Strain identifier
BacDive ID: 12875
Type strain: 
Species: Pseudomonas putida
Strain Designation: 12/5
Strain history: <- F.H. Bernhardt; 12/5
NCBI tax ID(s): 303 (species)
version 8.1 (current version)
General
@ref: 914
BacDive-ID: 12875
DSM-Number: 1868
keywords: Bacteria, mesophilic, human pathogen
description: Pseudomonas putida 12/5 is a mesophilic human pathogen that was isolated from sewage plant.
NCBI tax id
- NCBI tax id: 303
- Matching level: species
strain history
- @ref: 914
- history: <- F.H. Bernhardt; 12/5
doi: 10.13145/bacdive12875.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas putida
- full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus putidus 20215 Arthrobacter siderocapsulatus
@ref: 914
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas putida
full scientific name: Pseudomonas putida (Trevisan 1889) Migula 1895
strain designation: 12/5
type strain: no
Culture and growth conditions
culture medium
- @ref: 914
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 914
- growth: positive
- type: growth
- temperature: 26
- range: mesophilic
Physiology and metabolism
compound production
- @ref: 914
- compound: 4 methoxybenzoate O-demethylase
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | + | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | + | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 914 | - | - | - | + | - | - | - | - | + | - | - | - | - | - | + | + | - | + | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 914
- sample type: sewage plant
- geographic location: Gießen
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Water treatment plant
Safety information
risk assessment
- @ref: 914
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 914
culture collection no.: DSM 1868
straininfo link
- @ref: 82087
- straininfo: 13917
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 11278039 | An aerobic fixed-phase biofilm reactor system for the degradation of the low-molecular weight aromatic compounds occurring in the effluents of anaerobic digestors treating olive mill wastewaters. | Bertin L, Majone M, Di Gioia D, Fava F | J Biotechnol | 10.1016/s0168-1656(01)00236-x | 2001 | Aerobiosis, *Biodegradation, Environmental, *Bioreactors, Chlorophenols/metabolism, Fermentation, Gram-Negative Aerobic Rods and Cocci/metabolism, Hydrocarbons, Aromatic/chemistry/*metabolism, Molecular Weight, Pseudomonas putida/metabolism, Waste Disposal, Fluid/methods, Waste Management/*methods | |
| Metabolism | 11281329 | Biodegradation of hydroxylated and methoxylated benzoic, phenylacetic and phenylpropenoic acids present in olive mill wastewaters by two bacterial strains. | Di Gioia D, Bertin L, Fava F, Marchetti L | Res Microbiol | 10.1016/s0923-2508(00)01171-2 | 2001 | Betaproteobacteria/classification/growth & development/*metabolism, Biodegradation, Environmental, Biotechnology/methods, Culture Media, Hydrocarbons, Aromatic/*metabolism, Industrial Waste, Olive Oil, *Plant Oils, Pseudomonas putida/growth & development/*metabolism, *Waste Disposal, Fluid | Biotechnology |
| Metabolism | 11414330 | Biodegradation of synthetic and naturally occuring mixtures of mono-cyclic aromatic compounds present in olive mill wastewaters by two aerobic bacteria. | Di Gioia D, Fava F, Bertin L, Marchetti L | Appl Microbiol Biotechnol | 10.1007/s002530000554 | 2001 | Bacteria, Aerobic/*metabolism, Biodegradation, Environmental, Chromatography, High Pressure Liquid, Fruit, Gram-Negative Aerobic Rods and Cocci/genetics/metabolism, Hydrocarbons, Aromatic/*metabolism, Hydrocarbons, Cyclic/*metabolism, Industrial Waste, Molecular Sequence Data, Pseudomonas putida/metabolism | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 914 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1868) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1868 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 82087 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13917.1 | StrainInfo: A central database for resolving microbial strain identifiers |