Strain identifier

BacDive ID: 12802

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PA399

Strain history: <- C. Schoen, Univ. Würzburg, Institut f. Hygiene und Mikrobiologie; PA399 <- J. Elias

NCBI tax ID(s): 287 (species)

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General

@ref: 17705

BacDive-ID: 12802

DSM-Number: 24599

keywords: Bacteria, mesophilic, antibiotic resistance, human pathogen

description: Pseudomonas aeruginosa PA399 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from urine.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 17705
  • history: <- C. Schoen, Univ. Würzburg, Institut f. Hygiene und Mikrobiologie; PA399 <- J. Elias

doi: 10.13145/bacdive12802.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 17705

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: PA399

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17705NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
17705TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
17705COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

  • @ref: 17705
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
17705Mueller-Hinton Agar371aerob0000000024121412022-240100001400016101880028-300000014-16
17705Mueller Hinton37aerob00008000261414100220000014-16000161018-201000320000016-18

observation

  • @ref: 17705
  • observation: ESBL

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369arginine dihydrolase+3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
17705---++/--+-+--++-+++++-+
17705+--++-+-+--++-+++++-+
17705+--++++-+--++-+++++-+

Isolation, sampling and environmental information

isolation

  • @ref: 17705
  • sample type: urine
  • geographic location: Würzburg
  • country: Germany
  • origin.country: DEU
  • continent: Europe
  • latitude: 49.7924
  • longitude: 9.933

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Urine

Safety information

risk assessment

  • @ref: 17705
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 17705

culture collection no.: DSM 24599

straininfo link

  • @ref: 82012
  • straininfo: 407610

Reference

@idauthorscataloguedoi/urltitle
17705Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24599)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24599
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
82012Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID407610.1StrainInfo: A central database for resolving microbial strain identifiers