5-methylpyrazine-2-carboxylic acid by combinatorial genetic elements engineering and genome engineering of Escherichia coli. | Gu L, Yuan H, Lv X, Li G, Cong R, Li J, Du G, Liu L | Enzyme Microb Technol | 10.1016/j.enzmictec.2019.109488 | 2019 | Biocatalysis, CRISPR-Cas Systems, Carboxylic Acids/*metabolism, Escherichia coli/*genetics, *Genome, Bacterial, Metabolic Engineering/*methods, Oxygenases/metabolism, Pyrazoles/*metabolism, Substrate Specificity | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1518 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3931) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3931 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
44546 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 2480) | https://www.ccug.se/strain?id=2480 | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
82092 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92520.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
82093 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID112627.1 | StrainInfo: A central database for resolving microbial strain identifiers |