Strain identifier

BacDive ID: 12880

Type strain: No

Species: Pseudomonas putida

Strain Designation: mt-2 PaW1, pWWO

Strain history: ATCC 33015 <-- P. A. Williams mt-2 <-- G. D. Hegeman.

NCBI tax ID(s): 303 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1518

BacDive-ID: 12880

DSM-Number: 3931

keywords: 16S sequence, Bacteria, mesophilic

description: Pseudomonas putida mt-2 PaW1 is a mesophilic bacterium that was isolated from Soil using benzoate as a major carbon source.

NCBI tax id

  • NCBI tax id: 303
  • Matching level: species

strain history

  • @ref: 67770
  • history: ATCC 33015 <-- P. A. Williams mt-2 <-- G. D. Hegeman.

doi: 10.13145/bacdive12880.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas putida
  • full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Bacillus putidus
    20215Arthrobacter siderocapsulatus

@ref: 1518

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudomonadales

family: Micrococcaceae

genus: Pseudomonas

species: Pseudomonas putida

full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895

strain designation: mt-2 PaW1, pWWO

type strain: no

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
44546positivegrowth26mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
44546--+--+-----+--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
1518---+----+-+---++-++++
1518---+----+-+---++-++++
1518---+----+-+---++-++++
44546---+----+-+---++-++++

Isolation, sampling and environmental information

isolation

  • @ref: 44546
  • sample type: Soil using benzoate as a major carbon source

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_239.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_203;99_239&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AB598738
  • Sequence Identity:
  • Total samples: 2133
  • soil counts: 368
  • aquatic counts: 543
  • animal counts: 799
  • plant counts: 423

Safety information

risk assessment

  • @ref: 1518
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas putida (strain JCM 6156 (ATCC 33015)) gene for 16S ribosomal RNA, partial sequenceD379241329ena303
67770Pseudomonas putida gene for 16S rRNA, partial sequence, strain: JCM 6156AB5987381459ena303

External links

@ref: 1518

culture collection no.: DSM 3931, ATCC 33015, NCIB 12182, CCUG 2480, ATCC 23973, JCM 6156, BCRC 10460

straininfo link

@refstraininfo
8209292520
82093112627

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism3794647Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines.McClure NC, Venables WAJ Gen Microbiol10.1099/00221287-132-8-22091986*Adaptation, Physiological, Amines/*metabolism, Biodegradation, Environmental, Plasmids, Pseudomonas/*growth & development, Substrate Specificity, Toluene/metabolism, Toluidines/metabolism
Phylogeny11265787Detection of catabolic genes in indigenous microbial consortia isolated from a diesel-contaminated soil.Milcic-Terzic J, Lopez-Vidal Y, Vrvic MM, Saval SBioresour Technol10.1016/s0960-8524(00)00156-52001Base Sequence, Biodegradation, Environmental, DNA Primers/genetics, DNA, Bacterial/genetics/isolation & purification, *Environmental Pollution, *Gasoline, *Genes, Bacterial, Nucleic Acid Hybridization, Polymerase Chain Reaction, Pseudomonas putida/genetics/metabolism, *Soil Microbiology, Soil Pollutants/*metabolismMetabolism
Metabolism15213740A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil.Luz AP, Pellizari VH, Whyte LG, Greer CWCan J Microbiol10.1139/w04-0082004Acinetobacter/genetics, Antarctic Regions, Bacteria/*genetics/*metabolism, Bacterial Proteins/genetics, Brazil, DNA, Bacterial/analysis/isolation & purification, Energy Metabolism, Hydrocarbons/*metabolism, Iron-Sulfur Proteins/genetics, Nucleic Acid Hybridization, Oxygenases/genetics, Polymerase Chain Reaction, Pseudomonas pseudoalcaligenes/genetics, Pseudomonas putida/genetics, Rhodococcus/genetics, *Soil MicrobiologyPhylogeny
Metabolism16232508Analysis of a simple biodegradation process for the removal of volatile organic chemicals from wastewater based on a gas stripping principle.Dahlan MH, Xing XH, Yoshikawa Y, Matsumoto KJ Biosci Bioeng10.1016/s1389-1723(99)80103-01999
16347056Bioaccumulation of Germanium by Pseudomonas putida in the Presence of Two Selected Substrates.Chmielowski J, Klapcinska BAppl Environ Microbiol10.1128/aem.51.5.1099-1103.19861986
16347062Binding of Germanium to Pseudomonas putida Cells.Klapcinska B, Chmielowski JAppl Environ Microbiol10.1128/aem.51.5.1144-1147.19861986
Enzymology16813425Iron(III) coordination properties of a pyoverdin siderophore produced by Pseudomonas putida ATCC 33015.Boukhalfa H, Reilly SD, Michalczyk R, Iyer S, Neu MPInorg Chem10.1021/ic060196p2006Ferric Compounds/*chemistry, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Oligopeptides/biosynthesis/*chemistry/isolation & purification, Organometallic Compounds/*chemistry, Potentiometry, Pseudomonas putida/*chemistry/metabolism, Siderophores/biosynthesis/*chemistry/isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectrophotometry, UltravioletPhylogeny
Genetics17370070Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies.Ballerstedt H, Volkers RJ, Mars AE, Hallsworth JE, dos Santos VA, Puchalka J, van Duuren J, Eggink G, Timmis KN, de Bont JA, Wery JAppl Microbiol Biotechnol10.1007/s00253-007-0914-z2007*Genome, Bacterial, *Genomics, *Oligonucleotide Array Sequence Analysis, Phylogeny, Pseudomonas putida/*genetics, RNA/*geneticsPhylogeny
18576134Development of a membrane-based vapor-phase bioreactor.Rouhana N, Handagama N, Bienkowski PRAppl Biochem Biotechnol10.1007/BF029204771997
18964500Radiometric determination of uranium accumulated in bacterial cells.Chwistek M, Chmielowski J, Tomza ITalanta10.1016/0039-9140(88)80070-51988
24190270Fate of Pseudomonas putida after release into lake water mesocosms: Different survival mechanisms in response to environmental conditions.Brettar I, Ramos-Gonzalez MI, Ramos JL, Hofle MGMicrob Ecol10.1007/BF001658121994
Biotechnology32044035High-yield and plasmid-free biocatalytic production of 5-methylpyrazine-2-carboxylic acid by combinatorial genetic elements engineering and genome engineering of Escherichia coli.Gu L, Yuan H, Lv X, Li G, Cong R, Li J, Du G, Liu LEnzyme Microb Technol10.1016/j.enzmictec.2019.1094882019Biocatalysis, CRISPR-Cas Systems, Carboxylic Acids/*metabolism, Escherichia coli/*genetics, *Genome, Bacterial, Metabolic Engineering/*methods, Oxygenases/metabolism, Pyrazoles/*metabolism, Substrate SpecificityMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1518Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3931)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3931
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
44546Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2480)https://www.ccug.se/strain?id=2480
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82092Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92520.1StrainInfo: A central database for resolving microbial strain identifiers
82093Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID112627.1StrainInfo: A central database for resolving microbial strain identifiers