Strain identifier

BacDive ID: 12867

Type strain: Yes

Species: Pseudomonas oleovorans

Strain history: CIP <- 1959, NCIB <- 1950, NCTC <- 1942, ATCC <- A.C. Chandler, Rice Inst., USA

NCBI tax ID(s): 301 (species)

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General

@ref: 487

BacDive-ID: 12867

DSM-Number: 1045

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudomonas oleovorans CCUG 2087 is an obligate aerobe, mesophilic, motile bacterium that was isolated from cutting fluid.

NCBI tax id

  • NCBI tax id: 301
  • Matching level: species

strain history

@refhistory
487<- NCIB <- NCTC <- ATCC <- A.C. Chandler
67770IFO 13583 <-- ATCC 8062 <-- A. C. Chandler.
121411CIP <- 1959, NCIB <- 1950, NCTC <- 1942, ATCC <- A.C. Chandler, Rice Inst., USA

doi: 10.13145/bacdive12867.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas oleovorans
  • full scientific name: Pseudomonas oleovorans Lee and Chandler 1941 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas pseudoalcaligenes

@ref: 487

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas oleovorans

full scientific name: Pseudomonas oleovorans Lee and Chandler 1941 emend. Saha et al. 2010

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.728
6948099.999negative
121411yesnegativerod-shaped

pigmentation

  • @ref: 121411
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
487REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37331MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
121411CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
487positivegrowth26mesophilic
37331positivegrowth30mesophilic
67770positivegrowth30mesophilic
121411positivegrowth30-41
121411nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121411
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12141116947citrate-carbon source
1214114853esculin-hydrolysis
12141117632nitrate+reduction
12141116301nitrite-reduction
12141117632nitrate+respiration

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
121411oxidase+
121411beta-galactosidase-3.2.1.23
121411alcohol dehydrogenase-1.1.1.1
121411gelatinase-
121411amylase-
121411caseinase-3.4.21.50
121411tween esterase-
121411lecithinase-
121411lysine decarboxylase-4.1.1.18
121411ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44482C10:00.210
    44482C12:010.112
    44482C14:00.314
    44482C15:00.515
    44482C16:015.816
    44482C17:00.317
    44482C18:00.418
    44482C10:0 3OH4.211.423
    44482C11:0 ISO 3OH0.112.09
    44482C12:0 3OH3.413.455
    44482C13:0 iso0.212.612
    44482C16:1 ω7c20.415.819
    44482C16:1 ω7c/C15:0 ISO 2OH3.215.85
    44482C17:0 CYCLO1.916.888
    44482C17:0 iso0.416.629
    44482C17:1 ω8c0.516.792
    44482C18:1 ω7c /12t/9t37.417.824
    44482C18:1 ω9c0.117.769
    44482C18:2 ω6,9c/C18:0 ANTE0.417.724
    44482unknown 11.7980.311.798
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
487+-------+------+-+--+
487+-------+------+-+--+
487+-------+------+-+--+
487+-------+------+-+--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121411++----------------+-----------------------------+++--+------+-+++---+-++++-++++++++++--++-+++++-++-

Isolation, sampling and environmental information

isolation

@refsample type
487cutting fluid
67770Cutting fluid
121411Other, Cutting compound, oil-water emulsion

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial
  • Cat3: #Industrial production

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
4871Risk group (German classification)
1214111Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
487P.oleovorans 16S rRNA geneZ766651492ena1218072
487Pseudomonas oleovorans strain ATCC 8062 16S ribosomal RNA gene, partial sequenceAF0947351440ena301
487Pseudomonas oleovorans gene for 16S rRNA, partial sequence, strain: NBRC 13583AB6804501462ena301
67770Pseudomonas oleovorans 16S rRNA gene, complete sequenceD840181526ena301
67770Pseudomonas oleovorans subsp. oleovorans JCM 11598 gene for 16S ribosomal RNA, partial sequenceLC5074441475ena1218129

