Strain identifier

BacDive ID: 12804

Type strain: No

Species: Pseudomonas aeruginosa

Strain history: CIP <- 1952, Bass

NCBI tax ID(s): 287 (species)

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General

@ref: 17914

BacDive-ID: 12804

DSM-Number: 25123

keywords: Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Pseudomonas aeruginosa DSM 25123 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from wound.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

@refhistory
17914<- CIP <- isolated 1952 by Bass
418891952, Bass
116082CIP <- 1952, Bass

doi: 10.13145/bacdive12804.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 17914

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

type strain: no

Morphology

cell morphology

  • @ref: 116082
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 116082
  • production: yes
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17914TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
41889MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
17914TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
17914COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
116082CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116082CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17914positivegrowth28mesophilic
41889positivegrowth25mesophilic
17914positivegrowth30mesophilic
116082positivegrowth10-41
116082nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116082
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836817632nitrate-reduction
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11608217108D-arabinose-degradation
11608215824D-fructose-degradation
11608217634D-glucose-degradation
11608216024D-mannose-degradation
11608265327D-xylose-degradation
11608217057cellobiose-degradation
11608217716lactose-degradation
11608217306maltose-degradation
11608217814salicin-degradation
11608217992sucrose-degradation
11608216947citrate+carbon source
1160824853esculin-hydrolysis
11608217632nitrate+reduction
11608216301nitrite+reduction
11608217632nitrate+respiration

antibiotic resistance

  • @ref: 116082
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11608235581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
6836815688acetoin-
6836835581indole-
11608215688acetoin-
11608217234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase+3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116082oxidase+
116082beta-galactosidase-3.2.1.23
116082alcohol dehydrogenase+1.1.1.1
116082gelatinase+/-
116082amylase-
116082DNase-
116082caseinase+3.4.21.50
116082catalase+1.11.1.6
116082tween esterase+
116082lecithinase+
116082lipase+
116082lysine decarboxylase-4.1.1.18
116082ornithine decarboxylase-4.1.1.17
116082urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116082--------------------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUNO2N2
116082--------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
17914---++-+-+--++-+++++-+
17914+--++-+-+--++-+++++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116082++-----------------+------+----+-+--------------+++--+--+--++-++++-+---+++++++-++++++--++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
17914wound
116082Wound1918

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Other
  • Cat3: #Wound

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
17914yesyes2Risk group (German classification)
1160822Risk group (French classification)

External links

@ref: 17914

culture collection no.: DSM 25123, CECT 4080, CIP A22, LMG 10639, NCIMB 13056, WDCM 00027, NCTC 13646

straininfo link

  • @ref: 82014
  • straininfo: 101724

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity14529639Efficacy of some neutralizers in suspension tests determining the activity of disinfectants.Espigares E, Bueno A, Fernandez-Crehuet M, Espigares MJ Hosp Infect10.1016/s0195-6701(03)00238-x2003Colony Count, Microbial, Disinfectants/antagonists & inhibitors/*pharmacology, Drug Resistance, Microbial, Gram-Negative Bacteria/*drug effects, Gram-Positive Bacteria/*drug effects, Outcome Assessment, Health Care
Pathogenicity32004609The anti-tumor activity of exopolysaccharides from Pseudomonas strains against HT-29 colorectal cancer cell line.Tahmourespour A, Ahmadi A, Fesharaki MInt J Biol Macromol10.1016/j.ijbiomac.2020.01.2682020Antineoplastic Agents/*pharmacology, Colorectal Neoplasms/*pathology, Flow Cytometry, HT29 Cells, Humans, Polysaccharides, Bacterial/isolation & purification/*pharmacology, Pseudomonas aeruginosa/*chemistry, Spectroscopy, Fourier Transform InfraredEnzymology
35684475Antioxidant and Antimicrobial Potencies of Chemically-Profiled Essential Oil from Asteriscus graveolens against Clinically-Important Pathogenic Microbial Strains.Aljeldah MMMolecules10.3390/molecules271135392022Anti-Bacterial Agents/chemistry/pharmacology, *Anti-Infective Agents/chemistry/pharmacology, Antioxidants/chemistry/pharmacology, *Asteraceae, Candida albicans, Escherichia coli, Gas Chromatography-Mass Spectrometry, Microbial Sensitivity Tests, *Oils, Volatile/chemistry/pharmacology, Pseudomonas aeruginosa, Staphylococcus aureus
36172499Antimicrobial and cytotoxic effects of marine sponge extracts Agelas clathrodes, Desmapsamma anchorata and Verongula rigida from a Caribbean Island.Piron J, Betzi S, Pastour J, Restouin A, Castellano R, Collette Y, Tysklind N, Smith-Ravin J, Priam FPeerJ10.7717/peerj.139552022

Reference

@idauthorscataloguedoi/urltitle
17914Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25123)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25123
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41889Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12645
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
82014Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID101724.1StrainInfo: A central database for resolving microbial strain identifiers
116082Curators of the CIPCollection of Institut Pasteur (CIP A22)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20A22