Strain identifier

BacDive ID: 12807

Type strain: Yes

Species: Pseudomonas alcaligenes

Strain Designation: 1577, 142

Strain history: CIP <- 1984, LMG <- 1973, B. Contopoulos <- N. J. Palleroni <- R. Y. Stanier, Inst. Pasteur, Paris, France: strain 142

NCBI tax ID(s): 1215092 (strain), 43263 (species)

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General

@ref: 12715

BacDive-ID: 12807

DSM-Number: 50342

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas alcaligenes 1577 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from swimming-pool water.

NCBI tax id

NCBI tax idMatching level
43263species
1215092strain

strain history

@refhistory
12715<- ATCC <- R. Hugh, 1577
67770IAM 12411 <-- S. Ikemoto KS 0021 <-- AJ 2085 <-- ATCC 14909 <-- R. Hugh 1577.
116206CIP <- 1984, LMG <- 1973, B. Contopoulos <- N. J. Palleroni <- R. Y. Stanier, Inst. Pasteur, Paris, France: strain 142

doi: 10.13145/bacdive12807.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas alcaligenes
  • full scientific name: Pseudomonas alcaligenes Monias 1928 (Approved Lists 1980)

@ref: 12715

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas alcaligenes

full scientific name: Pseudomonas alcaligenes Monias 1928

strain designation: 1577, 142

type strain: yes

Morphology

cell morphology

  • @ref: 116206
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 116206
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12715REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40285MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116206CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116206CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
12715positivegrowth26mesophilic
40285positivegrowth30mesophilic
44372positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
116206positivegrowth10-41
116206nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44372aerobe
116206obligate aerobe

compound production

  • @ref: 12715
  • compound: poly(ß-hydroxyoctanoic acid)

halophily

@refsaltgrowthtested relationconcentration
116206NaClpositivegrowth0-2 %
116206NaClnogrowth4 %
116206NaClnogrowth6 %
116206NaClnogrowth8 %
116206NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
11620616947citrate+carbon source
1162064853esculin-hydrolysis
11620617632nitrate+reduction
11620616301nitrite-reduction

antibiotic resistance

  • @ref: 116206
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11620635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
116206oxidase+
116206beta-galactosidase-3.2.1.23
116206alcohol dehydrogenase+1.1.1.1
116206gelatinase-
116206amylase-
116206DNase-
116206caseinase-3.4.21.50
116206catalase+1.11.1.6
116206tween esterase+
116206lecithinase+
116206lipase+
116206lysine decarboxylase-4.1.1.18
116206ornithine decarboxylase-4.1.1.17
116206protease+
116206tryptophan deaminase-
116206urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44372C10:03.410
    44372C11:00.611
    44372C12:06.712
    44372C13:00.413
    44372C14:04.614
    44372C15:01.815
    44372C16:012.616
    44372C10:0 3OH511.423
    44372C12:0 3OH313.455
    44372C16:1 ω7c28.715.819
    44372C17:1 ω6c0.716.862
    44372C17:1 ω8c1.316.792
    44372C18:1 ω7c /12t/9t30.617.824
    44372C18:2 ω6,9c/C18:0 ANTE0.717.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116206-+++++--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
44372+--------------+-++-+
12715+--------------+-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116206------------------------------------------------+++--+------------------++-+++++++-+----++++++--++-

Isolation, sampling and environmental information

isolation

@refsample typesampling dateisolation date
12715swimming-pool water
44372Water,swimming pool1961
67770Swimming-pool water
116206Environment, Swimming-pool water1961

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Water reservoir (Aquarium/pool)
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_76.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_69;99_76&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: Z76653
  • Sequence Identity:
  • Total samples: 1600
  • soil counts: 779
  • aquatic counts: 384
  • animal counts: 276
  • plant counts: 161

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
127152Risk group (German classification)
1162061Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas alcaligenes strain ATCC 14909 16S ribosomal RNA gene, partial sequenceAF0947211459ena43263
20218Pseudomonas alcaligenes strain BCRC 13909 16S-23S ribosomal RNA intergenic spacer, complete sequenceEU014521499ena43263
20218Pseudomonas alcaligenes culture-collection CFBP:2437 16S ribosomal RNA gene, partial sequenceHM1902311442ena43263
20218Pseudomonas alcaligenes 16S rRNA gene, complete sequenceD840061530ena43263
20218P.alcaligenes 16S rRNA geneZ766531492ena43263
20218Pseudomonas alcaligenes gene for 16S rRNA, partial sequence, strain: NBRC 14159AB6805671462ena43263

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas alcaligenes NBRC 141591215092.3wgspatric1215092
66792Pseudomonas alcaligenes strain NCTC1036743263.13wgspatric43263
66792Pseudomonas alcaligenes NBRC 141592600255064draftimg1215092
67770Pseudomonas alcaligenes NBRC 14159GCA_000467105contigncbi1215092
67770Pseudomonas alcaligenes NCTC10367GCA_900455475contigncbi43263