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas oleovorans NCTC10692GCA_900455615contigncbi301
66792Pseudomonas oleovorans strain NCTC10692301.20wgspatric301
66792Pseudomonas oleovorans strain RS1301.22wgspatric301
66792Pseudomonas oleovorans subsp. oleovorans NBRC 13583 strain NBRC 135831218072.3wgspatric1218072
66792Pseudomonas oleovorans subsp. oleovorans strain DSM 10451218129.3wgspatric1218129
66792Pseudomonas oleovorans oleovorans NBRC 135832811995170draftimg1218072
66792Pseudomonas oleovorans LMG 22292671180034draftimg398198
67770Pseudomonas oleovorans subsp. oleovorans NBRC 13583 = DSM 1045GCA_002091815contigncbi1218072
67770Pseudomonas oleovorans subsp. oleovorans DSM 1045GCA_002197815contigncbi1218129

GC content

  • @ref: 487
  • GC-content: 63.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.754no
anaerobicno98.476no
halophileno76.216no
spore-formingno96.496no
glucose-utilyes90.053yes
flagellatedyes88.121no
aerobicyes89.7no
motileyes87.835no
thermophileno99.423no
glucose-fermentno91.737yes

External links

@ref: 487

culture collection no.: CCUG 2087, IFO 13583, DSM 1045, ATCC 8062, NCIB 6576, NCIMB 6576, NCTC 10692, NBRC 13583, JCM 11598, CECT 4079, CFBP 5589, CIP 59.11, IAM 1508, IMI 349020, KCTC 2872, LMG 2229, NCCB 60081, NRRL B-778, VKM B-1522