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.384no
gram-positiveno98.488no
anaerobicno97.663yes
aerobicyes90.892yes
halophileno90.556no
spore-formingno94.108no
thermophileno99.186yes
glucose-utilyes60.669yes
flagellatedyes87.65no
glucose-fermentno90.234no

External links

@ref: 12715

culture collection no.: DSM 50342, ATCC 14909, NCTC 10367, CCUG 1425 A, CCM 2655, LMG 1224, JCM 20561, BCRC 13909, CCUG 1425, CECT 929, CIP 101034, IAM 12411, ICPB 2749-12, IFO 14159, JCM 5967, LMG 2120, NBRC 14159, NCIMB 9945, NCPPB 1970, VKM B-2171, IMET 11155

straininfo link

  • @ref: 82017
  • straininfo: 13751

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1883229Pyrimidine base and ribonucleoside utilization by the Pseudomonas alcaligenes group.West TPAntonie Van Leeuwenhoek10.1007/BF005836791991Amidohydrolases/metabolism, Culture Media, Cytosine Deaminase, Dihydrouracil Dehydrogenase (NADP), N-Glycosyl Hydrolases/metabolism, Nucleoside Deaminases/metabolism, Oxidoreductases/metabolism, Pseudomonas/*metabolism, Pyrimidines/*metabolism, Ribonucleosides/*metabolismCultivation
Metabolism12596245Comparison of aspartate transcarbamoylase regulation in Pseudomonas alcaligenes and Pseudomonas mendocina.Santiago MF, West TPJ Basic Microbiol10.1002/jobm.2003900082003Aspartate Carbamoyltransferase/*metabolism, Diphosphates/metabolism, Nucleotides/metabolism, Phosphates/metabolism, Pseudomonas/classification/*enzymologyEnzymology
Metabolism12906394Effect of carbon source on pyrimidine biosynthesis in Pseudomonas alcaligenes ATCC 14909.Santiago MF, West TPMicrobiol Res10.1078/0944-5013-001722003Aspartate Carbamoyltransferase/metabolism, Culture Media, Dihydroorotase, Dihydroorotate Dehydrogenase, Glucose/metabolism, Orotate Phosphoribosyltransferase/metabolism, Orotidine-5'-Phosphate Decarboxylase/metabolism, Oxidoreductases/metabolism, *Oxidoreductases Acting on CH-CH Group Donors, Pseudomonas/*enzymology/*metabolism, Pyrimidines/*biosynthesis, Succinic Acid/metabolismEnzymology
Phylogeny23918786Pseudomonas guguanensis sp. nov., a gammaproteobacterium isolated from a hot spring.Liu YC, Young LS, Lin SY, Hameed A, Hsu YH, Lai WA, Shen FT, Young CCInt J Syst Evol Microbiol10.1099/ijs.0.047712-02013Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Taiwan, Ubiquinone/chemistryGenetics
Metabolism25681184Arsenic methylation and volatilization by arsenite S-adenosylmethionine methyltransferase in Pseudomonas alcaligenes NBRC14159.Zhang J, Cao T, Tang Z, Shen Q, Rosen BP, Zhao FJAppl Environ Microbiol10.1128/AEM.03804-142015Amino Acid Sequence, Arsenites/*metabolism, Bacterial Proteins/chemistry/*genetics/metabolism, Biotransformation, Methyltransferases/chemistry/*genetics/metabolism, Molecular Sequence Data, Pseudomonas alcaligenes/*enzymology/*genetics/metabolism, Sequence AlignmentGenetics
Genetics26051509Whole genome sequences of a free-living Pseudomonas sp. strain ML96 isolated from a freshwater Maar Lake.Li X, Blom J, Zeng YMar Genomics10.1016/j.margen.2015.05.0162015China, Genome, Bacterial, Lakes/*microbiology, Molecular Sequence Data, Phylogeny, Pseudomonas/*classification/*genetics, *Water MicrobiologyPhylogeny
Phylogeny29239716Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India.Sudan SK, Pal D, Bisht B, Kumar N, Chaudhry V, Patil P, Sahni G, Mayilraj S, Krishnamurthi SInt J Syst Evol Microbiol10.1099/ijsem.0.0025202017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, India, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, *Water MicrobiologyTranscriptome
33072469Bacillus subtilis TR47II as a source of bioactive lipopeptides against Gram-negative pathogens causing nosocomial infections.de Souza Freitas F, Coelho de Assis Lage T, Ayupe BAL, de Paula Siqueira T, de Barros M, Totola MR3 Biotech10.1007/s13205-020-02459-z2020
Phylogeny34762579Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan.Yamada K, Sasaki M, Aoki K, Nagasawa T, Murakami H, Ishii M, Shibuya K, Morita T, Ishii Y, Tateda KInt J Syst Evol Microbiol10.1099/ijsem.0.0051152021Bacterial Typing Techniques, Base Composition, *Burns/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Humans, Japan, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Skin/*microbiologyPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12715Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50342)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50342
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40285Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12564
44372Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 1425 A)https://www.ccug.se/strain?id=1425
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82017Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13751.1StrainInfo: A central database for resolving microbial strain identifiers
116206Curators of the CIPCollection of Institut Pasteur (CIP 101034)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101034