straininfo link

  • @ref: 82079
  • straininfo: 588

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism12074088The synthesis of short- and medium-chain-length poly(hydroxyalkanoate) mixtures from glucose- or alkanoic acid-grown Pseudomonas oleovorans.Ashby RD, Solaiman DK, Foglia TAJ Ind Microbiol Biotechnol10.1038/sj.jim.70002312002Fermentation, Gas Chromatography-Mass Spectrometry, Glucose/*metabolism, Hydroxy Acids/*metabolism, Hydroxybutyrates/metabolism, Oxidation-Reduction, Pentanoic Acids/metabolism, Polyesters/metabolism, Polymers/*metabolism, Pseudomonas/*metabolism
Metabolism12382057Poly(ethylene glycol)-mediated molar mass control of short-chain- and medium-chain-length poly(hydroxyalkanoates) from Pseudomonas oleovorans.Ashby RD, Solaiman DK, Foglia TAAppl Microbiol Biotechnol10.1007/s00253-002-1110-92002Alkanes/metabolism, Biopolymers/*metabolism, Chromatography, Gel, Culture Media, Fermentation, Hydroxy Acids/*metabolism, Polyethylene Glycols/*pharmacology, Polymers/metabolism, Pseudomonas/growth & development/*metabolismCultivation
Phylogeny15388721Pseudomonas psychrotolerans sp. nov.Hauser E, Kampfer P, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.03024-02004Animals, Austria, Bacterial Typing Techniques, DNA Fingerprinting, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/biosynthesis, Pseudomonas/*classification/genetics/*isolation & purification/physiology, Pseudomonas oleovorans/classification, Putrescine/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analysis, Ubiquinone/analysisEnzymology
Enzymology15968501Genetic characterization of the poly(hydroxyalkanoate) synthases of various Pseudomonas oleovorans strains.Solaiman DK, Ashby RDCurr Microbiol10.1007/s00284-005-4508-72005Acyltransferases/chemistry/*genetics/metabolism, Amino Acid Sequence, Cloning, Molecular, Molecular Sequence Data, Polyesters/metabolism, Polymerase Chain Reaction, Pseudomonas oleovorans/classification/*enzymology/genetics, Sequence Alignment, Sequence Analysis, DNAMetabolism
Phylogeny19936829Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of Pseudomonas oleovorans ATCC 8062 T.Saha R, Sproer C, Beck B, Bagley SCurr Microbiol10.1007/s00284-009-9540-62009Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/analysis, *Industrial Waste, Molecular Sequence Data, Nucleic Acid Hybridization, Pseudomonas oleovorans/chemistry/*classification/*genetics/isolation & purification, Pseudomonas pseudoalcaligenes/*classification/*genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Sigma Factor/geneticsGenetics
Phylogeny24021726Pseudomonas chengduensis sp. nov., isolated from landfill leachate.Tao Y, Zhou Y, He X, Hu X, Li DInt J Syst Evol Microbiol10.1099/ijs.0.050294-02013Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Solid Waste, Ubiquinone/chemistry, Waste Disposal Facilities, Water Microbiology, Water Pollutants, Chemical/*analysisGenetics
Genetics28798180First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045.Poehlein A, Daniel R, Thurmer A, Bollinger A, Thies S, Katzke N, Jaeger KEGenome Announc10.1128/genomeA.00774-172017
28811756Pseudomonas oleovorans Strain KBPF-004 Culture Supernatants Reduced Seed Transmission of Cucumber green mottle mosaic virus and Pepper mild mottle virus, and Remodeled Aggregation of 126 kDa and Subcellular Localization of Movement Protein of Pepper mild mottle virus.Kim NG, Seo EY, Han SH, Gong JS, Park CN, Park HS, Domier LL, Hammond J, Lim HSPlant Pathol J10.5423/PPJ.OA.03.2017.00472017
Phylogeny29239716Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India.Sudan SK, Pal D, Bisht B, Kumar N, Chaudhry V, Patil P, Sahni G, Mayilraj S, Krishnamurthi SInt J Syst Evol Microbiol10.1099/ijsem.0.0025202017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, India, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, *Water MicrobiologyTranscriptome
Phylogeny29948825Taxonomic description and draft genome of Pseudomonas sediminis sp. nov., isolated from the rhizospheric sediment of Phragmites karka.Behera P, Mahapatra M, Seuylemezian A, Vaishampayan P, Ramana VV, Joseph N, Joshi A, Shouche Y, Suar M, Pattnaik AK, Rastogi GJ Microbiol10.1007/s12275-018-7549-x2018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, Bacterial, *Genome, Bacterial, India, Lakes, Phospholipids/analysis, Phylogeny, Pseudomonas/chemistry/*classification/*genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Rhizosphere, Saline Waters, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny30601113Pseudomonas mangrovi sp. nov., isolated from mangrove soil.Ye Y, Chen C, Ren Y, Wang R, Zhang C, Han S, Ju Z, Zhao Z, Sun C, Wu MInt J Syst Evol Microbiol10.1099/ijsem.0.0031412019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Rhizophoraceae/*microbiology, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32242797Pseudomonas hydrolytica sp. nov., multiple polymer-degrading bacteria isolated from soil in China.Zhou S, Wang Y, Xia H, Liu D, Chen S, Li FInt J Syst Evol Microbiol10.1099/ijsem.0.0041292020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Forests, Genes, Bacterial, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polymers, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistryTranscriptome
Metabolism35689128Control of a pyrimidine ribonucleotide salvage pathway in Pseudomonas oleovorans.Gill R, West TPArch Microbiol10.1007/s00203-022-03016-32022Ammonium Sulfate, Carbon, Cytosine Deaminase, Humans, Nitrogen, *Nucleoside Deaminases/metabolism, *Pseudomonas oleovorans, Pyrimidine Nucleotides, Pyrimidines/metabolism, Ribonucleotides, Succinic Acid/metabolism, Uracil/metabolismPathogenicity

Reference

@idauthorscataloguedoi/urltitle
487Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1045)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1045
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37331Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9749
44482Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2087)https://www.ccug.se/strain?id=2087
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82079Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID588.1StrainInfo: A central database for resolving microbial strain identifiers
121411Curators of the CIPCollection of Institut Pasteur (CIP 59.11)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.11